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Offlinejakesta1
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transferring?
    #15180312 - 10/05/11 08:09 AM (13 years, 3 months ago)

He has plates of agar with contams and wants to transfer the clean myc to a new agar dish. Anyone know how this is done or give me a link?thanx much


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180322 - 10/05/11 08:12 AM (13 years, 3 months ago)

its common for people to take unclean cultures and use multiple agar transfers to clean it up. so you have a good chance

http://www.mushroomvideos.com/Agar-Petri-Dishes

watch this it will show you how to choose a sector to transfer

good luck.


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15180415 - 10/05/11 08:54 AM (13 years, 3 months ago)

I cant really watch videos because I have dialup not by choice there is not a option for high speed in the town I live in. But do I just cut a small Peace of clean myc agar and all from the plate and transfer it to a unused plate?


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180427 - 10/05/11 09:00 AM (13 years, 3 months ago)

look at the growth patters of the myc. take the strongest looking strand. try and be as precise as you and only get one strand. and if your luck you should have a perfectly clean isolate.


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15180504 - 10/05/11 09:27 AM (13 years, 3 months ago)

Do you mean one little tiny strand of myc? Sorry about the dumb questions but im a noob.


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180512 - 10/05/11 09:31 AM (13 years, 3 months ago)

yes try and be precise


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15180541 - 10/05/11 09:40 AM (13 years, 3 months ago)

When do I do this? At the first sign of contam? Thanx your really helping me out all considering the dial up makes it takes three months to go and look at multiple web pages.


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180578 - 10/05/11 09:52 AM (13 years, 3 months ago)

once you start to see Ryzimorphs *the stringy segments that look like little threads* cut one out and put it in the center of a fresh agar plate .

can you give a quick description of the contam your speaking of


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15180910 - 10/05/11 11:22 AM (13 years, 3 months ago)

one is like orangeish not fuzzy and the other is redish pink


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Offlinejakesta1
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Re: transferring? [Re: jakesta1]
    #15180929 - 10/05/11 11:27 AM (13 years, 3 months ago)

We got the mushies from horse manure.


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180961 - 10/05/11 11:36 AM (13 years, 3 months ago)

sounds like you have lipstick mold growing not myc

https://files.shroomery.org/files/06-24/069284222-contam.jpg

thats a picture of lipstick mold in a pf jar

and yellow doesnt sound right either.

i dont think you will have much luck with those cultures. infact you should probibly throw them outside somewhere cause they could cause heath issues.


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15180985 - 10/05/11 11:41 AM (13 years, 3 months ago)

so chuck the red mold one and and orange mold one. But the others dont have it and there is Rhizomorphs growing from under the pieces of mushie in to the agar thats what I transfer right?


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180987 - 10/05/11 11:41 AM (13 years, 3 months ago)



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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15180997 - 10/05/11 11:44 AM (13 years, 3 months ago)

correct. is it growing out far? like the diameter of a ping pong ball?

smaller/bigger?

it might be easier for you to isolate a singal hyphae is let it grow out


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15181032 - 10/05/11 11:52 AM (13 years, 3 months ago)

smaller. I would like to show you a pic but I dont wana open them up and contam even more plus my cam is shirty and I dont have a flowhood.


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15181060 - 10/05/11 11:59 AM (13 years, 3 months ago)

you need to make a glove box then
or a still air box

that way you can at least minimize your contamination chances.


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15181189 - 10/05/11 12:28 PM (13 years, 3 months ago)

We have one. You know we got the mushie from the wild so there is bound to be a contam. But one looks really nice.

this is the bad one.


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Offlinejimmyjame1
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Re: transferring? [Re: jakesta1]
    #15181409 - 10/05/11 01:16 PM (13 years, 3 months ago)

yah the one on the right looks pretty good. use a small strand from that one.


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Offlinejakesta1
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Re: transferring? [Re: jimmyjame1]
    #15182944 - 10/05/11 06:56 PM (13 years, 3 months ago)

That whole plate is gone with the wind.If you look close thy both look to have a dark yellow contam. IDK. We went with the better looking plates. And we stuck the inoculation loop in the agar and twisted it and pulled out a nice little chunk from the cleanest healthiest section


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:mushroom2: This is a story im telling. Nothing is true that I right. You might say im a lier.

Edited by jakesta1 (10/05/11 07:01 PM)

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OfflineRogerRabbitM
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Re: transferring? [Re: jakesta1]
    #15183441 - 10/05/11 08:24 PM (13 years, 3 months ago)

Quote:

jakesta1 said:
I cant really watch videos because I have dialup not by choice there is not a option for high speed in the town I live in. But do I just cut a small Peace of clean myc agar and all from the plate and transfer it to a unused plate?




Same as here, but it's almost 40 miles of old copper to the nearest switch, so dial-up won't even work.  I have to use satellite.  It's expensive and slow.  Only about five or so times dial up speed at best.  You might look into it as an option though.  You still have to start a video and click pause.  It's not even fast enough to watch youtube videos without constantly buffering.
RR


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Offlinejimmyjame1
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Re: transferring? [Re: RogerRabbit]
    #15183860 - 10/05/11 09:44 PM (13 years, 3 months ago)

thats a shame RR. does his culture look like its gonna be useable? am i on the right track with the advice im giving?


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