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InvisibleMunchauzen
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Urban Contaminated BRF Disposal Tek (deleted)
    #15109661 - 09/20/11 10:26 PM (13 years, 3 months ago)

.

Edited by Munchauzen (11/12/11 11:07 PM)

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Offlineasdfasdf


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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15110071 - 09/21/11 12:25 AM (13 years, 3 months ago)

How about PCing (or steaming if you have no PC), dumping, then cleaning?  Why heat it the second time?


--------------------
"FlexXx said:
To answer some of your questions...If by "ms" you mean "magic shrooms" then yes that's what I'm trying to grow"

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Offlinemorbiddoctor
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Re: Contaminated Spawn Disposal Tek [Re: asdfasdf]
    #15110418 - 09/21/11 03:17 AM (13 years, 3 months ago)

I've always dumped my sub or flung it out my window with a sharp arm jerk, cap them then submerged in bleach water until whenever, usually leave them about a day or so. Then wash with really hot water and dish soap.

I should probably be wearing a mask but I can hold my breath a While. Not That that prevents any spores from landing on my person. I've had trich issues off and on but never had an issue reusing a jar after this.


--------------------
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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: asdfasdf]
    #15110421 - 09/21/11 03:20 AM (13 years, 3 months ago)

Quote:

asdfasdf said:
How about PCing (or steaming if you have no PC), dumping, then cleaning?  Why heat it the second time?




there is no second time? it only gets heat once, when I boiled them at the end.
sterilize with bleach->dump->boil

its just my logic that the safest and most powerful tool for sterility should be the very last step, not the first.

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OfflineJaffyJaffar
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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15110550 - 09/21/11 05:33 AM (13 years, 3 months ago)

i like but for extra measure (if i get contams) will probably boil the shit out of the jar 1st then do your bleach water tek - extra measure

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Offlineasdfasdf


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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15112236 - 09/21/11 02:21 PM (13 years, 3 months ago)

Quote:

Munchauzen said:
Quote:

asdfasdf said:
How about PCing (or steaming if you have no PC), dumping, then cleaning?  Why heat it the second time?




there is no second time? it only gets heat once, when I boiled them at the end.
sterilize with bleach->dump->boil

its just my logic that the safest and most powerful tool for sterility should be the very last step, not the first.




Oh whoops I was thinking of your old method when I said that.

Really?  I completely disagree.  You want it as sterile as possible BEFORE you open it, not after.  Your going to sterilize them again anyway the next time you use them.

Why kill some stuff, open the jar, then make sure everything is dead?


--------------------
"FlexXx said:
To answer some of your questions...If by "ms" you mean "magic shrooms" then yes that's what I'm trying to grow"

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InvisibleMr. Anderson
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mRe: Contaminated Spawn Disposal Tek [Re: asdfasdf]
    #15112255 - 09/21/11 02:25 PM (13 years, 3 months ago)

I don't make contaminated jars lol


--------------------


Disclaimer: All posts are completely fictional and or for educational purposes only.

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Offlinedontkillthedj
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Re: Contaminated BRF Disposal Tek [Re: Munchauzen]
    #15112316 - 09/21/11 02:39 PM (13 years, 3 months ago)

lol it seems like every tome i see a problem in my grow, i see a fresh post for it on shroomery.

i like it, gonna go get rid of my trich jar now.


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Offlinek00laid
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Re: Contaminated BRF Disposal Tek [Re: dontkillthedj]
    #15112390 - 09/21/11 02:53 PM (13 years, 3 months ago)

i just pressure cook contamd jars.

and open them outdoors


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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: asdfasdf]
    #15112485 - 09/21/11 03:11 PM (13 years, 3 months ago)

Quote:

You want it as sterile as possible BEFORE you open it, not after.  Your going to sterilize them again anyway the next time you use them.




1 part bleach per 100 parts water will release plenty of peroxide and sodium hypochlorite onto its foes, thoroughly sterilizing (it makes them pop like balloons, clump up together, and die) - I recommend dish gloves. I like to boil to clean residuals from the bleach 100%, and anything else left in there. Might be overkill, but hey I'm slightly OCD.

As for the jars, they were all nocced with a purchased albino a+ syringe. All four jars contam'd on every single injection point. I believe I was given a contaminated syringe. Not a single A+ albino did not contam, and the other B+ and PE uncut jars I have are colonizing very nicely right now, and not a single one of those contam'd!

