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Offlinetrophycase
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Tiny Black Specs In Germinating PF Jar... Just Paranoid?
    #15170799 - 10/03/11 08:49 AM (13 years, 3 months ago)

Okay guys, I don't know if I'm just being super paranoid, but I have never noticed this before... These specs are the size of a pinhead. I am praying that they are just spore clumps in the jar. The reason that I think they might be spore clumps is because I found 1 or 2 of these spots stuck to glass where there was no substrate. It is creepin me out because they appear almost exclusively on a single grain or two of BRF when the myc is just starting to germinate. I have a couple jars that are colonizing fairly nicely, and don't seem to have the same black specs. Am I just paranoid?

Btw, I made my own syringe from a print that I have used previously to noc up tons of jars with success. Please let me know what you think:





They are JUST starting to colonize, so it's very hard to see on the camera. You can see some whiter specs of colonizing BRF with the black dots. I'm about to shit my pants over this. Any help would be lovely.


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InvisibleTheDrake
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15170872 - 10/03/11 09:03 AM (13 years, 3 months ago)

looks like verm particles. watch it and make sure it doesnt get any bigger. mold will usually grow very fast.


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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: TheDrake]
    #15170880 - 10/03/11 09:05 AM (13 years, 3 months ago)

:thumbup: That was my plan haha. I think I should be okay because the jars that are farther along were from the same print.

I forgot to mention, but I don't think it's verm particles, because these black dots were only at the inoculation points, and 95% of them are on tiny colonizing grains of BRF.


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InvisibleTheDrake
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15170889 - 10/03/11 09:08 AM (13 years, 3 months ago)

thats possible. how dark did you make your syringes?


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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: TheDrake]
    #15170917 - 10/03/11 09:20 AM (13 years, 3 months ago)

They were fairly cloudy, but from the pictures I have seen of syringes from sponsors, I wouldn't say that these were particularly dark. I guess I will just have to wait it out unless somebody has some experience with this. I'm just wondering if black mold starts out very tiny like this... I have only seen large spots of it.


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OfflineXFINITY
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15171065 - 10/03/11 10:06 AM (13 years, 3 months ago)

You can use a sharpie to mark where they are now, to see if anything changes, if not then you are probably good to go! :thumbup:

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Offlinev33g0237
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: XFINITY]
    #15171084 - 10/03/11 10:11 AM (13 years, 3 months ago)

Yeah and if this is black mold, upon my 1st inoculation with some Oak & BHT's. 2 Oaks had some questionable black specs, but within 10 hours it had spread from a speck to all over the jar and was some nasty evil black mold. Like said above, you'll notice if its mold, especially black with my experience rips all through the substrate pretty quick..


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OfflineXFINITY
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15171121 - 10/03/11 10:23 AM (13 years, 3 months ago)

Quote:

trophycase said:
I'm just wondering if black mold starts out very tiny like this... I have only seen large spots of it.



Everything starts out very tiny, except ostriches they start out rather large (a huge single cell).

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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: XFINITY]
    #15171862 - 10/03/11 01:41 PM (13 years, 3 months ago)

Quote:

XFINITY said:
Quote:

trophycase said:
I'm just wondering if black mold starts out very tiny like this... I have only seen large spots of it.



Everything starts out very tiny, except ostriches they start out rather large (a huge single cell).




Haha. True. I have just never seen pictures of it where it looks this small... These are literally smaller than the tip of a ball-point pen. They just frighten me because of their placement. Only at the inoc points, and MOSTLY in the center of small BRF grains.

Hasn't grown yet, by the way. :shrug:


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InvisibleMunchauzen
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15171986 - 10/03/11 02:05 PM (13 years, 3 months ago)

Any jars you are unsure of, promptly separate them from your good jars to another room. Continue to watch them there so that in case it is contam, it has 0-chance to fuck up your good jars. I call it jar triage, and my questionable ones go in my empty linen closet, away from my walk-in closet where all my good jars are. Once you determine if they are good or not, return them home or do a sterile disposal of the contents.

