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OfflineDcyfr
No one inparticular.

Registered: 04/29/03
Posts: 20
Last seen: 3 years, 1 month
Making your own substrate bags...
    #1510599 - 05/01/03 07:28 AM (20 years, 10 months ago)

The other day I stumbled across a post stating how to make your own substrate bags using polypropylene bags. I was wondering if someone could post the link or give their methods as how to accomplish this. I'm tired of using jars all the time. One other question though. If you make the bags and inoc. strait through the plastic, how do you allow for proper gas exchange without contam?

Thanx.

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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Making your own substrate bags... [Re: Dcyfr]
    #1510615 - 05/01/03 07:40 AM (20 years, 10 months ago)

Best bet is to buy high quality autoclave bags. Makeshift bags are a pita & often fail - for any number of reasons. 6T  :tongue:


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Making your own substrate bags... [Re: Dcyfr]
    #1510698 - 05/01/03 08:29 AM (20 years, 10 months ago)

Here is a way to make Rye grass seed in bags. Just substitute another substrate and use the same basic out line.
Rye grass seed bag tek
In the coming weeks I will be adding more teks like this for other substrates.

Don't have a HEPA hood? we now carry a autoclavable bag with injector port and micro filter that can be sealed before it is autoclaved. It should be available on our site by later today.

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Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 6 months
Re: Making your own substrate bags... [Re: Dcyfr]
    #1511046 - 05/01/03 10:31 AM (20 years, 10 months ago)

I make my own - it's easy. Get polyproplyene bags and attach square Tyvek filter patches with a termoresistant glue.

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OfflinePuffPuffGive
journeyman

Registered: 01/29/03
Posts: 54
Last seen: 20 years, 1 month
Re: Making your own substrate bags... [Re: zeronio]
    #1511173 - 05/01/03 11:25 AM (20 years, 10 months ago)

What I have done is after I fill up my jars with popcorn I take the rest of the popcorn and dump it into a ziplock, cut a whole into the bag and put a tyveck strip on it just by taping it on their. I just do this so i dont waste anything but I havent had any contams yet. But I'm also not ready to get rid of jars becuase of this.

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Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 6 months
Re: Making your own substrate bags... [Re: PuffPuffGive]
    #1511195 - 05/01/03 11:36 AM (20 years, 10 months ago)

If you use polypropylene bags than you can sterilize them.

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