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Offline760Rollz
Out the game for now. Peace all
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Registered: 03/01/10
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Growth seems to have halted
    #15164514 - 10/01/11 09:36 PM (13 years, 3 months ago)

I've inoculated four BRF cakes. Each cake was made to the exact ratios RR specifies in his video.  I used the short fat half pint wide mouth jars. I've seen growth on one of the 4 inoculated jars.  Since the first signs of growth I've watched the it very closely.  At first the jar seemed to take off with what seemed to be good strong mycelium.  It seemed to double in size each and every day over the course of about three days.  But now it seems to have grown to a crawl, if not a total halt.  I read that I only needed to inject each jar with 2cc solution, so I injected one of the holes with a good 2ccs and then began watching for growth. 

Here are a few pictures to show the growth over the last few days.  The first pic was taken five days ago.  Then the second pic was taken 2 day ago and the 3rd pic was taken tonight.  I hope you can tell how much the mycelium in the jar has or has not grown from the pictures posted.  Is this normal for things to move this slow or does it look like the mycelium might have died? 


Thanks for looking,
Thanks to those who take the time to give their input,

J


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Invisibledtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
Re: Growth seems to have halted [Re: 760Rollz]
    #15164521 - 10/01/11 09:39 PM (13 years, 3 months ago)

2mL total, not per hole.  That is .5mL per hole.


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Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
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Re: Growth seems to have halted [Re: dtowntoker]
    #15164539 - 10/01/11 09:44 PM (13 years, 3 months ago)

:whathesaid:

But even less than that is acceptable. But growth in only one jar? Did you shake the syringe prior to use?

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Offline760Rollz
Out the game for now. Peace all
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Registered: 03/01/10
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Re: Growth seems to have halted [Re: dtowntoker]
    #15164547 - 10/01/11 09:46 PM (13 years, 3 months ago)

Quote:

dtowntoker said:
2mL total, not per hole.  That is .5mL per hole.


Wow that is such a small amount per hole.  As you can see I did get growth on the one hole I hit.  Do you think that shooting too much into the one hole might be the reason growth seems to have stopped?  Am I just expecting too much too fast?  There is absolutely no signs of contams, but it seems to be stagnant and not covered any more space in the past two days.


Quote:

wildernessjunkie said:
:whathesaid:

But even less than that is acceptable. But growth in only one jar? Did you shake the syringe prior to use?


Yup I shook it all up.  I did keep the syringe in the fridge a day or two before I used it, and I didn't use, "sex lube" with it as RR says to do if you are going to store the syringe before using it.  But I hadn't seen RR's full length video yet.  I had only watched the clips you can find on youtube prior to my first inoculation.  But yes I did shake the needle.

Edited by 760Rollz (10/01/11 09:53 PM)

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Invisiblewildernessjunkie
Reshitivest
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Posts: 8,118
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Re: Growth seems to have halted [Re: 760Rollz]
    #15164609 - 10/01/11 10:00 PM (13 years, 3 months ago)

I would keep waiting then. Ive had spores take 3 weeks to germinate, sometimes it just takes time. As long as it doesn't turn green, then I would just be patient. Not much more you can do.

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Offline760Rollz
Out the game for now. Peace all
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Registered: 03/01/10
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Re: Growth seems to have halted [Re: wildernessjunkie]
    #15164642 - 10/01/11 10:10 PM (13 years, 3 months ago)

^^^:crazy:That's a long time:crazy:^^^   
Wait it shall be.  I'm just afraid that it is not growing at all any more.  Hopefully I'm just paranoid and impatient.


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OfflineContent
Hi
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Registered: 03/31/10
Posts: 1,241
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Re: Growth seems to have halted [Re: 760Rollz]
    #15165015 - 10/02/11 12:28 AM (13 years, 3 months ago)

Could be the spores. My first experiment I received a syringe that didn't germinate well and took 11 days to see first signs of growth in only a few jars. Once I gathered my own fresh spores and made my own syringe, I now get steady, fast growth in ALL my jars. I even see signs of myc in 2 days. Just try to be patient and wait for more growth. It's hard I know... trust me I KNOW.


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Improvements sha'll be made.

PESHawaiian ~ 4-HO-DMT

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