Also, I live in a big apartment complex, surrounded by other apartment complexes. I can't dispose of anything outside. Would be way too shady...

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Invisiblemaug
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Re: Contaminated BRF Disposal Tek [Re: k00laid]
    #15112513 - 09/21/11 03:16 PM (13 years, 3 months ago)

I do something similar to the op. I soak in soapy water, in a bucket. Then take outside, dump in the compost, wash out the jars with soap and hot water, and take a shower. Works well enough for me. I don't like to PC or use cleaning chemicals when I don't need to.

I don't do agar work, and I think that's why I think that's why RR was such a perfectionist in the vids. You're bad habits tend to show up in the agar.


--------------------
I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes

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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: JaffyJaffar]
    #15112569 - 09/21/11 03:28 PM (13 years, 3 months ago)

Quote:

JaffyJaffar said:
i like but for extra measure (if i get contams) will probably boil the shit out of the jar 1st then do your bleach water tek - extra measure




Thanks, like I said, I'm no scientist. I'm sure you could combine these steps in any random order and still get decent results. Here's some more info that might help...

Health department's recommendation for a sterile dish tank is 100 parts per million.
Health department's limit on dish tanks is 200 parts per million.
Beer brewers use 1 part per 100, then rinse off with distilled water, to avoid imparting bleach flavor to their beer, as it can leave clingy residuals.

For advanced sterilization, this is a great read from a book named "Brewing Techniques"
http://www.brewingtechniques.com/library/backissues/issue1.2/raines.html
kind of an overview of advanced sterilization as a jumpoff for more research.

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Offlinesinewave
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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15112909 - 09/21/11 04:47 PM (13 years, 3 months ago)

Injection with bleach seems a good idea, then let the contents soak inside the jar with the bleach overnight.

That should kill damn near anything before ever opening the thing to the outside.

:laser:


--------------------
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Invisiblemaug
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Re: Contaminated Spawn Disposal Tek [Re: sinewave]
    #15113100 - 09/21/11 05:38 PM (13 years, 3 months ago)

Chlorine bleach is horrible for the environment, and I don't use it in any stage of my growing. Chlorine bleach also has all sorts of nasty by-products like chloroform and trihalomethanes that you should really stay away from, and that you shouldn't dump in your compost/drain. These byproducts will now be coating your jars.

Chlorine bleah would not even work as well as safer products like chlorine dioxide (pool/aquarium cleaner, water purification drops). ClO2 will activate in 20 minutes, while chlorine bleach needs a few hours. "because household bleach is diluted to only 5% bleach, you would need 17 drops of bleach for one drop of the mixed ingredient in Aquamira[ClO2 product. I have maximum strength pool cleaner, which is an undiluted ClO2 source]" (source). ClO2 also does not have many of the harmful by-products of chlorine bleach. I still wouldn't use ClO2 in any stage of my growing either, other than maybe air-bombing.

If you soak the jars/bags in water, there shouldn't be any/many air-born spores. Even if they are, you shouldn't be emptying them or soaking them in your work area to begin with! The water will be a massive spore slurry. You can then empty your jars/bags into the bucket, and hose them off outside. Wash them out with soapy water, and wipe them down with ethanol or isopropyl if you so choose. This will not leave any residue. As always, take a shower and change your clothing before going back to your grow area. Imho PCing before you pack your jars/bags with substrate is over-kill, but it's a hell of a lot better than using bleach.


--------------------
I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes

Edited by maug (09/21/11 06:02 PM)

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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15113425 - 09/21/11 06:49 PM (13 years, 3 months ago)

Quote:

maug said:
Chlorine bleach is horrible for the environment




wives tale. current practices make it completely safe to use bleach. its highly reactive and highly unstable which means it doesn't last long when mixed into an aqueous environment. the production of bleach is even waste-water free (with the exception of cleaning the equipment) http://esis.jrc.ec.europa.eu/doc/existing-chemicals/risk_assessment/REPORT/sodiumhypochloritereport045.pdf

Quote:

Chlorine bleach also has all sorts of nasty by-products like chloroform that you should really stay away from, and that you shouldn't dump in your compost/drain. These byproducts will now be coating your jars.



Might be misleading. Sodium hypochlorite can bond with acetone, butane, surfactants, fragrances, or isopropyl alchohol to form chloroform. Its not inherently in bleach, and does not coat anything if chloroform never forms. Also, the sodium hypochlorite must be at a concentration of at least 40% in the solution to be considered hazardous to oxidize into a harmful byproduct. From there to 1% is moderate, and less than that its very safe(NFPA 430, 2000)

Quote:

The water will be a massive spore slurry.



