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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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wildlifeonmars
Caveman Scientist
Registered: 10/05/11
Posts: 12
Loc: Cow Pie, USA
Last seen: 5 years, 6 months
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: Icesyn]
#15286346 - 10/27/11 02:07 PM (12 years, 4 months ago) |
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Thanks for the writeup! Very helpful, especially the drying of the grains, I always end up making a big mess, then the wife gets PO'd Thank you! -WLOM
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IamYou
heretic
Registered: 10/11/11
Posts: 704
Last seen: 7 years, 6 months
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15286389 - 10/27/11 02:15 PM (12 years, 4 months ago) |
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Quote:
EvilMushroom666 said:
Quote:
IamYou said: pre-sealable.5 micron filter patch bags
i would be worried about small spores coming in through a 5 micron hole.
I know they do in fact have .5 micron bags, but here is the link to the manufacturer that most vendors get their bags from. As you can see the B filter patch is rated at 5 micron, not .5.
http://unicornbags.com/store/product_info.php/cPath/26/products_id/33/osCsid/ab718e00c197e9a09792a925c0b53b19
Perhaps that is a typo on their part. I know the 14A filter patch bags are rated at .5 micron
http://unicornbags.com/store/product_info.php/cPath/26/products_id/32
hmm... i hope thats not the case. well, the bags you linked were 8" X 4.8" X 19" and the vendor i am goin thru advertises 8" x 5" x 18". which is really close to the same thing and im hoping that this filter is actually a 0.5 instead of 5 micron.
i think generally cube spores are around 15-25 microns??? something like that maybe? but just in case those bags do have a 5 micron filter, would you feel comfortable about those keeping out most contams? they will have rye in them fyi.
-------------------- “Life lived in the absence of the psychedelic experience is life trivialized, life denied, life enslaved to the ego.” - You Remember, be here now...
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: IamYou]
#15286587 - 10/27/11 02:52 PM (12 years, 4 months ago) |
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Search the forums for more information on the 5 micron filters. Roger Rabbit hates them and says that when he uses them for grain or sawdust blocks a spotch of trichoderma usually develops right below the filter patch.
I have used the 5 micron bags for supplemented sawdust blocks in the past without issues, but notice I have used may be 5 for shiitake blocks, RR makes around 20 per day if not more...so you can see how he would have a better look at the probability of things happening.
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IamYou
heretic
Registered: 10/11/11
Posts: 704
Last seen: 7 years, 6 months
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15287004 - 10/27/11 04:16 PM (12 years, 4 months ago) |
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right on, hopefully he might run across this thread and chime in if he knows if these bags are the 5 micron POS's or not...
sorry for being off topic but gratz on not smokin anymore i managed to quit smokin myself. its been about 3 months for me i think.
-------------------- “Life lived in the absence of the psychedelic experience is life trivialized, life denied, life enslaved to the ego.” - You Remember, be here now...
Edited by IamYou (10/27/11 04:19 PM)
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dtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: IamYou]
#15287056 - 10/27/11 04:27 PM (12 years, 4 months ago) |
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A little micropore over the filter would help that, no?
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IamYou
heretic
Registered: 10/11/11
Posts: 704
Last seen: 7 years, 6 months
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: dtowntoker]
#15287155 - 10/27/11 04:49 PM (12 years, 4 months ago) |
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hmm.. do you know what kind of rating micropore tape has?
-------------------- “Life lived in the absence of the psychedelic experience is life trivialized, life denied, life enslaved to the ego.” - You Remember, be here now...
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Doctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc:
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: IamYou]
#15288457 - 10/27/11 09:17 PM (12 years, 4 months ago) |
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Quote:
IamYou said: Anybody have any exp with the RTV on filter patch bags? i was planning on just applying it to one side of the bags but what do you guys/gals think?
As Evil said, scuff the surface of where the injection port is to be placed with a piece of sand-paper. The grooves that the sand-paper create causes the RTV to adhere good.
Used to install RTV injection ports to the backs (opposite side of the filter-patch) of bags before loading them, allowing 24 hours for the RTV to dry and set before PC'ing.
RTV injection ports for spawn bags are a good idea when you're inoculating with a syringe solution and no flowhood. Just flame sterilize the needle, inject through port, and inoculate.
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: IamYou]
#15290827 - 10/28/11 12:48 PM (12 years, 4 months ago) |
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Quote:
IamYou said: sorry for being off topic but gratz on not smokin anymore i managed to quit smokin myself. its been about 3 months for me i think.
