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OfflineLucidFenix119
Lucid Mind
Registered: 04/27/03
Posts: 27
Last seen: 20 years, 4 months
MycoBag Question
    #1511460 - 05/01/03 01:21 PM (20 years, 11 months ago)

When is the appropiate time to stir up the substrate in a mycobag to ensure that all of it is colonized. It seems everytime someone mentions the need to do this, they never seem to include when to do it.

-Fenix

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Invisiblez@z.com
Libertarian
Registered: 10/13/02
Posts: 2,876
Loc: ATL
Re: MycoBag Question [Re: LucidFenix119]
    #1511564 - 05/01/03 02:02 PM (20 years, 11 months ago)

Do it when the mycelia starts colonizing (probably around 15% or so). Just spread it evenly around the bag so that it will colonize faster.


--------------------
"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis

"I would rather be exposed to the inconveniencies attending too much liberty than to those attending too small a degree of it." - Thomas Jefferson

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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: MycoBag Question [Re: z@z.com]
    #1512050 - 05/01/03 04:58 PM (20 years, 11 months ago)

that sounds about right  :smile:

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OfflineFunkyBudah
myco-wizard

Registered: 04/25/03
Posts: 430
Loc: Shakedown Street
Last seen: 17 days, 3 hours
Re: MycoBag Question [Re: MycoFactory]
    #1512827 - 05/01/03 08:13 PM (20 years, 11 months ago)

My uncle's felching hampster did it at about 20%, and after a day or two, they just took off. A couple didn't get going too fast, so after a couple days of slow growth, he shook again. Presto!

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InvisibleTerrapinSunrise
Stranger
Registered: 01/27/03
Posts: 350
Loc: KY
Re: MycoBag Question [Re: FunkyBudah]
    #1513284 - 05/01/03 10:21 PM (20 years, 11 months ago)

hey guys, my friend has a mycobag that, after mixing around 30% colonization, has stalled all growth for 2 weeks. she doesnt have any sterile water or sterile needles - regardless, she doesn't think the substrate is too dry. anybody have an idea why her bag stalled? it is possible that it was due to temperature fluctuations. in that case, will the mycelium just give up, or will it eventually make an attempt to continue colonizing? thanks for the help - ill pass it on to her. peace

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Anonymous

Re: MycoBag Question [Re: TerrapinSunrise]
    #1513368 - 05/01/03 10:52 PM (20 years, 11 months ago)

Bacteria is most likely the caus, my friend has encournterd this in the past. Most of the bacterial contamination created a nasty rust color fluid in the bottom of the bag. Hope this helps.

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Offlinecanid
irregular meat sprocket
Male User Gallery

Folding@home Statistics
Registered: 02/26/02
Posts: 11,912
Loc: looking for zeebras, n. c...
Last seen: 3 months, 6 days
Re: MycoBag Question [Re: fee]
    #1513863 - 05/02/03 02:15 AM (20 years, 11 months ago)

you can mix bags several times over the course of colonization. te only real problem is that if there is a small localized contamination that would otherwise be overcome, it can be spread throughout the uncolonized substrate more easily.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.

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OfflineLucidFenix119
Lucid Mind
Registered: 04/27/03
Posts: 27
Last seen: 20 years, 4 months
Re: MycoBag Question [Re: canid]
    #1513951 - 05/02/03 03:45 AM (20 years, 11 months ago)

Thanks for the help guys :smile:.

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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: MycoBag Question [Re: LucidFenix119]
    #1515091 - 05/02/03 02:05 PM (20 years, 11 months ago)

It is very likely that if mycelium doesn't recover after shaking it is from bacteria or another contaminant. Sometimes contamination goes un-noticed until the culture is shaken up. Many cultivators use this as a way to determine whether they have a clean culture or not before using it to inoculate larger amounts of materials.

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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: MycoBag Question [Re: MycoFactory]
    #1517721 - 05/03/03 02:26 PM (20 years, 11 months ago)

for a bag of seeds....your well informed.

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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: MycoBag Question [Re: Prisoner#1]
    #1517844 - 05/03/03 03:45 PM (20 years, 11 months ago)

:smile:

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InvisibleTerrapinSunrise
Stranger
Registered: 01/27/03
Posts: 350
Loc: KY
Re: MycoBag Question [Re: MycoFactory]
    #1522102 - 05/05/03 02:26 PM (20 years, 11 months ago)

well the mycelium has continued its growth. however, someone noticed that the bottom of the mycobag's substrate is a little dry perhaps due to the reptile heater. is the only way to remoisturize done by injecting sterile water? if so, how can you make sterile water? can one boil distilled water and h202 and then let the syringe suck it up? would that work? thanks

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Offlineministry
Networkn' Guru

Registered: 03/21/03
Posts: 30
Loc: Chattavegas
Last seen: 20 years, 11 months
Re: MycoBag Question [Re: TerrapinSunrise]
    #1522288 - 05/05/03 03:36 PM (20 years, 11 months ago)

What kind of substrate is it?

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InvisibleTerrapinSunrise
Stranger
Registered: 01/27/03
Posts: 350
Loc: KY
Re: MycoBag Question [Re: ministry]
    #1522328 - 05/05/03 03:52 PM (20 years, 11 months ago)

mycobag substrate is a combination of vermiculite and rye berries i *think*.

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