Home | Community | Message Board


Shroom Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag   Amazon ½ Pint Jars, Vermiculite

Jump to first unread post. Pages: 1
Offlineslothrop
Stranger
Registered: 09/30/11
Posts: 3
Last seen: 9 years, 4 months
The whole syringe got sucked into one jar...
    #15157610 - 09/30/11 01:41 PM (9 years, 7 months ago)

Following the PF tek, something went wrong on the fourth jar. Once the needle was stuck in the inoculation hole, the entire contents of the syringe were sucked into the jar without me applying any pressure at all. It happened very quickly.

Has anyone else run into this problem? Obviously there was some suction in the jar - what can I do to avoid this in the future? Puncture a hole with a sterile needle or something first? I was using masking tape.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinejimmyjame1
Lover
 User Gallery


Registered: 03/13/10
Posts: 2,076
Loc: Flag
Last seen: 2 years, 3 months
Re: The whole syringe got sucked into one jar... [Re: slothrop]
    #15157657 - 09/30/11 01:52 PM (9 years, 7 months ago)

thats really weird man.. never heard of that before


--------------------
:nyan:Link to My Journaland My Trade List :nyan:


LOVE PEACE AND BEAUTIFUL MUSIC.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleFooManM
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,955
Loc: Earth Flag
Trusted Cultivator
Re: The whole syringe got sucked into one jar... [Re: slothrop]
    #15157675 - 09/30/11 01:56 PM (9 years, 7 months ago)

Drill your holes a little wider. I've never had that happen, but I'm assuming the tape was really doing it's job and created an airtight seal and a vacuum formed when the jar cooled after cooking, you put the needle into the just-big-enough hole and the vacuum of the jar sucked out the contents of your syringe.

Did you inject through the tape?


--------------------

Quick WBS Prep


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 7 years, 8 months
Re: The whole syringe got sucked into one jar... [Re: jimmyjame1]
    #15157676 - 09/30/11 01:56 PM (9 years, 7 months ago)

It sounds like there was a vacuum in the jar, were the inoculation points covered in silicone or something?


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleFooManM
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,955
Loc: Earth Flag
Trusted Cultivator
Re: The whole syringe got sucked into one jar... [Re: FooMan]
    #15157689 - 09/30/11 01:59 PM (9 years, 7 months ago)

Also, you're you should leave the lids a bit loose during cooking so that a vacuum doesn't form in the first place.


--------------------

Quick WBS Prep


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinecarnage11
Mushroom Magician
Male User Gallery


Registered: 04/02/11
Posts: 3,409
Loc: 407 Flag
Last seen: 4 years, 10 months
Re: The whole syringe got sucked into one jar... [Re: FooMan]
    #15157838 - 09/30/11 02:24 PM (9 years, 7 months ago)

With PFTek you should have a dry layer of verm on the top. With the dry verm you don't need the tape.  PFTek jars should not be airtight. Make sure you have 4 inoculation holes in the lid.


--------------------
You're breathing so I guess you're still alive
Even if signs seem to tell me otherwise.



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineslothrop
Stranger
Registered: 09/30/11
Posts: 3
Last seen: 9 years, 4 months
Re: The whole syringe got sucked into one jar... [Re: carnage11]
    #15157985 - 09/30/11 02:54 PM (9 years, 7 months ago)

Really? I was going by the video, which used a layer of dry verm AND tape. The holes were in fact a little small - or rather the syringe needle is really damn thick, so that could definitely have been the problem.

So I've got all these extra jars - I should get a new syringe, widen the holes, unscrew the lids a tiny bit so that they're not airtight, sterilize them again, and try again?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblea2thej
Cosmic Cowboy
 User Gallery

Registered: 07/21/11
Posts: 687
Loc: Desert
Re: The whole syringe got sucked into one jar... [Re: slothrop]
    #15158011 - 09/30/11 03:00 PM (9 years, 7 months ago)

ya vaccumme that sucks man, if u get screwed lemme know ill send u a print

u should be fine though u have more to work with at all?
u can also re use the syringe as well with sterile water


