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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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Re: Lemme see your GloveBox [Re: Big Pappa]
#15167588 - 10/02/11 03:17 PM (13 years, 3 months ago) |
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you really dont want moving air in a sagb
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Lemme see your GloveBox [Re: tripdawg420]
#15167597 - 10/02/11 03:19 PM (13 years, 3 months ago) |
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Quote:
tripdawg420 said: you really dont want moving air in a sagb 
QFT
You also don't want to attach the gloves, as you won't be able to flame your needle/scalpel outside the SAB.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Lord_McLovin
mad scientist on shrooms



Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 7 years, 25 days
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Re: Lemme see your GloveBox [Re: Big Pappa]
#15167611 - 10/02/11 03:23 PM (13 years, 3 months ago) |
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Quote:
Big Pappa said: Yes, it draws in air from the room through the HEPA and into the box creating a positive pressure in the box. It then blows out the arm holes.
Works real good, but I keep getting contams on agar plates. It MAY be that I havent been wrapping tape around them when letting them cool though.... but I do leave the HEPA running. I have not had ANY contams get into any of my jars....
The reason you're getting contams on agar is that you create a turbulent flow of air within your glove box. The concept does not work.
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PeterPanda209
Who has BitCoins?



Registered: 06/29/11
Posts: 6,016
Loc: •FrEsH CoAsT
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QFT?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quoted for Truth
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 7 hours, 42 minutes
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Re: Lemme see your GloveBox [Re: tripdawg420]
#15167722 - 10/02/11 03:51 PM (13 years, 3 months ago) |
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My gloves are attached and I flame inside my glovebox.....
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FuckMeRunnin
REALLY good looking


Registered: 07/18/09
Posts: 788
Last seen: 11 years, 7 months
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Yeah, I just use a box with two holes cut into it...It really doesn't have to be all extreme. The main concept is to keep the air inside still. I realize some people like to spend time and money to make things over the top. Also is a good way to take up the time while waiting for your mushies to grow.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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i have a 4ft by 4ft by 6.6ft still air room i use a clear plastic shower curtain for the door with my still air glove box in the growbox it was going to be a weed grow room but growing weed seem hard as fuck
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 7,373
Loc: In the Gills
Last seen: 7 hours, 42 minutes
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Quote:
FuckMeRunnin said: Yeah, I just use a box with two holes cut into it...It really doesn't have to be all extreme. The main concept is to keep the air inside still. I realize some people like to spend time and money to make things over the top. Also is a good way to take up the time while waiting for your mushies to grow.
I agree I use to just use the two holes cut but i decided to attach
yellow cleaning gloves. I'm glad I did it works good and I have very low
contams
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PeterPanda209
Who has BitCoins?



Registered: 06/29/11
Posts: 6,016
Loc: •FrEsH CoAsT
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Re: Lemme see your GloveBox [Re: rumfor69]
#15167783 - 10/02/11 04:06 PM (13 years, 3 months ago) |
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So along with a tote, some tyvek, gloves... What else is essential? Some sort of alcohol Id guess? Though the air is still how would you "sterilize" it? Just spray some bleach water mixture or?
Also you know those alcohol lamp looking things people use? What are they called I wanna find a one or a tek for a home made one
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FuckMeRunnin
REALLY good looking


Registered: 07/18/09
Posts: 788
Last seen: 11 years, 7 months
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Re: Lemme see your GloveBox [Re: rumfor69]
#15167793 - 10/02/11 04:08 PM (13 years, 3 months ago) |
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With my 2 hole box, I can do needle work, clone to lc, G2G with very high success rates. Only thing that I haven't had the best results with is agar work. I attribute it more to how I made my jars, for some reason I added holes in the top and covered with just micropore tape. When I use just micropore tape as a filter for anything I never have good results. That being said, I can't say it's just the GB that is responsible for my higher then average failure rates from agar. I'm definitely going to be building a flow hood soon, have the furnace fan, the materials to make the inclosure, just need to buy a filter. Then good bye GB.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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i wash it with dish soap and water and wipe it out with a lysol wipe and spray lysol put the lid on spray all around the room
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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They're called alcohol lamps.
You can just take a shoe lace, drill a small hole in the lid of a jar & fill the jar w/ denatured alcohol. Instant alcohol lamp.
everythingmushrooms.com has an alcohol lamp on it.
Bleach+water is fine for cleaning. Even just warm water + soap is fine. Make sure the SAB is damp when working inside it.
Here's a pic of my alcohol lamp (store bought)
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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fuck ya
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PeterPanda209
Who has BitCoins?



Registered: 06/29/11
Posts: 6,016
Loc: •FrEsH CoAsT
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Re: Lemme see your GloveBox [Re: tripdawg420]
#15167849 - 10/02/11 04:24 PM (13 years, 3 months ago) |
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Lol well I feel stupid for over examining and complicating it. Thanks alot man, so what's the purpose of keeping the SAB damp?
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FuckMeRunnin
REALLY good looking


Registered: 07/18/09
Posts: 788
Last seen: 11 years, 7 months
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Re: Lemme see your GloveBox [Re: tripdawg420]
#15167858 - 10/02/11 04:27 PM (13 years, 3 months ago) |
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Yeah my alcohol lamp is pretty ghetto just like my GB Its just a tall wide mouth half pint jar, with a hole punched in the lid with cotton balls shaped into a wick that dangle into the rubbing alcohol I fill the jar with. Works awesome doesn't go out and I'v been using the same cotton wick for 2 years hahaha.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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so with the lil turbelance you make in the sagb it makes it hard to blow around
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
PeterPanda209 said: Lol well I feel stupid for over examining and complicating it. Thanks alot man, so what's the purpose of keeping the SAB damp?
my design is a little over the top.
Any contams that fall will get stuck to the damp bottom or walls of the SAB.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 17 hours, 32 minutes
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