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OfflineFungal-one
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What to do with a petri dish bursting with life?
    #15145847 - 09/28/11 07:19 AM (13 years, 3 months ago)

I have two petri dishes that I knocked up with some GT spores back in July. The plates are fully colonized to say the least. Surprisingly there are no green or discolored spots, just an even brown across both plates. I'm aware of the need for sterilisation etc, no need to stress that here. My question is what to do with what's in my dishes to grow them to the next level. Liquid culture? Grain LC?

I'm just wondering how to proceed here. Any help would be greatly appreciated.


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OfflineCheeseBurgler
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Re: What to do with a petri dish bursting with life? [Re: Fungal-one]
    #15145983 - 09/28/11 08:19 AM (13 years, 3 months ago)

personally, i would use a section to noc up a grain jar (or 6), then make grain lc from that.

you could just drop a piece into some lc, but im weird about opening my lc jars after they come out of the PC.

you actually have a TON of options here, but i would never suggest something that i dont have experience with.


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Invisibledtowntoker
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Re: What to do with a petri dish bursting with life? [Re: CheeseBurgler]
    #15146002 - 09/28/11 08:23 AM (13 years, 3 months ago)

Why is it brown?  Have you isolated at all?  That should be your first step if the brown isn't contamination, then wedge->grain master ->glc or g2g up to you.


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OfflineFungal-one
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Re: What to do with a petri dish bursting with life? [Re: CheeseBurgler]
    #15146015 - 09/28/11 08:26 AM (13 years, 3 months ago)

Can you point me to refs on preparing a grain jar for impregnation? The method you had in mind.

Thanks for the input, greatly appreciated.

Why is it brown; Dunno, I figured that was just what color it is. I've never grown in a petri dish before.

The brown doesn't look like contamination. It is very clear brown. Not turbid at all.


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.

Edited by Fungal-one (09/28/11 08:29 AM)

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OfflineLord_McLovin
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Re: What to do with a petri dish bursting with life? [Re: Fungal-one]
    #15146116 - 09/28/11 08:48 AM (13 years, 3 months ago)

I, personally, would just do another transfer and look how it grows. Healthy cube myc is white, not brown.

EDIT: If the culture is this old and does not look healthy anymore (brown), you might just want to restart with a healthy young culture from spores.
Is it an isolate? If so, try to save it by doing another transfer. MS tissue just isn't worth the work.


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Edited by Lord_McLovin (09/28/11 08:54 AM)

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OfflineMOPE
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Re: What to do with a petri dish bursting with life? [Re: Lord_McLovin]
    #15146169 - 09/28/11 09:00 AM (13 years, 3 months ago)

I would go GLC route as well, as long as your agar isnt contaminated

here's the best tek I know of...

much easier than G2G imo.


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Offlinedr harry bush
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Re: What to do with a petri dish bursting with life? [Re: MOPE]
    #15146251 - 09/28/11 09:23 AM (13 years, 3 months ago)

i would go light malt and yeast lc ..... some of my dishes look abit brown on the underside if i have left the petris for a long time


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Offlinesteelmonkey
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Re: What to do with a petri dish bursting with life? [Re: dr harry bush]
    #15146575 - 09/28/11 11:01 AM (13 years, 3 months ago)

It shouldn't be brown...period..if it is it's contaminated

I'd love to see a pic of this "brown" mycelium


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Offlinedr harry bush
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Re: What to do with a petri dish bursting with life? [Re: steelmonkey]
    #15146812 - 09/28/11 12:09 PM (13 years, 3 months ago)

Quote:

steelmonkey said:
It shouldn't be brown...period..if it is it's contaminated

I'd love to see a pic of this "brown" mycelium




top of agar - alt ms (no contams)



bottom of dishes 1x alt7 ms and the other is lions mane - all my cubes are in storage in a culture fridge ... my lab is busy being cleaned so i dont want to go pull them out, when i do ill take pictures.



i think this is the brown he may be talking about.


