Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Myyco.com APE Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinespyro
Stranger
 User Gallery
Registered: 10/03/11
Posts: 7
Last seen: 12 years, 6 months
Possible contams? Multple Monos! Nice surprise!
    #15175005 - 10/04/11 01:59 AM (13 years, 3 months ago)

Well, lately I've been experiementing with various teks in more sort of exploratory process in finding out how this wonderful fungus works!

So far I've been able to successfully fruit off of PF cakes, and I had a run a little while ago with my old PC (very small, fit ONE quart jar...sideways...) that I tried with WBS...but the jars stalled and never colonized (moved around too much, etc).
Now I have the proper tools, my own place (very essential!), and a bit more experience :smile:

Now, I'm running a few different strains to see if there are any differences in them that I can tell (everyone has their own perspective and processing of reality), and what I can learn from the different ones, and so I can play with a bunch of different teks.  Now, I turned half of my spore syringes into LCs, which went well, tested them with PFs to see if there was any base contams from the LCs and not a single one came up with anything!  W00t!
Besides the LCs, all of them have been colonizing WBS (soaked for 18 hours, strained thoroughly, PC'ed for 90 min) in either pint or quart jars (the differences are for my various experiments...don't really have a control group now that I think of it, just a lot of experiments :laugh: ).  From there I've either G2G'd a few of them, but all of them are making their way or have already into various sized mono tubs with a coir+verm sub.  After making the mono tubs, mixing the WBS with the coir+verm, I topped with another thin layer of the coir+verm mix to hide any of the seed from the surface.  Then I've wrapped all the tubs in large garbage bags to get the coir+verm sub colonized.  I also have a HEPA filter running in the room at all times, and try to keep rather sterile practices (though the bird always doesn't...:frown: )

So to recap I have: different strains, different methods used in the process, and different tubs bring fruited pertaining to the different methods used.  I have a lot of experiments in a sorta shotgun assembly with no real control groups...but this is just my method of learning really...collect a lot of information with as many variables as possible, then process and analyze the data for a better understand to the inner workings!

Back to my intermediate experience and lack of controls...I need help!

Went to check up on my tubs, and found a few variations amongst them.  Some I think are cobweb'd, some are rather nicely progressing, some are rather unknown to me, and one's fruiting!

This one's fruiting, though I have no clue as to why...it has spent the same time as the other tubs of similar size, it has also been enclosed in the black garbage bags (at the bottom of the stack too!) rather well sealed.  My understanding is that for fruiting FAE and some light are needed but it really had neither!  These don't look normal either though...kinda stunted and wonky-ways...they're KSSS cubes.
FRUITED KSSS #9?!?!? (password: lolpenis)

Anyone can lend a clue as to why they would have fruited and as such?  Currently, they are bag-less receiving light and FAE...should I stop and not keep trying to fruit this for some reason?

And in writing this novel, I took a look at my APE wbs quarts in my incubator...story with the APE...last time I had APE it didn't take at all, so I used the whole syringe in 1pt WBS :laugh:  Remember, I'm just doing this to learn and experiment really...I know most people would think that crazy or unreasonable but it seemed to increase my odds...and it did.  Colonized quickest outta any...then G2G'd to 4 other pint jars, then to 4 quart jars...after the first shake most of them have seemed to stall and one jar actually has some not so friendly yellow stuff in it, and some wet rot at the bottom too :frown:
Oh well...I can get more APEs in this jungle! :laugh:

Moving on...
I'll try to keep this short from here on...(thanks to you for still reading whoever so may be!)

This tub seems to be colonizing rather well so far, but it has some water forming on the surface and in a few of the water droplets I noticed some yellow coloring...contam or just metabolites?

Yellowing? (password: lolpenis)

And this one actually isn't a contam issue...I messed this one up while moving it too quickly...the coir shifted into a hill formation...can I shift it back or would that hurt it?  Am I better off letting iit colonize as it is and fruiting it as it is too?