Sodium hypochlorite is used to sanitize drinking water, so I doubt it would be a veritable "spore slurry"

So sodium hypochlorite combined with certain organic household items can form harmful compounds. Not surprising, but then why does this exist?? Virocidin-X Thats what they use in hospitals. Its sole active ingredient is sodium hypochlorite, and has both surfactants and fragrances?! Chemistry is not my strong suit, but I believe its because the %'s in the mixture, like I mentioned earlier, that make it safe to use. That shit even kills AIDS :eek:

thanks for the response Maug, it got me to do some learnin'... :cheers:

Edited by Munchauzen (09/21/11 07:02 PM)

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Invisiblemaug
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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15113713 - 09/21/11 07:43 PM (13 years, 3 months ago)

Yes it's highly reactive. Not to be condescending or anything, but that's how it works as a sanitizer. It oxidizes and reacts with things, forming new compounds in the process. If it's used properly then within a day the bulk of the active chemical will no longer be in it's original state. This is because it is in the process of forming thousands of byproducts, depending on what it's conditions/environment are. Chloroform is one, trihalomethane is another. I don't think it's possible to list/know them all. If it's watered down, then I'd imagine the only difference is that you'll have to use more of the impure solution.

Hospitals use bleach because they are either working with massive amounts of surface area that need to be sanitized, or because autoclaving is not feasible. Safe use for humans isn't necessarily safe for your mushroom spores. >>>Because I look at bleach's use as an unknowable wild card, I do not use it.<<<

Quote:

Munchauzen said:
Quote:

The water will be a massive spore slurry.



Sodium hypochlorite is used to sanitize drinking water, so I doubt it would be a veritable "spore slurry"



My point was that the spores will be in the water, not air. This alone should greatly reduce the risk of the spores getting into your work area. If you're making 20 jars of grain a month... imo you don't need to take extra precautions like bleach or an additional round of PCing. You won't see a difference.

This is what I would recommend. Just to clairify, I don't use/recommend bleach.
Quote:

maug said:
I still wouldn't use ClO2 in any stage of my growing either, other than maybe air-bombing.

If you soak the jars/bags in water, there shouldn't be any/many air-born spores. Even if they are, you shouldn't be emptying them or soaking them in your work area to begin with! The water will be a massive spore slurry. You can then empty your jars/bags into the bucket, and hose them off outside. Wash them out with soapy water, and wipe them down with ethanol or isopropyl if you so choose. This will not leave any residue. As always, take a shower and change your clothing before going back to your grow area. Imho PCing before you pack your jars/bags with substrate is over-kill, but it's a hell of a lot better than using bleach.




--------------------
I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes

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OfflineJaffyJaffar
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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15113721 - 09/21/11 07:44 PM (13 years, 3 months ago)

Throw the jar away

End discussion/








Lol

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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: JaffyJaffar]
    #15114082 - 09/21/11 08:54 PM (13 years, 3 months ago)

The most typical thing to oxidize out of it at normal household uses would be some calcium scaling, from prolonged exposure to low amounts of calcium hypochlorite contained in the bleaching powder found in liquid bleach. Some bleaches have anti-scalers though, so that might not even happen.

Thanks again to all for the input. I might write some more sanitation teks at a later date, some of the brewers teks look neat. fractional sterilization sounds like it might be really good for making a giant 1 gallon mason jar LC that doesn't fit into anything I have.

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OfflineBig Pappa
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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15114120 - 09/21/11 09:03 PM (13 years, 3 months ago)

geez sounds like a LOT of work!!!  I just toss it in the trash, and go buy a whole flat (12 jars) for less than 10 bucks!  quicker, cleaner, no mess no fuss.

I can see doing all that if you like live away from any sort of civilization... 

And if its too 'shady" throwing them out in your trash because you live in apartments.. then just put it in a bag and drive buy your local gas station or "sonic drive through and toss it in there trash!


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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: Big Pappa]
    #15114153 - 09/21/11 09:10 PM (13 years, 3 months ago)

Quote:

Big Pappa said:
geez sounds like a LOT of work!!!  I just toss it in the trash, and go buy a whole flat (12 jars) for less than 10 bucks!  quicker, cleaner, no mess no fuss.

