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Kumara
Dragoness


Registered: 02/24/09
Posts: 179
Last seen: 2 months, 20 days
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Need help with Pan Cyan germination
#15158063 - 09/30/11 01:11 PM (13 years, 3 months ago) |
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I've never grown pan cyans before (although I've tried).
My syringe is from a sponsor, purchased within the past several weeks.
First attempt - noc'd up 4 half-pint rye/coffee/gypsum jars (to make mycelium water - I'm very comfortable and familiar with the process). No growth after 1-1/2 weeks.
I also noc'd up 4 half-pint jars of WBS/coffee/gypsum soon after, and also no growth.
I tried growing p. cyans a few years ago, when I first started growing, and failed miserably. I noc'd up a bunch of LC's (syringe was from the same sponsor), and NONE of them showed even the faintest bit of growth. I gave up and focused on other species and wanted to give them another try now that I have more experience.
The next thing I think I could try is to put it on agar, but I really hate to do that from a syringe. I will if I don't get any better advice.
I am also considering making wbs/coffee/worm castings/gypsum jars, wondering if the worm castings will entice the spores to do their thang.
Any suggestions?
Thank you!
Edited by Kumara (09/30/11 05:54 PM)
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MushroomHaven
Capi-Tan



Registered: 05/06/09
Posts: 782
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Re: Need help with Pan Cyan germination [Re: Kumara]
#15165689 - 10/02/11 07:52 AM (13 years, 3 months ago) |
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I saw in a thread that someone was making brf jars but making the ratio 1:1:1:1, adding hpoo and gypsum to the mix. They said they had full colonization of half pint jars at 19 days with cyans. Maybe give this a try, If I find the thread I will post the link.
-------------------- If someone offers you 10k to fly to a foreign country and bring back a suitcase, do yourself a favor and walk away..
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MushroomHaven
Capi-Tan



Registered: 05/06/09
Posts: 782
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Re: Need help with Pan Cyan germination [Re: MushroomHaven]
#15165698 - 10/02/11 07:55 AM (13 years, 3 months ago) |
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Here it is, and also no gypsum, dont know what I was thinking, it is one part water, one part poo, one part verm, one part brf. Heres the link: http://www.shroomery.org/forums/showflat.php/Number/12165374#12165374
Hope it helps and GL
-------------------- If someone offers you 10k to fly to a foreign country and bring back a suitcase, do yourself a favor and walk away..
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XFINITY
Not a noob just quiet



Registered: 03/14/10
Posts: 676
Loc: 33*44'43.5N 117*55'08.2W
Last seen: 1 year, 9 months
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Re: Need help with Pan Cyan germination [Re: MushroomHaven]
#15165716 - 10/02/11 08:05 AM (13 years, 3 months ago) |
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Wow that is crazy I got some Pan Cyans from Sporeworks and my quart jars of wheat spawn/gypsum was fully colonized in 10 days from mulitspore. You may have just gotten a bad syringe. In all honesty the Pan Cyans Hawaiian has been the most aggressive colonizer I have witnessed of any psilocybin containing mushroom. Blowing by even my fastest cubensis strains. They are in trays of manure and straw now and it has been 5 days and they are almost ready to case. Keep us posted and PM me in a couple weeks for a (proven) specimen print.
Edited by XFINITY (10/02/11 08:06 AM)
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: Need help with Pan Cyan germination [Re: MushroomHaven]
#15165730 - 10/02/11 08:09 AM (13 years, 3 months ago) |
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Quote:
dont know what I was thinking, it is one part water, one part poo

The term is manure. We're all supposed to be adults here. RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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XFINITY
Not a noob just quiet



Registered: 03/14/10
Posts: 676
Loc: 33*44'43.5N 117*55'08.2W
Last seen: 1 year, 9 months
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Re: Need help with Pan Cyan germination [Re: RogerRabbit]
#15165849 - 10/02/11 08:43 AM (13 years, 3 months ago) |
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Quote:
RogerRabbit said:
Quote:
dont know what I was thinking, it is one part water, one part poo

The term is manure. We're all supposed to be adults here. RR
EWW speaking of, I wonder if anyone has every tired to use their own...manure...
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Kumara
Dragoness