--------------------
If i didnt leave u a trade rating plz remind me



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineHoneydew
Physicist
Male


Registered: 09/22/11
Posts: 241
Loc: The Yog Tower
Last seen: 8 years, 3 months
Re: The whole syringe got sucked into one jar... [Re: a2thej]
    #15158034 - 09/30/11 03:06 PM (9 years, 7 months ago)

in the videos they use micro-spore tape (medical tape) using other types of tape can cause a vacume because as it cooks the pressure in the jar builds and is released through your inoc points

when the jar cools it wants to suck the air back in to equalize the pressure, when it does this it will pull the tape up against the inoc hold and the adhesive from being cooked will cool and harden making a seal

that least this is what i think happened


--------------------
I'm only on this website to role play about mycology I would never actually do that.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinecarnage11
Mushroom Magician
Male User Gallery


Registered: 04/02/11
Posts: 3,409
Loc: 407 Flag
Last seen: 4 years, 10 months
Re: The whole syringe got sucked into one jar... [Re: slothrop]
    #15158331 - 09/30/11 04:10 PM (9 years, 7 months ago)

Quote:

slothrop said:
Really? I was going by the video, which used a layer of dry verm AND tape. The holes were in fact a little small - or rather the syringe needle is really damn thick, so that could definitely have been the problem.

So I've got all these extra jars - I should get a new syringe, widen the holes, unscrew the lids a tiny bit so that they're not airtight, sterilize them again, and try again?



I just use foil. The foil doesn't make it airtight, it just helps to keep water out of the jars while steam sterilizing. You remove the foil to inoculate the jars, and at that point you can leave the foil off. The dry layer of vermiculite creates the contaminate barrier from that point on.


--------------------
You're breathing so I guess you're still alive
Even if signs seem to tell me otherwise.



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineNullface

Registered: 09/04/11
Posts: 4,734
Loc: USA Flag
Last seen: 3 years, 1 month
Re: The whole syringe got sucked into one jar... [Re: carnage11]
    #15158413 - 09/30/11 04:32 PM (9 years, 7 months ago)

The opposite happened to me. I got some vermiculite stuck in the needle and when I pushed it out I accidentally squirted 4cc into one hole.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinecarnage11
Mushroom Magician
Male User Gallery


Registered: 04/02/11
Posts: 3,409
Loc: 407 Flag
Last seen: 4 years, 10 months
Re: The whole syringe got sucked into one jar... [Re: Nullface]
    #15158486 - 09/30/11 04:49 PM (9 years, 7 months ago)

Quote:

Nullface said:
The opposite happened to me. I got some vermiculite stuck in the needle and when I pushed it out I accidentally squirted 4cc into one hole.



If you get the needles with the beveled tip, and then point the needle toward the glass, it should help to prevent that problem.


--------------------
You're breathing so I guess you're still alive
Even if signs seem to tell me otherwise.



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineHoneydew
Physicist
Male


Registered: 09/22/11
Posts: 241
Loc: The Yog Tower
Last seen: 8 years, 3 months
Re: The whole syringe got sucked into one jar... [Re: carnage11]
    #15158648 - 09/30/11 05:19 PM (9 years, 7 months ago)

Quote:

carnage11 said:
Quote:

Nullface said:
The opposite happened to me. I got some vermiculite stuck in the needle and when I pushed it out I accidentally squirted 4cc into one hole.



If you get the needles with the beveled tip, and then point the needle toward the glass, it should help to prevent that problem.



you can always just heat the needle up so the verm shoots out the end, but beveled needles would be awesome im going to have to look into that


--------------------
I'm only on this website to role play about mycology I would never actually do that.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag   Amazon ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* A Few Questions from Beginner using Quart Jar Way happycamper 1,646 4 05/16/02 06:49 PM
by happycamper
* Re: Jars not sterilized yet but a fungus has started growing oOjonahOo 1,222 6 05/20/00 06:04 PM
by Anonymous
* jar lids during sterilization zodiac312 4,544 12 03/13/02 08:06 PM
by Anonymous
* I am scared to do a Print/syring!
( 1 2 all )
fearforce7 7,300 24 04/23/20 06:13 PM
by poisoned
* Re: quart jars?? Humidity 961 4 04/18/00 12:42 AM
by Humidity
* syringe question.. zazz 1,115 5 07/23/02 01:07 PM
by FanaTEK
* Accidentaly sucked a little brf into LC syringe. Will i be ok? flyhighfunguy 289 6 03/04/18 11:07 AM
by Neo1
* Question about 1/2 pint jars and innoculation Novacaine 761 3 04/04/02 12:41 AM
by WakingUpLate

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
1,223 topic views. 40 members, 231 guests and 68 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Everything Mushrooms
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.053 seconds spending 0.025 seconds on 13 queries.