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Offlineibuprofen88
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Registered: 09/30/11
Posts: 3
Last seen: 13 years, 3 months
contamination woes
    #15157130 - 09/30/11 09:15 AM (13 years, 3 months ago)

First and foremost: why can't you just sterilize mushrooms suspected of being contaminated? Boiling in water may be adequate; but since I have heard that contaminants may protect themselves with endospores, an autoclave/pressure cooker at 121°C would be sufficient- but not high enough to destroy pscilocybin. What is wrong with this idea?

This was my first attempt at growing; it was mainly exploratory so I tried several teks. I originally had several 2 litre coke bottles containing cakes as well as some of those modular storage tupperware containers that contained several crumbled cakes in casing. These didn't do well, so I moved them to a larger tupperware container. I started to get decent, but not great flushes.

I've collected probably 15 or 20 dry grams so far, but I'm worred about contamination. I didn't really think about it as I was harvesting, figuring that if they grew it would be safe.

One of the cakes growing in a 2 litre bottle had a good amount of greenish/blue splotching, and some of the shrooms were discolored, so I threw it out; but not after harvesting several flushes. Picture below is how it looked when I threw it out, but it was similarly discolored before:



The mushrooms that were growing in casing looked ok, but I have just noticed this blackish hue inside the body:



So I guess the general consensus would be to just throw out what I've harvested? It will hurt to throw out that many shrooms, especially because I've never actually had them and I was planning on doing it soon.

If so, is there any good reason not to just heat the shit out of them and consume, with the worst case scenario being they do nothing?

Edited by ibuprofen88 (09/30/11 09:18 AM)

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OfflineThe Influence
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Re: contamination woes [Re: ibuprofen88]
    #15157151 - 09/30/11 09:22 AM (13 years, 3 months ago)

I would wait for someone with more experience on this matter, but it looks like the ones in your pics of the cake might just be aborting. Maybe from the lack of nutrients because as you said you got several flushes off of it?

Though from my reading it is not that uncommon for cakes or casings to become contamed after a few flushes (casings more so than cakes) As I said I would wait for someone with more experience but I would not throw nothing out until then. Also how are you drying your flushes? The full grown stem turning black leads me to believe that the mushroom was beginning to rot before it got suffciently dried.

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Offlineibuprofen88
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Re: contamination woes [Re: The Influence]
    #15159458 - 09/30/11 06:42 PM (13 years, 3 months ago)

Actually that picture is of a mushroom that was fairly recently picked, it's still wet. I'm drying them on a mesh screen over silica gel in a small closed box, so I doubt that's the problem. Plus, the discoloration is always at the base.

I'm fairly certain I was way over-misting. I was using perlite, but I misted probably 20 sprays a day in addition adding water regularly.

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OfflineHoneydew
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Re: contamination woes [Re: ibuprofen88]
    #15159477 - 09/30/11 06:46 PM (13 years, 3 months ago)

sounds like over watering, what temp were they at?


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I'm only on this website to role play about mycology I would never actually do that.

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OfflineKumara
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Re: contamination woes [Re: Honeydew]
    #15159788 - 09/30/11 08:05 PM (13 years, 3 months ago)

On that second pic, are you sure that's not just bruising?

Greenish-blue splotches also sound like bruising - trich is bright green. 
I don't see anything wrong with that cake - mine start to get a little brown here and there after a few flushes due to drying out and aging, not contamination.
I still feel like a noob after nearly 3 years so I'll defer to more knowledgeable opinions.

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OfflineThe Influence
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Re: contamination woes [Re: Kumara]
    #15159863 - 09/30/11 08:31 PM (13 years, 3 months ago)

I don't know about over watering. I know it's possible, but I mist like crazy and two nights ago my pins were not growing nearly as fast as other people claim theirs grow; then I noticed that the cakes were dry and beginning to blue so I misted the shit out of them and squirted 5cc's of water under each cake and woke up this morning to the mushrooms almost tripled in size and even more pins coming out.

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OfflineWing
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Re: contamination woes [Re: The Influence]
    #15160044 - 09/30/11 09:14 PM (13 years, 3 months ago)

That's definitely very dark bruising from misting to much. I've seen it several times on my tubs when I leave some pins on there to hopefully grow and then I really wet down the sub to rehydrate.

Didi you spray the cake directly or just overly wet it?


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Offlineibuprofen88
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Re: contamination woes [Re: Wing]
    #15165281 - 10/02/11 03:16 AM (13 years, 3 months ago)

Thanks for the replies!

They are at probably around 78°F most of the time, the might get a few degrees higher if the AC is turned off. As I understand the upper limit for growing is somewhere around 80 degrees, and 85 for inoculation... does that sound right?

I had been basically spraying the cakes directly multiple times per day to the point of standing water. I stopped misting completely and am just venting more frequently, and my healthy cakes just started growing faster and larger than ever.

I guess I'm going to be having my first trip soon :mushroom2:

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OfflineXFINITY
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Re: contamination woes [Re: ibuprofen88]
    #15165701 - 10/02/11 07:57 AM (13 years, 3 months ago)

Quote:

ibuprofen88 said:
Thanks for the replies!

They are at probably around 78°F most of the time, the might get a few degrees higher if the AC is turned off. As I understand the upper limit for growing is somewhere around 80 degrees, and 85 for inoculation... does that sound right?

I had been basically spraying the cakes directly multiple times per day to the point of standing water. I stopped misting completely and am just venting more frequently, and my healthy cakes just started growing faster and larger than ever.

I guess I'm going to be having my first trip soon :mushroom2:




Incubation should be around room temperature 70F (20C) the cakes or spawn generate enough of their own heat to keep them usually around 5 degrees F higher than their surroundings. Now to talk about contamination, if you are unsure you should probably just throw them away. Better safe than sorry, especially if you consider that some bacteria and other pathogens can create toxins that could kill you. If that is the only small amount of mushrooms that look almost black it wouldn't be worth it to try and salvage.

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OfflineWing
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Re: contamination woes [Re: XFINITY]
    #15166973 - 10/02/11 01:11 PM (13 years, 3 months ago)

Cubes are a tropical species so they are able to grow in tmps between 60F-100F or even higher. But when it comes to conditions where contaminates are lower, then room temp to 80F is most optimal.


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OfflineThe Influence
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Re: contamination woes [Re: Wing]
    #15166993 - 10/02/11 01:16 PM (13 years, 3 months ago)

Quote:

winged_1 said:
Cubes are a tropical species so they are able to grow in tmps between 60F-100F or even higher. But when it comes to conditions where contaminates are lower, then room temp to 80F is most optimal.



I don't think they can handle much higher than 100 degrees, thermal death is at 106F I believe.

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