I can see doing all that if you like live away from any sort of civilization... 

And if its too 'shady" throwing them out in your trash because you live in apartments.. then just put it in a bag and drive buy your local gas station or "sonic drive through and toss it in there trash!




see OP, at the very end.
that $.88 jar costs can cost over $100 in environmental degradation which in turn leads to lower valued ecosystem services, and that's not even counting the lid. You're basically chunking liters upon liters of petrol when you trash a set of jars.

Edited by Munchauzen (09/21/11 09:10 PM)

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OfflineBig Pappa
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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15114209 - 09/21/11 09:20 PM (13 years, 3 months ago)

wow... my eyes glazed over when I got down to "contents" on that wiki page...  no offence intended, but where I live all the trash pickup is recycled...  it all goes to the plant in my hometown where it is separated and then reused. 

See, in MY mind, I may have thrown out an 88 cent jar, but in fact I helped my economy by giving something of value (however small it may be) that in turn creates jobs, and revenue for others.

There is no "Land Fill" around here...  its ALL either recycled or incinerated.


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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: Big Pappa]
    #15114317 - 09/21/11 09:39 PM (13 years, 3 months ago)

There is no land fill in my county either. All we have is recycling. However, that does not stop the municipal board from sending trash to the closest county that does have one.

They aren't going to recycle a mason jar. Recycling facilities *ONLY* recycle glass that comes from food or drink products. Windows, cups, jars, tumblers, all that stuff is not going to get recycled.

Reason being, all they do is clean it, crush it, and sell it back to food packagers. The pepsi bottler will only want glass from old glass bottles.

if you like the idea of helping the economy, check out kiva.org I've made several 0% interest loans to struggling people who collect glass and paper and return them to manufacturing plants for recycling. never had any of them default on me either.

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Offlinedontkillthedj
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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15115291 - 09/22/11 01:28 AM (13 years, 3 months ago)

i think anyone who throws away glass jars might as well be using sterilized substrate bags instead, they're alot less of a burden on the environment when you toss them.

if you're tossing glass jars, you're missing the point. glass jars will last a long time, and you can absolutely sterilize them again. throwing them out is just lazy.



...unless the jar has worms, then throw that shit out.


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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15115367 - 09/22/11 01:49 AM (13 years, 3 months ago)

I agree, just toss the cake, not the jar (..esp if you're paranoid, think PRINTS+5-0 :wink:)  I had a 1/2 pint BRF cake contam pretty bad.  Sorry, no pics; I kept it around for a while just to see how the contam would affect the myc.. not a bad freak show; green/yellow/brown. 

Ended up making a trash bag hazmat t-shirt, took the jar to the dumpster, opened and emptied inside a plastic grocery bag -> file 13'd the cake and trash bag outside.  Wash->sterilize the empty jar.

I feel that was a decent procedure to reduce my exposure to the contams, but I suppose you could then (once inside, blinds drawn :blush:) strip naked for a full scrub down if you feel contam'd yourself.


--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic.  Click and READ the first link.  I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/

Edited by igigi (09/22/11 02:12 AM)

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Offlinedontkillthedj
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Re: Contaminated Spawn Disposal Tek [Re: igigi]
    #15115429 - 09/22/11 02:15 AM (13 years, 3 months ago)

Quote:

igigi said:
I agree, just toss the cake, not the jar..  I had a 1/2 pint BRF cake contam pretty bad.  Sorry, no pics; I kept it around for a while just to see how the contam would affect the myc.. not a bad freak show; green/yellow/brown. 

Ended up making a trash bag hazmat t-shirt, took the jar to the dumpster, opened and emptied inside a plastic grocery bag -> file 13'd the cake and trash bag outside.  Wash->sterilize the empty jar.

I feel that was a decent procedure to reduce my exposure to the contams, but I suppose you could then (once inside, blinds drawn :blush:) strip naked for a full scrub down if you feel contam'd yourself.




just got done sterilizing 8 plastic 1/4 pint jars, 2 glass 1/4 pt jars and one 1/2 pint jar that were all stalled out or contam'd.

i need to redesign my 1/4 pint plastics, not one of them showed any growth after 3 weeks... wet spot. smelled sour.

oh well, got a tub of clean stuff ready to refill!


--------------------
You mess with the shark young man, you get the shark!

