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OfflineAlwaysSunny
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Registered: 10/01/11
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Last seen: 13 years, 3 months
First time grower an first time poster
    #15162079 - 10/01/11 11:32 AM (13 years, 3 months ago)

So I just got interested with growing mushrooms in the last month. Well anyway I ordered 2 half pint PF Tek Mushroom Substrates, Spore syringe of psilocybe B+, and also 1lb bag of perlite and vermiculite. I finally got it last Friday an go right into the inoculation process. So I didn't use a glove box though i probably should of; but instead I used a small bathroom with everything taken out of it and sprayed with a lot of Lysol disinfectant spray and waited about ten minutes. Then I brought in all the supplies and also had to bring a hammer an nail because there were no inoculation holes. Sterilized the hammer and nail and slowly made the holes trying not to touch the vermiculite layer. After that I put tin foil over them for a minute and prepped the syringe by flaming the needle and shaking it vigorously then injected 1/2 CC to each inoculation hole which there were only two. I made sure the needle was touch the outside of glass so the mycelium would grow around the substrate first rather than within. So after all that I put the tin foil over the jars and put them into disinfected shoebox and put on a shelf over my bearded dragons aquarium since it has heat lamps it radiates to the box giving it a nice 80-83 degrees. Then I waited checking everyday with sterilized precautions such as rubber gloves and loads of disinfectant spray.

Now it's been a week and when I open the shoebox it kinda smells a bit milky. Not necessarily rancid milk but close to curdled. I don't see an contamination but I could be wrong since Im new to this. There are a few white spots. So I'm not sure if I should throw this jars out an start again or just wait?

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Offlineamberella1989
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Registered: 08/19/11
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Re: First time grower an first time poster [Re: AlwaysSunny]
    #15162091 - 10/01/11 11:35 AM (13 years, 3 months ago)

I'd wait and see. no need to be so cautious either when your looking at them.


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OfflineMathna
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Loc: Stormwind, Eastern Kingdo...
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Re: First time grower an first time poster [Re: amberella1989]
    #15162100 - 10/01/11 11:36 AM (13 years, 3 months ago)

Did you use a pressure cooker?


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-<<***Alacabenzi mInI mOnO***>>-


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OfflineJaffyJaffar
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Registered: 07/26/11
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Re: First time grower an first time poster [Re: AlwaysSunny]
    #15162111 - 10/01/11 11:39 AM (13 years, 3 months ago)

that smell means contamination more than likely

80-83 is to warm for colonization

plus when there's foil over the lids it hinders gas exchange which is what is needed for colonization

i see other possible flaws and recommend the let's grow vids to a t and make your own jars next time

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OfflineXeR0
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Registered: 11/13/10
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Re: First time grower an first time poster [Re: Mathna]
    #15162118 - 10/01/11 11:41 AM (13 years, 3 months ago)

You should be just fine. As long as you followed the PF Tek as per the mushroomvideos website, you should do just fine. To be honest, the whole care about contamination is more or less a safety precaution. You don't have to be THAT cautious unless your area is prone to fits of mold and whatnot. The most I've done besides sterilizing the jars and using alcohol is flame sterilizing the needle and using gloves. Other than that, I think a glovebox is a little too much for me. I'm on my second grow and I haven't had a contam yet.

Good luck on your first grow! It is quite a rewarding experience...


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Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

ToDo: Galantamine, Hydergine

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OfflineEburger
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Re: First time grower an first time poster [Re: AlwaysSunny]
    #15162139 - 10/01/11 11:47 AM (13 years, 3 months ago)

You probably should have Pressure cooked or boiled those jars before inoculation.  Also, if your jars have a verm. barrier, you should take the foil off of the tops of the jars for Fresh air exchange.  You will probably be disappointed with your results.  You can easily make your own PF jars.  2/3 cup Verm, 1/4 cup water & 1/4 brown rice flour (ground organic brown rice)

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OfflineJaffyJaffar
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Registered: 07/26/11
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Re: First time grower an first time poster [Re: Eburger]
    #15162149 - 10/01/11 11:50 AM (13 years, 3 months ago)

people...it sounds like he ordered the pre-made sterilized jars

quote:

          I ordered 2 half pint PF Tek Mushroom Substrates


then he continues to say he had to nail holes because they didn't come with holes

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Offlinetripdawg420
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Re: First time grower an first time poster [Re: JaffyJaffar]
    #15162163 - 10/01/11 11:55 AM (13 years, 3 months ago)

:ugh:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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OfflineMathna
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Registered: 01/07/10
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Loc: Stormwind, Eastern Kingdo...
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Re: First time grower an first time poster [Re: tripdawg420]
    #15162198 - 10/01/11 12:05 PM (13 years, 3 months ago)

I think I found what you ordered and it does say you need to make your own innoc points. Very interesting to me O.o 

http://everythingmushrooms.com/grow-your-own-mushrooms/sterilized-substrates-and-media/pf-tek-mushroom-cake-substrate-jars-half-pint-wide-mouth-sterilized/


--------------------
-<<***Alacabenzi mInI mOnO***>>-


Edited by Mathna (10/01/11 12:07 PM)

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OfflineThe Influence
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Registered: 03/30/11
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Re: First time grower an first time poster [Re: Mathna]
    #15162332 - 10/01/11 12:52 PM (13 years, 3 months ago)

Put them out on an open shelf to colonize. If you see white spots, thats most likely mycelium. Pics would help.