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InvisibleFungus_monk
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: Munchauzen]
    #15172005 - 10/03/11 02:08 PM (13 years, 3 months ago)

Did you use a lighter or candle to heat the needle?


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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: Fungus_monk]
    #15172104 - 10/03/11 02:31 PM (13 years, 3 months ago)

I use a lighter and then alcohol wipe it before inoculating. I know I should probly have an alcohol lamp and whatnot, but I have done dozens of jars this way without ever noticing these tiny spots.

Edit: I have done dozens of jrs this way with only 1 contam. I would say I'm at least at a 95% success rate.


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Edited by trophycase (10/03/11 02:32 PM)

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InvisibleFungus_monk
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15172113 - 10/03/11 02:34 PM (13 years, 3 months ago)

There was probably soot left on the needle even though you wiped.

I would lose the alcohol wiping though, i mean if it works than it works. No argument against that. But its technically a step back.


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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: Fungus_monk]
    #15172122 - 10/03/11 02:36 PM (13 years, 3 months ago)

Haha. I was HOPING you would say it was soot from the needle. That is a possibility. And the reason I wipe is because of the soot actually... I don't feel right puttin that shit into my jars.


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InvisibleMunchauzen
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase] * 1
    #15172240 - 10/03/11 03:07 PM (13 years, 3 months ago)

RR once said that wiping a needle down with an alcohol pad after flaming it red-hot is like taking a shower then wiping shit all over your face. there is absolutely nothing more sterile than red-hot, so keep that in mind!

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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: Munchauzen]
    #15172291 - 10/03/11 03:18 PM (13 years, 3 months ago)

Idk. IME it's more like taking a shower and then drying off with a day-old towel... It hasn't hurt me yet.


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Offlinev33g0237
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15191344 - 10/07/11 11:55 AM (13 years, 3 months ago)

very interesting points everyone made and I'd like to add something about you not having an alcohol lamp.. I don't either, but didn't want to risk all that bullshit soot, so, if you can find a small glass thing of some sort that will hold alcohol (small glass candle holder), simply pour a bit in it and put a drop on the ledge, light with a lighter, and I always have great success with using this poor mans alcohol lamp or w/e ya wanna call it.. But yea, really no need 2 wipe the needle after it's sterilized red hot unless you see a bunch of soot


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Invisiblemaug
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: v33g0237]
    #15191391 - 10/07/11 12:09 PM (13 years, 3 months ago)

Bacteria is a common contaminate, and it looks a bit like that. It's possible that your LC/spores had bacteria in it. Most of the time the myc will either eat the bacteria or force it into a dormant state. When you spawn or make a new LC it comes back to life. The only time I've seen bacteria win over myc is when there was too much water in the jar.

Just let it do it's thing. Should be fine.


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Offlinetrophycase
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: maug]
    #15194817 - 10/08/11 05:46 AM (13 years, 3 months ago)

Quote:

maug said:
Bacteria is a common contaminate, and it looks a bit like that. It's possible that your LC/spores had bacteria in it. Most of the time the myc will either eat the bacteria or force it into a dormant state. When you spawn or make a new LC it comes back to life. The only time I've seen bacteria win over myc is when there was too much water in the jar.

Just let it do it's thing. Should be fine.




I'm not sure what types of bacteria you were talking about, but my jars look great. I was just being paranoid I guess. I noc'd last friday and one of my jars should be fully colonized by this time tomorrow. :cool:


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Invisiblemaug
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Re: Tiny Black Specs In Germinating PF Jar... Just Paranoid? [Re: trophycase]
    #15195796 - 10/08/11 12:19 PM (13 years, 3 months ago)

I don't know what kind either. I see it occasionally though. I'd rather stick hot coals in my eyes than bring mushrooms to school/lab, so I won't know for a while.


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I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes

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