Registered: 02/24/09
Posts: 179
Last seen: 2 months, 20 days
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Re: Need help with Pan Cyan germination [Re: RogerRabbit]
#15165866 - 10/02/11 08:48 AM (13 years, 3 months ago) |
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Thanks so much for the feedback! I made some rye jars with worm castings, and if those don't take off, I'll try a brf/manure-poo jar. If those don't do anything I won't bother with agar and I won't invest any more time in this particular syringe.
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XFINITY
Not a noob just quiet



Registered: 03/14/10
Posts: 676
Loc: 33*44'43.5N 117*55'08.2W
Last seen: 1 year, 9 months
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Re: Need help with Pan Cyan germination [Re: Kumara]
#15165926 - 10/02/11 09:05 AM (13 years, 3 months ago) |
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Why not just try the rye by itself? Or test with a couple of agar plates before taking so much time and energy out to make grain spawn? If I were you I would contact the vender first and request a replacement. Just say something like, "The spores I received don't appear too viable under the microscope."
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MushroomHaven
Capi-Tan



Registered: 05/06/09
Posts: 782
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Re: Need help with Pan Cyan germination [Re: RogerRabbit]
#15165973 - 10/02/11 09:17 AM (13 years, 3 months ago) |
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Quote:
RogerRabbit said:
Quote:
dont know what I was thinking, it is one part water, one part poo

The term is manure. We're all supposed to be adults here. RR
My bad on the poo, just quicker to type than manure, you know us "kids" always in a hurry to do nothin.
-------------------- If someone offers you 10k to fly to a foreign country and bring back a suitcase, do yourself a favor and walk away..
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Kumara
Dragoness


Registered: 02/24/09
Posts: 179
Last seen: 2 months, 20 days
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Re: Need help with Pan Cyan germination [Re: XFINITY]
#15166144 - 10/02/11 10:03 AM (13 years, 3 months ago) |
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Quote:
XFINITY said: Why not just try the rye by itself? Or test with a couple of agar plates before taking so much time and energy out to make grain spawn? If I were you I would contact the vender first and request a replacement. Just say something like, "The spores I received don't appear too viable under the microscope."
I'm not sure I want another print or syringe from this vendor of this strain, considering this is the second one I've purchased with no germination.
I've already tried the rye by itself (see OP). When working with something from multispore, I make up a few half-pint jars of wbs or rye. Once those colonize, I use the mycelium from those to inoculate quart jars or to put on agar or make BRF jars, depending on what my current project goals are.
I don't like to put anything from a syringe onto agar, because often any bacteria in the water is going to overrun the plate before the spores even germinate. If I do manage to get some myc, because working with agar is so challenging for me due to my current inadequate setup, it's not worth it to sample it and grow out more plates for this particular project. It's easier to get a different print from a different source - if that fails then I can reasonably ascertain it's me and then go from there.
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Kumara
Dragoness


Registered: 02/24/09
Posts: 179
Last seen: 2 months, 20 days
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Re: Need help with Pan Cyan germination [Re: Kumara]
#15173099 - 10/03/11 06:19 PM (13 years, 3 months ago) |
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It just occurred to me that my syringe was probably exposed to +100 degree F temps en route. That would affect viability, yes?
If that is the case, would a spore print better survive the heat?
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Kumara
Dragoness


Registered: 02/24/09
Posts: 179
Last seen: 2 months, 20 days
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Re: Need help with Pan Cyan germination [Re: Kumara]
#15197067 - 10/08/11 05:50 PM (13 years, 3 months ago) |
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Update: Two LME LC's - fail. Four jars rye/worm castings - 2 are fail, 2 show tiny spots of myc, woot!
Update 12/02/2011:
The 2 jars showing growth colonized. I made myc water with one of them and inoculated 20 jars PF Tek style, using verm/cow manure/brf mixture.
The jar I made the myc water from re-colonized SUPER fast, and the growth looks much more robust and healthy than before. This has also been my experience with cubensis, I'm thinking that the myc likes more moisture, so long as it isn't pooling on the bottom of the jar.
The myc water also colonized into a big fluffy cloud of myc, I wondered if it would since the worm castings transferred from the rye jar to the jar of water. Good growth on my PF tek jars so far... it's sinking in that I may actually have some pans on my hands in a few weeks.
Edited by Kumara (12/02/11 07:16 PM)
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