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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
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Re: First time grower an first time poster [Re: The Influence]
    #15162336 - 10/01/11 12:53 PM (13 years, 3 months ago)

how would you make holes with out distubing the dry verm layer :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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OfflineThe Influence
Free Sheeks
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Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 1 day
Re: First time grower an first time poster [Re: tripdawg420]
    #15162351 - 10/01/11 12:59 PM (13 years, 3 months ago)

Quote:

tripdawg420 said:
how would you make holes with out distubing the dry verm layer :shrug:



Very carefully...like a ninja.

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OfflineAlwaysSunny
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Registered: 10/01/11
Posts: 4
Last seen: 13 years, 3 months
Re: First time grower an first time poster [Re: Mathna]
    #15164897 - 10/01/11 11:36 PM (13 years, 3 months ago)

No, I didn't use a pressure cooker because I thought the jars were already sterilized in one before being shipped. So says this link http://everythingmushrooms.com/grow-your-own-mushrooms/pf-tek-mushroom-cake-substrate-jars-half-pint-regular-mouth-sterilized/

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OfflineBig Pappa
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Registered: 09/03/11
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Re: First time grower an first time poster [Re: AlwaysSunny]
    #15164993 - 10/02/11 12:18 AM (13 years, 3 months ago)

Good luck with that. 

How did you "sterilize" the nail and hammer???? 

I see so many things you did that COULD be very very bad...  but you may get lucky.  Remove the foil, let them breathe.....


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OfflineAlwaysSunny
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Registered: 10/01/11
Posts: 4
Last seen: 13 years, 3 months
Re: First time grower an first time poster [Re: Big Pappa]
    #15168901 - 10/02/11 08:18 PM (13 years, 3 months ago)

Yeah, I sprayed the hammer with disinfectant spray and put the nail in rubbing alcohol. Now, I've taken the jars out of the box and removed the tin foil and replaced it with a coffee filter then left the two jars on a shelf.

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Offlineigigi
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Registered: 09/10/11
Posts: 154
Loc: Sumer, Mesopotamia
Last seen: 5 years, 3 months
Re: First time grower an first time poster [Re: AlwaysSunny]
    #15168937 - 10/02/11 08:27 PM (13 years, 3 months ago)

Quote:

RogerRabbit said:
Coffee filters are for brewing coffee. Trying to catch contaminants with a coffee filter is like trying to catch minnows with a deep sea fishing net.  If you're going to inoculate through tyvek, cover the hole with micropore tape.
RR




For your later grows.. foil for the pressure cook and micropore the hole(s) after noc'ing them up.


Also, if/when you have/get a pressure cooker, your contam rate will shoot waaay down.  Learn to how to use it properly, it'll serve you very well.  True the verm layer *should* be enough to keep contams out, but better SAFE than sorry.


--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic.  Click and READ the first link.  I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/

Edited by igigi (10/02/11 08:28 PM)

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OfflineAlwaysSunny
Stranger
Registered: 10/01/11
Posts: 4
Last seen: 13 years, 3 months
Re: First time grower an first time poster [Re: igigi]
    #15169028 - 10/02/11 08:50 PM (13 years, 3 months ago)

I see, thanks for the heads up. Wasn't too sure where to get micropore, possibly a local pharmacy or something.

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OfflineXeR0
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Registered: 11/13/10
Posts: 409
Last seen: 4 years, 5 months
Re: First time grower an first time poster [Re: AlwaysSunny]
    #15169063 - 10/02/11 08:59 PM (13 years, 3 months ago)

Micropore tape can be found at almost ANY local pharmacy. It definitely is worth buying.


--------------------
Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

ToDo: Galantamine, Hydergine

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Offlineigigi
Male


Registered: 09/10/11
Posts: 154
Loc: Sumer, Mesopotamia
Last seen: 5 years, 3 months
Re: First time grower an first time poster [Re: XeR0]
    #15169095 - 10/02/11 09:07 PM (13 years, 3 months ago)

I definitely agree, micropore is very nice to have around!
I got hosed on the price through walgreens.. check local mexican food stores, I see cheap medical supplies at them frequently.


--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic.  Click and READ the first link.  I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/

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