Down the rabbit hole or up bunny hill? (again, password: lolpenis)

Thanks to anyone who responds or anyone who even reads this far!
I have a few more tubs with a few more contam questions I'd like to ask but maybe I'll ask those if I get a response to this long ass post, haha.

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Possible contams? Multple Monos! Nice surprise! [Re: spyro]
    #15175080 - 10/04/11 02:50 AM (13 years, 3 months ago)

Quote:

This one's fruiting, though I have no clue as to why...it has spent the same time as the other tubs of similar size, it has also been enclosed in the black garbage bags (at the bottom of the stack too!) rather well sealed.  My understanding is that for fruiting FAE and some light are needed but it really had neither!  These don't look normal either though...kinda stunted and wonky-ways...they're KSSS cubes.



If the mycelium is healthy and can't colonize any more substrate, it will sometimes start fruiting even without being induced to do so. It could be too much moisture, or some kind of bacteria, maybe the ph is to basic or acidic, there are a lot of possibilities but I wouldn't worry too much. It may finish colonizing later even though it has begun fruiting or it may not. Either way you'll probably get a good flush or 2.

Quote:

This tub seems to be colonizing rather well so far, but it has some water forming on the surface and in a few of the water droplets I noticed some yellow coloring...contam or just metabolites?



It's kind of hard to make out but metabolites would be my guess.

Quote:

And this one actually isn't a contam issue...I messed this one up while moving it too quickly...the coir shifted into a hill formation...can I shift it back or would that hurt it?  Am I better off letting iit colonize as it is and fruiting it as it is too?



I would leave it be unless there are spots where the spawn is exposed, you would want to patch those up.


--------------------

Extras: Filter Print Post Top
Offlinespyro
Stranger
 User Gallery
Registered: 10/03/11
Posts: 7
Last seen: 12 years, 6 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: Kizzle]
    #15175747 - 10/04/11 08:58 AM (13 years, 3 months ago)

Thanks for the backup Kizzle!
Hmm, and makes sense, kind of like an auto-flower sorta deal...but the weirder part is that all of the tub isn't even really fully colonized...oh well!
And any real reason to patch up the spawn otherwise?  Something wrong with having colonized WBS on the surface?  I obviously have run all my monotubs with this being done anyway (the added layer of coir+verm at the top), but guess I just wanna better understanding!

Also, now that I'm looking at more monos, I'm noticing that most people don't have the bags taped up all the way up the sides...just a few to hold the bags up...would this be a better idea?  Would water pool in the back/under the bags and be an issue with contams, or would it just keep the RH a bit better?  Just I not worry and keep taping my bags all the way up?  It's funny cause I use Gorrilla tape too, and I still see some myc colonizing behind the tape...there are even a few pins between the tape and the tub! :laugh:

Anywho, back to contams...
There are three small tubs displaying some small deformed pins from what it seems...this one seems to be colonizing, but at the same time I'm seeing what is rather strange pins and fuzz...or maybe a contam that I'm growing =S  Increase FAE (take some polyfil out?), spray with diluted H2O2 treatment?
Tub 1 of odd pins and fuzz (password: lolpenis)
Tub 2 with strange pins + more fuzz
I can also tell there isn't as much condensation in the tubs with the odd pins/fuzz.  Should the H2O2 treatment help RH as well?
Tub 3 of skinny pins/fuzz (password: lolpenis)

MUTANTS?!?!
This tub seems to be colonizing pretty alright...I don't see any signs of real contams or anything, but the growth isn't entirely normal either...this I already have some mutated fruit bodies.  You can kinda see them in the middle (long round loopy thing), and there are two smaller more linear looking ones to the right of it.  Sorry bout the shit picture =S  I'm thinking these are rather premature and mutated fruit bodies (seemingly enough it seems some mutations come from the petrol based shits, even though pretty much everything is a petrol derivative nowadays...and I do spray the shit outta my place with Lysol :laugh: )...should I remove them to prevent them from rotting when the rest fruits?