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Invisiblemaug
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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15115491 - 09/22/11 02:41 AM (13 years, 3 months ago)

Quote:

dontkillthedj said:
just got done sterilizing 8 plastic 1/4 pint jars, 2 glass 1/4 pt jars and one 1/2 pint jar that were all stalled out or contam'd.

i need to redesign my 1/4 pint plastics, not one of them showed any growth after 3 weeks... wet spot. smelled sour.

oh well, got a tub of clean stuff ready to refill!




Work on your moisture content. Should be 1 cup brf, 1 cup water, 2 cups verm. And don't flood it when you inoculate - it already has perfect moisture content. If there's too much water, it's guaranteed to contam with bacteria.

Glass jars can get worms? You mean maggots? Cause there aren't a lot of worms in my grains or brf. Just drown them and/or toss them in the compost.


--------------------
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InvisibleMunchauzen
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Re: Contaminated Spawn Disposal Tek [Re: igigi]
    #15115503 - 09/22/11 02:45 AM (13 years, 3 months ago)

Quote:

igigi said:
Ended up making a trash bag hazmat t-shirt




hahaha how about that for a mental image. sounds like charlie work.

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15115552 - 09/22/11 03:03 AM (13 years, 3 months ago)

Long story short, right? lol  But, I think you've got something, especially w/ the opening the jar under water to prevent spores from flying about, all nibbly bimbly and such.



@maug - bleach is fine all around.


--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic.  Click and READ the first link.  I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/

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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15115578 - 09/22/11 03:16 AM (13 years, 3 months ago)

Quote:

maug said:
Quote:

dontkillthedj said:
just got done sterilizing 8 plastic 1/4 pint jars, 2 glass 1/4 pt jars and one 1/2 pint jar that were all stalled out or contam'd.

i need to redesign my 1/4 pint plastics, not one of them showed any growth after 3 weeks... wet spot. smelled sour.

oh well, got a tub of clean stuff ready to refill!




Work on your moisture content. Should be 1 cup brf, 1 cup water, 2 cups verm. And don't flood it when you inoculate - it already has perfect moisture content. If there's too much water, it's guaranteed to contam with bacteria.

Glass jars can get worms? You mean maggots? Cause there aren't a lot of worms in my grains or brf. Just drown them and/or toss them in the compost.




yeah they were some experiment jars with my first finished LC, turned out that i injected too much and i've just been waiting this whole week to dispose of them and noc up some freshies with the oak ridge LC i have. i'm sure now that this next round will go smoothly. learning alot.

and i havent got any maggots in my jars but i've seen pics f them and it fucking freaks me out. i'd flip my shit if i saw a maggot in my jars.

seriously, i'd probably fucking lose it. haha :jimcarreysplit:


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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15145550 - 09/28/11 04:35 AM (13 years, 3 months ago)

So just an update. I caught strep from soaking jars. I made 15 jars more than I could inoculate, and they eventually contaminated with bacteria. I scratched off the tape and submerged them in a bucket filled with water, but when I pulled them out some of them were not even wet inside. I took them outside and dumped them in the compost. I thoroughly hosed off the jars, put them back in the bucket, and filled it up with water. Brought them inside and stirred in a few drops of ClO2. I could tell it worked in about 10-15 minutes by how the glass went from slippery to "squeaky clean". But it was only a minute or so after I stopped stirring that I could feel the sore throat starting.

Don't soak. Even if the jars are mostly colonized, you're creating the perfect environment for bacteria. And brf is not very selective.

You could have a fogger/mister spray over the jars. Submerge them and slowly open them underwater. Have a fan blowing away from you. But in retrospect, soaking for any moderately long period of time seems like a dumb idea. Adding a chemical to kill spores already trapped in the water is pointless, as it's the airborne ones that are a concern. If anything you risk creating a super-strain that is resistant to your sanitation methods.


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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15145566 - 09/28/11 04:46 AM (13 years, 3 months ago)

did you put in the ClO2 on the initial soak before you ever opened them? if not, that's what my tek was recommending, to kill the baddies in the jar first.  I'm sorry to hear this either way...

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15145602 - 09/28/11 05:09 AM (13 years, 3 months ago)

The strep is under control, so it's ok. And no, I added it after I dumped the brf.

I doubt that the chlorine would penetrate the brf very well. Water has a hard enough time getting in. So you'll still have to open the jars releasing air bubbles containing spores. Basically you're in the same situation as just slowly opening up the jars slowly under water, and dumping the brf out; you're counting on the water to trap the spores. I added the chlorine after emptying out the jars because that's when everything was exposed, and when it would be most effective for cleaning the jars for future grows.

