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Offlinecheech101
most likely alittle hazy

Registered: 05/02/03
Posts: 155
Loc: The Dark Side of the Moon
Last seen: 11 years, 3 months
two questions
    #1517459 - 05/03/03 01:25 PM (13 years, 10 months ago)

when using a spore syringe it is ok to heat the needle with a lighter to sterilize it right before you inculate your jars? I was under the impression that spores were killed by heat.

is any fanning / fresh air needed when jars are colonizing???


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OfflinefIsh in my head
fadedstar

Registered: 01/25/03
Posts: 1,051
Loc: 4500 ASL
Last seen: 24 days, 11 hours
Re: two questions [Re: cheech101]
    #1517467 - 05/03/03 01:33 PM (13 years, 10 months ago)

Well, you will be heating the needle, not the syringe itself. A lighter will do the job. A butane lighter or a alcohol burner is better (for the only reason a damn bic lighter gets really hot after a couple of seconds!) When your needle is sterilized, you must eject a bit of solution out of the syringe just to cool down the needle.  I usually make 4 injections per jar.  I heat the needle and hurry to make my 4 injections.  I guess some people heat the needle after each injection.  I'm not doing it because i'm a cheap ass that doesn't want to screw too much spore solution by heating a filled needle and killing the 0,1 cc left in it and screwing another 0,2 cc for cooling down the needle :laugh:



No fresh air really needed when colonizing.  You jars should be sealed with tape on the holes.  At around 50% colonization, remove tapes and flip jars for a little air exchange.  It will be enough.

good luck!

 


Edited by fIsh in my head (05/03/03 01:36 PM)


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