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OfflineFr0ggy
AmphibianExperimenter

Registered: 12/13/02
Posts: 85
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Last seen: 12 years, 8 months
Quick agar question
    #1514673 - 05/02/03 01:36 PM (14 years, 7 months ago)

Is it possible to prepare/sterilize agar without a pressure cooker?

Anyone had sucess with this?


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OfflineJeRiKo1
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Registered: 02/25/03
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Re: Quick agar question [Re: Fr0ggy]
    #1514730 - 05/02/03 02:09 PM (14 years, 7 months ago)

Sure, why not. I am not a mod, but I think this doesnt belong in Advanced Cult


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"All statements are true in some sense, false in some sense, meaningless in some sense, true and false in some sense, true and meaningless in some sense, false and meaningless in some sense, and true and false and meaningless in some sense.''


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Offlinecanid
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Re: Quick agar question [Re: JeRiKo1]
    #1514764 - 05/02/03 02:22 PM (14 years, 7 months ago)

i second that.

in answer to your question, sterilization can be achieved via microwave.


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If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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OfflineFr0ggy
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Registered: 12/13/02
Posts: 85
Loc: Amazonian Lily Pad
Last seen: 12 years, 8 months
Re: Quick agar question [Re: JeRiKo1]
    #1514839 - 05/02/03 02:47 PM (14 years, 7 months ago)

Someone feel free to to move this thread if they feel it needs to go in the mushroom cultivation forum, it's just that I was after reliable advice and not some bullshit newbie reply like yours JeRiKo1 ! You didn't even help in any way with my question so what was the point in posting apart from upping your number of posts ?

Thanks for the microwave tip concretefeet,  :smile: I couldn't find anything in the Shroomery FAQ about microwave sterilization so I'll try searching through the threads. 


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Edited by Fr0ggy (05/02/03 02:51 PM)


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Offlinecanid
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Re: Quick agar question [Re: Fr0ggy]
    #1514859 - 05/02/03 02:55 PM (14 years, 7 months ago)

you should find some with a search. a lot of people are using microwaves for sterilization lately, and with good results apearantly.

ignore JeRiKo1: though while you may be right about his response, he did answer your question properly considering the form in wich you worded it.

good luck to you.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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OfflineFr0ggy
AmphibianExperimenter

Registered: 12/13/02
Posts: 85
Loc: Amazonian Lily Pad
Last seen: 12 years, 8 months
Re: Quick agar question [Re: canid]
    #1514977 - 05/02/03 03:35 PM (14 years, 7 months ago)

Shit.. I just remembered my microwave died the other day and I'm waiting for a new motor or something for the repair guy to fit.. I keep on putting food in there and go to switch it on forgetting it's dead lolzz!  :grin:

I've had sucess with fractional sterilization/tyndallization with whole grains, can a similar method be used to sterilize agar? 


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OfflineJeRiKo1
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Re: Quick agar question [Re: Fr0ggy]
    #1515038 - 05/02/03 03:55 PM (14 years, 7 months ago)

What the fuck ? You asked if agar can be sterilized in a pressure cooker, and for sure it can. If you've wanted more advice you should've asked something like

"... or is there a better, easier, whatever way to do it ?"


--------------------
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Offlinecanid
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Re: Quick agar question [Re: JeRiKo1]
    #1515045 - 05/02/03 03:57 PM (14 years, 7 months ago)

[s/he] asked if it could be sterilized wothout a PC.

now can everyone just fuck and make up? it's just a misunderstanding.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


Edited by concretefeet (05/02/03 03:59 PM)


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OfflineJeRiKo1
Stranger

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Loc: Germany
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Re: Quick agar question [Re: canid]
    #1515051 - 05/02/03 03:58 PM (14 years, 7 months ago)

uuups.

/me takes everything back and walks towards the corner to stand there in shame.

[edit] Very sorry about that, if i bothered anyyone i wanna say a big FORGIVE ME to them.


--------------------
"All statements are true in some sense, false in some sense, meaningless in some sense, true and false in some sense, true and meaningless in some sense, false and meaningless in some sense, and true and false and meaningless in some sense.''


Edited by JeRiKo1 (05/02/03 04:01 PM)


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OfflineFr0ggy
AmphibianExperimenter

Registered: 12/13/02
Posts: 85
Loc: Amazonian Lily Pad
Last seen: 12 years, 8 months
Re: Quick agar question [Re: JeRiKo1]
    #1515142 - 05/02/03 04:16 PM (14 years, 7 months ago)

No problem and apologies for the short remark  :smile:  I'm just frustrated as to how to use the damn stuff without a PC... 


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Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 138
Re: Quick agar question [Re: Fr0ggy]
    #1516796 - 05/03/03 04:15 AM (14 years, 7 months ago)

In my opinion agar can not be sterilized without a PC unless you want to go with Tyndallization. A good way to test your sterilization is to make some agar and leave it uninoculated. If the agar is not sterile, most or all of the bacteria that escaped sterilization can be seen growing below the surface as tiny lense shaped colonies. Agar that has not been sterilized with a PC can be used successfuly for only as long as the bacteria remain trapped inside the agar. Eventually the bacteria will break through to the surface and, if they do, you are screwed.


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OfflineFr0ggy
AmphibianExperimenter

Registered: 12/13/02
Posts: 85
Loc: Amazonian Lily Pad
Last seen: 12 years, 8 months
Re: Quick agar question [Re: woodrow]
    #1517074 - 05/03/03 08:10 AM (14 years, 7 months ago)

OK, I managed to find some info on sterilizing without a PC !

Thanx for the input people  :smile: 


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