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shamanamba
The Shaman



Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
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Re-inoculate BRF jars? What would YOU do?
#15170567 - 10/03/11 07:23 AM (13 years, 3 months ago) |
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As some people here may know, I had been struggling with jars not colonizing. On the way to finally figuring out the spore syringes were too old, I ended up producing somewhat of a quantity of BRF jars. Some of the jars contaminated and I dealt with them accordingly, but now I'm left with all these jars which seem perfectly fine but have been sitting up a couple weeks. Think it would be safe to swab an inoculation point with alcohol in the glove box, take of the medical tape and inject some fresh spore solution into these jars?
-------------------- The Shaman
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Doctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc:
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Re: Re-inoculate BRF jars? What would YOU do? [Re: shamanamba]
#15170642 - 10/03/11 07:55 AM (13 years, 3 months ago) |
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If the moisture content of the BRF cakes in the jars with no germination is still acceptable, I'd reinoculate, AFTER another run through the sterilizer to knock back those invader spores that have had a chance to recover from the initial sterilization run.
You're going to have problems with colonization and contamination inoculating regular-mouthed half-pint jars with spores. Culture from spore colonizes better in the half-pint wide-mouthed jars the tek prescribes.
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shamanamba
The Shaman



Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
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Re: Re-inoculate BRF jars? What would YOU do? [Re: Doctor_Inoc]
#15174835 - 10/04/11 12:22 AM (13 years, 3 months ago) |
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hmmmm wide mouth. I don't supposed anyone happen to have a link to a size comparison chart of mason jars? I'm not sure which is which, I have 3 different sizes I've been experimenting with.
anyway I went ahead and put 3 full spore prints into a single syringe to make a super concentrated epicenter of shroominess from which all that is psychedelic in this world emanates, and then knocked up all the jars with the tiniest little fraction of a drop through each inoc hole.
wish I had read the part about pressure cooking them again. Now I feel like an idiot. would have been no trouble at all but i just didn't think about it. well whats done is done and i'll see what happens.
live and learn, I guess
-------------------- The Shaman
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JaffyJaffar
Nom Nom Nom



Registered: 07/26/11
Posts: 1,700
Loc: AMERICA, FUCK YEAH!
Last seen: 12 years, 8 months
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Re: Re-inoculate BRF jars? What would YOU do? [Re: shamanamba]
#15174992 - 10/04/11 01:53 AM (13 years, 3 months ago) |
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I wouldn't knock em back up....mulligan
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dtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
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Re: Re-inoculate BRF jars? What would YOU do? [Re: JaffyJaffar]
#15175021 - 10/04/11 02:13 AM (13 years, 3 months ago) |
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I would use the search function.
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Weller
Loveable rogue


Registered: 05/16/11
Posts: 313
Loc: Spain
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Re: Re-inoculate BRF jars? What would YOU do? [Re: dtowntoker]
#15175310 - 10/04/11 05:30 AM (13 years, 3 months ago) |
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3 spore prints to make 1 syringe is counter productive. Search the site to find out why.
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Big Pappa
Challenged



Registered: 09/03/11
Posts: 717
Loc: Smurf Village
Last seen: 11 years, 17 days
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Re: Re-inoculate BRF jars? What would YOU do? [Re: shamanamba]
#15175483 - 10/04/11 07:14 AM (13 years, 3 months ago) |
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Quote:
shamanamba said: anyway I went ahead and put 3 full spore prints into a single syringe to make a super concentrated epicenter of shroominess from which all that is psychedelic in this world emanates, and then knocked up all the jars with the tiniest little fraction of a drop through each inoc hole.
Were you when you did this????
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We are not human beings having a spiritual experience. We are spiritual beings having a human experience.
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shamanamba
The Shaman



Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
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Re: Re-inoculate BRF jars? What would YOU do? [Re: Big Pappa]
#15177012 - 10/04/11 02:32 PM (13 years, 3 months ago) |
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I did a search and found that too much "spore solution" throws off water content, but everything I've read said that more spores to less solution will speed up colonization.
-------------------- The Shaman
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trophycase
Dickface

Registered: 03/23/11
Posts: 1,018
Last seen: 6 years, 8 months
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Re: Re-inoculate BRF jars? What would YOU do? [Re: shamanamba]
#15177033 - 10/04/11 02:48 PM (13 years, 3 months ago) |
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Water content aint as big a deal as some on here will say. When I was starting out, I didn't do the 2:1:1. I did 2:1 verm to BRF, but never measured water. I just added until field capacity...
Well long story short, I was a noob, and what I thought was field capacity was much much more. Everything turned out fine. Kinda slow colonization, but it worked.
-------------------- All of the cultivation photos uploaded by this account were taken in an area of the globe where such practices are legal and uninhibited.
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Big Pappa
Challenged



Registered: 09/03/11
Posts: 717
Loc: Smurf Village
Last seen: 11 years, 17 days
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Re: Re-inoculate BRF jars? What would YOU do? [Re: trophycase]
#15177324 - 10/04/11 05:14 PM (13 years, 3 months ago) |
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Well, 3 full spore prints could have made a buttload of syringes.... dont matter how many spores you get in there... germination time is all genetics.
Now if you "squirt" spores in way more than 4 points in the jar you can speed up the colonization of those jars, but germination will still take time.
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For Sale
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We are not human beings having a spiritual experience. We are spiritual beings having a human experience.
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