Its the best thing you can do for your health I tell yah. Here is a good tool to stay motivated:
http://www.endthehabit.com/content/calculator.htm
Quote:
I have now stopped smoking for 1 year, 4 months, 3 weeks, 3 days, 5 hours, 47 minutes, 3 seconds. That translates into 12,856 cigarettes NOT smoked, for a savings of $4,885.29! At 5 minutes per cigarette I have increased my life expectancy by 1 month, 2 weeks, 2 days, 15 hours, 20 minutes, 7 seconds.
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15476117 - 12/06/11 05:50 PM (12 years, 3 months ago) |
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 21,627
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15477334 - 12/06/11 09:38 PM (12 years, 3 months ago) |
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Gotta love it... Got a little somethin' extra in store for ya EM
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce How I Do EVERYTHING "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: TranscendingLife]
#15670495 - 01/15/12 04:31 PM (12 years, 2 months ago) |
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15812774 - 02/15/12 09:57 AM (12 years, 1 month ago) |
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After answering a few grain spawn questions today I figured this may serve some good back on the front page.
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billy
Registered: 07/13/11
Posts: 554
Last seen: 6 years, 20 days
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15822621 - 02/17/12 09:22 AM (12 years, 1 month ago) |
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i followed this tek for my first real attempt at grain.(i made 2 half ass attempts with no instruction and failed) thank you EM666 for the great tek! 908g wet(1/2 of first flush)
golden teacher multispore 1 to 2 spawn to sub/ratio
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: billy]
#15824461 - 02/17/12 05:56 PM (12 years, 1 month ago) |
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Stellar work brother, thank you for sharing your success with us all!
Some beautiful fruits you have there.
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BotanyPhD
Botanist
Registered: 03/12/12
Posts: 2
Loc: Southern US
Last seen: 9 years, 5 months
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#15996914 - 03/25/12 11:50 PM (11 years, 11 months ago) |
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Another way of injection ports on bags, what I use is cheap waterproof band aids. Place that on the bag before you pc. You can shoot through the center of the bandaid and it basically self heals. Been using it for years in tissue culture as an injection port for binary media. You can pick up some up at the dollar store. The waterproof ones will survive the autoclave a few times.
-------------------- “Anyone who acquires more than the usual amount of knowledge concerning a subject is bound to leave it as his contribution to the knowledge of the world.”
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afungi
Registered: 11/10/12
Posts: 47
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#17252422 - 11/20/12 12:50 PM (11 years, 3 months ago) |
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Just wanted to thank you for the Tek.
I just finished doing my first batch of jars for Shiitake and Oysters, and it looks real good.
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: afungi]
#17253455 - 11/20/12 04:06 PM (11 years, 3 months ago) |
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Looks good, wish you good luck with your grow!
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Mr. Alien
I will abduct andprobe your anus
Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: EvilMushroom666]
#19821557 - 04/10/14 12:36 AM (9 years, 11 months ago) |
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Hello i have several questions
Why to seal the bag with the tyvek sleeve method if you are going to open the bag again to put the wire?
Is it because you have to inflate the bag with air then seal it with the wire? and if so why not to inflate and seal the bag with the wire from the beginning before sterilization?
Or he did the Tyvek Sleeve method because he was going to inoculate the bag later with G2G transfer?
If I'm going to inoculate a bag with spore solution, Am i going to still have to do the tyvek sleeve method? Or just seal the bag from the beginning and leave the air space and sterilize..?
Edited by Mr. Alien (04/10/14 12:59 AM)
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Masticore
Perpetually Curious
Registered: 12/16/09
Posts: 1,159
Last seen: 6 years, 11 months
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: Mr. Alien]
#19832268 - 04/12/14 11:23 AM (9 years, 11 months ago) |
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If you're really worried about contamination, leave the bag sealed and go about inoculation a different way. This tek is done in front of a flow hood, and not as risky as open air or a makeshift glove box.
Most people I've seen use the tyvec sleeve in the bag so they can inoculate with G2G transfer. Spores to grain bags is totally something you can do though.
-------------------- Treat my posts like the Bible. I am fallible, subject to human limitation, and usually open to interpretation.
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Mr. Alien
I will abduct andprobe your anus
Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: How EvilMushroom666 Prepares Grain Spawn (Jars & Bags) [Re: Masticore]
#19832606 - 04/12/14 12:37 PM (9 years, 11 months ago) |
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Thanks for the reply
If i leave air in the bag and seal and then sterilize, the high pressure in the sterilization process would take out the air from the bag through the filter?
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