Some mutants in the middle (password: lolpenis)
Same tub, some more mutants (password: lolpenis)

Now I have a few other questions but they're better suited for other parts of this forum...time to explore!

Thanks again anyone!

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Possible contams? Multple Monos! Nice surprise! [Re: spyro]
    #15176727 - 10/04/11 01:21 PM (13 years, 3 months ago)

It wouldn't necessarily cause problems, but there are going to be mold spores landing on the surface and the spawn, even after it's colonized, is more vulnerable to contamination than the bulk sub.

I really can't for sure, maybe someone else can, about the these other tubs. Mycelium can look a bit different depending on the growing conditions, ie FAE, humidity. If you think it might be a contam, I'd take a close look at the color and how fast it is growing. As for pins growing weird, I'm guessing you don't have an overhead light?

Extras: Filter Print Post Top
Offlinetanman1990
Intermediate Mycologist
Male User Gallery


Registered: 02/28/11
Posts: 217
Last seen: 9 years, 4 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: Kizzle]
    #15177898 - 10/04/11 07:21 PM (13 years, 3 months ago)

The reason you lack replies is that you keep linking to pictures... Just attach the damn things and you will get far more help. When I can see what is in question my answers are far more likely to help. So, post up the pics and I'll weigh in.


--------------------
My Current Bulk Monotub(3rd Flush UPDATE 10/10/11):
http://www.shroomery.org/forums/showflat.php/Number/14973087

Disclaimer: I'm a liar and just want to prove how cool I am by pretending to grow mushroom. I've never done anything involving mycology in my life.

Extras: Filter Print Post Top
Offlinespyro
Stranger
 User Gallery
Registered: 10/03/11
Posts: 7
Last seen: 12 years, 6 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: tanman1990]
    #15178357 - 10/04/11 08:37 PM (13 years, 3 months ago)

Well in that case...let's see how this attaching thing works...used to more commercial bb apps...vb...phpbb...etc...

Tub 1 of strange pins...

Tub 2...

Tub 3...


Mutant loops...


Extras: Filter Print Post Top
Offlinetanman1990
Intermediate Mycologist
Male User Gallery


Registered: 02/28/11
Posts: 217
Last seen: 9 years, 4 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: spyro]
    #15178447 - 10/04/11 08:57 PM (13 years, 3 months ago)

Welllll, first 3 pics look normal to me(for a tub fruiting before 100% colonization) from what I see. The last 2... Something I have no encountered before, I would search the forums or wait and see if a more experienced cultivator can help you figure out what those "loops" are. Welcome to the forum, check out all of the new areas for you to explore!


--------------------
My Current Bulk Monotub(3rd Flush UPDATE 10/10/11):
http://www.shroomery.org/forums/showflat.php/Number/14973087

Disclaimer: I'm a liar and just want to prove how cool I am by pretending to grow mushroom. I've never done anything involving mycology in my life.

Extras: Filter Print Post Top
Offlinetanman1990
Intermediate Mycologist
Male User Gallery


Registered: 02/28/11
Posts: 217
Last seen: 9 years, 4 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: tanman1990]
    #15178458 - 10/04/11 08:59 PM (13 years, 3 months ago)

Also, looks like it needs more FAE(due to the fluffy growth on top. I would recommend making sure your polyfill isnt too tight, and that there are holes about an inch above substrate level to insure proper air exchange. I sometimes even set up a rotating fan a foot or so away from the corner of my tub and let it do its thing.


--------------------
My Current Bulk Monotub(3rd Flush UPDATE 10/10/11):
http://www.shroomery.org/forums/showflat.php/Number/14973087

Disclaimer: I'm a liar and just want to prove how cool I am by pretending to grow mushroom. I've never done anything involving mycology in my life.

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Possible contams? Multple Monos! Nice surprise! [Re: tanman1990]
    #15178561 - 10/04/11 09:16 PM (13 years, 3 months ago)

I've gotten the loops before. It was in a similar situation. They started fruiting before all the coir/verm was colonized. In an attempt to get them to finish colonizing I tried keeping them in the dark, which didn't really work, but the mushrooms that had grown with 24 hours dark grew into loops like that. When I finally put it back out into the light they started growing normal again.