Imo, a good fan outside > chlorine. Again, I think it's the airborne spores that you need to worry about. After dumping jars I take a shower and put on fresh clothing, I would think would take care of any spore-water that gets on me.

You're welcome to test any of it out. I did once, and I see no need to personally try it again.


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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15145623 - 09/28/11 05:28 AM (13 years, 3 months ago)

you've got me scared lol I'm gonna start wearing face masks filled with polyfill

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15145664 - 09/28/11 05:46 AM (13 years, 3 months ago)

Nah, I think an fan aimed outside the house is the best bet. It works for not breathing in lye vapor and other chemicals, it should work for this too.

For a funny story, Google search "paul stamets the blob". This is basically the same thing. On top of that, these polycultures could favor very resistant strains, because they have to fend off all the other bac/yeast and their set of antibiotics.


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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15145880 - 09/28/11 07:36 AM (13 years, 3 months ago)

Quote:

maug said:

So just an update. I caught strep from soaking jars...







Making this type of claim is how silly myths arise. 

First, to even know if you had a streptococcal bacteria infection which was causing your sore throat, you would have had to have gotten a throat culture done and analyzed by a lab or doctor.

Secondly, you would have also had to convince the lab to culture the material in your jars to determine if the same strain of bacteria was present in amounts which would have led to the infection....

My guess is that you did neither of those things.

And a super-strain of jar bacteria resistant to bleach.  Come on, stop scaring everyone...


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Edited by volare (09/28/11 07:37 AM)

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Re: Contaminated Spawn Disposal Tek [Re: volare]
    #15146908 - 09/28/11 12:32 PM (13 years, 3 months ago)

Quote:

volare said:
Quote:

maug said:

So just an update. I caught strep from soaking jars...







Making this type of claim is how silly myths arise. 

First, to even know if you had a streptococcal bacteria infection which was causing your sore throat, you would have had to have gotten a throat culture done and analyzed by a lab or doctor.

Secondly, you would have also had to convince the lab to culture the material in your jars to determine if the same strain of bacteria was present in amounts which would have led to the infection....

My guess is that you did neither of those things.

And a super-strain of jar bacteria resistant to bleach.  Come on, stop scaring everyone...



agreed


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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15147529 - 09/28/11 02:56 PM (13 years, 3 months ago)

Sorry if that seemed a little too dramatic, it just seems like there's so many better methods. You could also inject water into the jar with a syringe. I'm kinda grumpy from being sick too.

To me it seems like I could correlate being sick to the bacteria in the bucket because I could feel the sore throat starting minutes after I started cleaning the jars. It's been three days since then, and I'm still sick. My tonsils have light swelling, have white puss on them, and there's red spots on the roof of my mouth. I don't have money to throw away on a doctor, just to give it a name. Whether or not it's strep, imho the same risks still apply. Chlorine during the soak probably would have helped avoid getting sick, but a I still don't think sanitizing a substrate will have the same success rate as sanitizing water.

My only point really is that soaking brf creates a good environment for bacteria, and it should theoretically be about as effective at trapping mold spores as just opening the jars in the bucket. Anyways, I'm out of this thread.


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Re: Contaminated Spawn Disposal Tek [Re: maug]
    #15148639 - 09/28/11 06:02 PM (13 years, 3 months ago)

Quote:

maug said:
I could feel the sore throat starting minutes after I started cleaning the jars.




CORRELATION IS NOT CAUSATION

:kingcrankey:

:science:


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Re: Contaminated Spawn Disposal Tek [Re: k00laid]
    #15148756 - 09/28/11 06:24 PM (13 years, 3 months ago)

yeah i dont know why you didnt use bleach in your soak water, it kills everything... and maybe even lysol the air for good measure if you're worried about the bubbles from the jars letting out viable bacteria... i'm not, but if you really think that made you sick, then maybe you should take more preventative measures.


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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15150200 - 09/28/11 10:42 PM (13 years, 3 months ago)

or.... just open your jars outside?


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Re: Contaminated Spawn Disposal Tek [Re: k00laid]
    #15150255 - 09/28/11 10:57 PM (13 years, 3 months ago)

Or just pressure cook them.  Done.  Why is this thread still going?