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OfflineXerdge
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Re: What to do with a petri dish bursting with life? [Re: steelmonkey]
    #15146940 - 09/28/11 12:40 PM (13 years, 3 months ago)

Quote:

steelmonkey said:
It shouldn't be brown...period..if it is it's contaminated

I'd love to see a pic of this "brown" mycelium




Brown doesn't seem right to me either.

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InvisiblePinsWellWithOthers
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Re: What to do with a petri dish bursting with life? [Re: Xerdge]
    #15147508 - 09/28/11 02:52 PM (13 years, 3 months ago)

Brown always seems to end up being a bacterial contamination to me but as said. Pictures halp a lot.

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OfflineFungal-one
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Re: What to do with a petri dish bursting with life? [Re: PinsWellWithOthers]
    #15148561 - 09/28/11 05:47 PM (13 years, 3 months ago)

Yep, this is it.


I don't have the dishes with me or I'd take a pic. I actually gave them to someone who has never grown and I'm asking on their behalf. Next time I grow it'll be a few syringes of penis envy nice and fresh from my neighborhood spore shop.


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.

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InvisiblePinsWellWithOthers
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Re: What to do with a petri dish bursting with life? [Re: Fungal-one]
    #15149347 - 09/28/11 08:08 PM (13 years, 3 months ago)

I wouldn't touch those dishes with a thirty-nine-and-a-half foot pole if that is how they look from the tops!

Edited by PinsWellWithOthers (09/28/11 08:10 PM)

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Offlinesteelmonkey
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Re: What to do with a petri dish bursting with life? [Re: PinsWellWithOthers]
    #15149397 - 09/28/11 08:15 PM (13 years, 3 months ago)

At the very least just for experiments sake take a grain sized piece from a leading edge and transfer it to a fresh agar plate and see what happens

I definately wouldn't be doing any transfers to grain with brown mycelium though


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Offlinedr harry bush
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Re: What to do with a petri dish bursting with life? [Re: steelmonkey]
    #15150871 - 09/29/11 01:14 AM (13 years, 3 months ago)

Ok this is my opinion on what this brown is...... because in true fact it is not brown but i slightly darker colour of the MEA due to two reasons.

1st where the mycelium is thickest it is a darker colour, so less light can pass though causing a slightly darker spot.

2nd metabolism – common name – mycelium piss ...... have you ever noticed this on your cakes, trays or tubs ? same this happens on a petri. I normally notice this darker area before i see a pin form on a plate.

Please do correct me if the above is incorrect


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OfflineFungal-one
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Re: What to do with a petri dish bursting with life? [Re: dr harry bush]
    #15153917 - 09/29/11 04:26 PM (13 years, 3 months ago)

If I had the plates I would take lots of pics for you guys, but I don't have them here.

I think I'll just order syringes. Thanks alot for the input.


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.

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OfflineLord_McLovin
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Re: What to do with a petri dish bursting with life? [Re: Fungal-one]
    #15153938 - 09/29/11 04:29 PM (13 years, 3 months ago)

Don't you have any spores left? You don't need many.


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OfflineFungal-one
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Re: What to do with a petri dish bursting with life? [Re: Lord_McLovin]
    #15154288 - 09/29/11 05:46 PM (13 years, 3 months ago)

I got bunches, but they arent sterile. They are just wrapped up in foil and have been all over the country with me, even while I was in the hospital for over a month.


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.

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OfflineLord_McLovin
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Re: What to do with a petri dish bursting with life? [Re: Fungal-one]
    #15154610 - 09/29/11 06:51 PM (13 years, 3 months ago)

If your spores were sterile I'd feel bad for you. :smirk:
You could continue your agar experience and try to clean the culture obtained from the spores. :shrug:
But I guess getting another print/syringe would be better in case you're lazy.


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OfflineFungal-one
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Re: What to do with a petri dish bursting with life? [Re: Lord_McLovin]
    #15155231 - 09/29/11 08:57 PM (13 years, 3 months ago)

I'm not lazy, so much as I am busy. At the moment I just don't have the attention to spare something as delicate and precise and that would need to be. I'll be moving in a few weeks (again) and maybe then I'll feel up to something like that then, but now, no fucking way.


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.

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