Extras: Filter Print Post Top
Offlinespyro
Stranger
 User Gallery
Registered: 10/03/11
Posts: 7
Last seen: 12 years, 6 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: Kizzle]
    #15181614 - 10/05/11 02:16 PM (13 years, 3 months ago)

@tanman
Thanks for the input!  I've got a HEPA filter on full blast about 6 inches away from the tubs and I've got an oscillating fan in the room on the tubs.  Took two of the tubs from the first 3 set and took the bags off, gonna see what fruiting them will do!  Hopefully fatten up those wonky pins!

And as far as the fluffy/FAE bit, I've only got the holes at the top of the tubs.  Now that I'm actually applying thought to it, I can see the benefit of having two layered levels of holes...if you're trying to emulate a small biosphere then you have to account for gas exchange/FAE and the easiest way to move cold air in and hot air out (from the metabolic process) is with the two layers shifting pressure...oh well, I'll remember that when constructing the next set :smile:

@Kizzle
With the loopy tub, after looking at it again this morning it seems that they were in fact fruits (they have tiny tiny caps now).  So I picked them out to prevent rot and now it's soaking up some light :smile:
Though, I've also noticed that along most of the edge there's some yellowing liquid...just noticed it today so I'll be keeping an eye on it to see if it's met

And the tub I opened with the fruit bodies already growing (on OP), that one's really taking off...in a few hours these things have SHOT UP!  Or sideways really...is there any issue with them being sideways?  A lot of them a course-correcting now that the light is actually introduced above them, but there are still some massive ones that are liking to stretch and roll on the others :smile:

And as a side note...would it REAAAALLY be that big of an issue to even out that hilly sub?  Is the risk only that more surface area and air would be tossed so a higher risk of contams?  Or would it actually damage the myc too much?

Thanks again guys!

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Possible contams? Multple Monos! Nice surprise! [Re: spyro]
    #15182454 - 10/05/11 05:34 PM (13 years, 3 months ago)

I don't know if it'd be a big issue to fix it. I was just thinking you should avoid damaging the mycelium anymore than need be. If it's bothering you just do it, will probably turn out fine.

Extras: Filter Print Post Top
Offlinetanman1990
Intermediate Mycologist
Male User Gallery


Registered: 02/28/11
Posts: 217
Last seen: 9 years, 4 months
Re: Possible contams? Multple Monos! Nice surprise! [Re: Kizzle]
    #15192059 - 10/07/11 02:52 PM (13 years, 3 months ago)

I think you should add holes at just above casing level. The standard see on most monos is to have 4 holes at casing level and 2 upper holes


--------------------
My Current Bulk Monotub(3rd Flush UPDATE 10/10/11):
http://www.shroomery.org/forums/showflat.php/Number/14973087

Disclaimer: I'm a liar and just want to prove how cool I am by pretending to grow mushroom. I've never done anything involving mycology in my life.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Myyco.com APE Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* A contam question which I cannot find addressed anywhere.... YSJ 4,007 9 02/08/03 04:45 AM
by BigJohnson
* Karo & Neutritional Yeast Contam????? repobob 3,485 8 12/09/02 06:17 AM
by repobob
* Contam or ok? MadHatR 750 1 10/30/03 03:40 PM
by MAIA
* Red Contam ThorA 4,056 9 09/24/01 01:39 PM
by shellacct
* possible contam need help relativexistance 1,006 4 02/13/04 08:43 PM
by Racer6908
* Growing OK but contam on botton of casing. HELP QUILK!!!PICS Birdman 1,063 3 03/02/03 01:31 PM
by Birdman
* is it contam? cinister 1,505 11 07/08/02 09:56 AM
by baraka
* I HATE CONTAM'S!! NSKiller 1,289 7 01/06/03 07:22 PM
by z@z.com

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,384 topic views. 28 members, 101 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.007 seconds on 12 queries.