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Re: Contaminated Spawn Disposal Tek [Re: asdfasdf]
    #15150276 - 09/28/11 11:01 PM (13 years, 3 months ago)

should pressure cook them before opening and open them outside


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Re: Contaminated Spawn Disposal Tek [Re: k00laid]
    #15150295 - 09/28/11 11:05 PM (13 years, 3 months ago)

the whole point of this thread originally was for people in urban environments who can't open outside and who are using the RR videos, and don't have pressure cookers.

However, I have devised a new idea without any chemicals to safely do this inside. I'll post it sooner or later...

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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15165108 - 10/02/11 01:31 AM (13 years, 3 months ago)

on 9/23 i used this this tek to sterilize 8 plastic 1/4 pt c jars and 4 glass 1/4 pt jars along with two 1/2 pt tall jars that all had trich/wet spot. to follow this, i mixed up some brf/verm and steam sterilized (didn't PC because i wanted to test the viability of this tek to actually sterilize.)

of all of the jars, every single one is colonizing nicely with no contams yet. two of the glass ones are about 1/2 colonized already.

i don't want to call it a success yet, but the jars i had in these jars before showed signs of contam earlier/didnt even start colonizing.


i reccomend it for city dwellers for sure.


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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15168818 - 10/02/11 08:01 PM (13 years, 3 months ago)

keep me posted!
as for mine...
9.30 I knocked up 13 jars, including the 4 jars that I had cleaned when originally doing this tek. Out of the whole lot, only 1 contaminated, and it was a brand new jar.
All the others are growing healthy myc!

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15179539 - 10/05/11 12:36 AM (13 years, 3 months ago)

figured i would toss you a pic because my first jars of these set hit 100% yesterday. FAST LITTLE FUCKERS!



the one i'm holding is 100%, the rest in the middle are 75-80% and those jars in the back were just birthed and waiting for me to wash again... didn't let these cakes consolidate too long, maybe 2 days, because i might crumble and case these as an experiment.

the plastic ones are smaller but are taking longer  to consolidate. strange. probably a GE thing.


...either way, i think i can say this is proof positive that this tek works for sterilizing jars both plastic and glass.


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Edited by dontkillthedj (10/05/11 12:50 AM)

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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15179593 - 10/05/11 01:04 AM (13 years, 3 months ago)

I took a gamble and did my plastic jars without a dry verm layer so I could break them up at 20% to speed things up. I steamed them with micropore tape over the inoc holes, shot through them, then applied some 91% iso moistened micropore tape on top of that to cover the hole.

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15179603 - 10/05/11 01:11 AM (13 years, 3 months ago)

i did the exact same thing the last set of plastic jars i did and they all contam'd.

i dont think these ziploc tubs have a very airtight seal around the edge.


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Re: Contaminated Spawn Disposal Tek [Re: dontkillthedj]
    #15179706 - 10/05/11 02:06 AM (13 years, 3 months ago)

good looks my new ones are getting taped up around the edges with duct tape when they come out of the steamer in about.... right now.

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15179717 - 10/05/11 02:14 AM (13 years, 3 months ago)

my method:
1.walk out back door
2.dump jar contents in compost
3.pour some bleach water into jar

never had a problem with it and no need to waste gas :smile:


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Re: Contaminated Spawn Disposal Tek [Re: Sexycybe]
    #15179739 - 10/05/11 02:23 AM (13 years, 3 months ago)

this
Quote:

Sexycybe said:
my method:
1.walk out back door
2.dump jar contents in compost
3.pour some bleach water into jar

never had a problem with it and no need to waste gas :smile:




my tek was specifically for those who can't do this outside. e.g. urban settings, apt complexes, etc. i edited the OP to say urban tek.

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Re: Contaminated Spawn Disposal Tek [Re: Munchauzen]
    #15179762 - 10/05/11 02:39 AM (13 years, 3 months ago)

Quote:

Munchauzen said:
this
Quote:

Sexycybe said:
my method:
1.walk out back door
2.dump jar contents in compost
3.pour some bleach water into jar

never had a problem with it and no need to waste gas :smile:




my tek was specifically for those who can't do this outside. e.g. urban settings, apt complexes, etc. i edited the OP to say urban tek.



Ahk, i wasn't having a go at ya (i think you know that tho) :smile:
That is good for those peeps :cool:


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Re: Contaminated Spawn Disposal Tek [Re: Sexycybe]
    #15179884 - 10/05/11 04:23 AM (13 years, 3 months ago)

Fuck this tek, I'm busting out the owlbox :wink:

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