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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15098617 - 09/18/11 10:21 PM (13 years, 3 months ago)

Take the foil off? I guess I should get some medical breathing tape to put over then inoculation points then?


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OfflineThe Influence
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15098670 - 09/18/11 10:29 PM (13 years, 3 months ago)

Did you use a dry verm layer? If so this would be enough to protect them. I used micropore tape as added protection, but have read of plenty of success without it. If you did use the dry verm layer, take the foil off and do not put that fan over the top of them, that would most likely just force contams down into the jar. They really just need to sit out in the open.

If you didn't use a dry verm layer I don't know what to do then?

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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15098711 - 09/18/11 10:40 PM (13 years, 3 months ago)

Fortunately, I did put a dry verm layer on the top. I'm just going to go ahead and ditch the chamber and keep them up in the kitchen cabinets for a few days without the foil and see what happens.


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OfflineThe Influence
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15098826 - 09/18/11 11:06 PM (13 years, 3 months ago)

Quote:

shamanamba said:
Fortunately, I did put a dry verm layer on the top. I'm just going to go ahead and ditch the chamber and keep them up in the kitchen cabinets for a few days without the foil and see what happens.



:thumbup:

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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15099175 - 09/19/11 01:18 AM (13 years, 3 months ago)

So here's what I got going on now.

Since I have 12 jars to play with, I've decided to make this situation into an experiment.

Taking into consideration the things I've learned so far, I've again made some more tweaks to the incubation chamber rather than ditching it altogether.

I am keeping 3 jars with the micro-pore tape over the inoculation points in the chamber, and 3 jars without the tape.

I am doing the same thing with the other 6 jars in the kitchen cabinet.

Pics below.







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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15102293 - 09/19/11 05:42 PM (13 years, 3 months ago)

just checked on the jars today and found something growing! Worms!

... worms...

Yep. One of my jars has worms.


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The Shaman

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OfflineMushroom Wisperer
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15102330 - 09/19/11 05:49 PM (13 years, 3 months ago)

The fuck?

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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Mushroom Wisperer]
    #15102345 - 09/19/11 05:53 PM (13 years, 3 months ago)

I know, especially after having been PC'd for two hours prior to inoculation... those must have been some hard freakin core worm eggs!


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OfflineWing
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15102369 - 09/19/11 05:58 PM (13 years, 3 months ago)

They had to have gotten in there after you PC'd.


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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Wing]
    #15102448 - 09/19/11 06:10 PM (13 years, 3 months ago)

That does stand to reason, but I just have a hard time imagining any bug getting into the incubation chamber, under the foil, through the inoculation hole, under the dry verm layer and then laying eggs... The worms are way down in the jar towards the bottom.


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OfflineWing
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15102462 - 09/19/11 06:13 PM (13 years, 3 months ago)

pics?


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Offlineshamanamba
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Wing]
    #15102562 - 09/19/11 06:28 PM (13 years, 3 months ago)

I just tried to snap a pic but its nearly impossible because of the texture on surface of the jars and how small the worms are. Even though I see them with my eyes, the worms just seem to blend in with the substrate in the pictures.


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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15102571 - 09/19/11 06:30 PM (13 years, 3 months ago)

:frown: Keep your eye on the other jars. I hope it all turns out well for you.


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OfflineThe Influence
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Wing]
    #15103043 - 09/19/11 07:57 PM (13 years, 3 months ago)

Are they moving? You might just have the start to some ropey mycelium.

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Invisiblebuffer
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15103883 - 09/19/11 10:44 PM (13 years, 3 months ago)

I can't beleive you've got worms after pressure cooking! Take pics man.. this is an odd one for sure :eek:

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Offlineurbanguru
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15104849 - 09/20/11 04:55 AM (13 years, 3 months ago)

Quote:

shamanamba said:
just checked on the jars today and found something growing! Worms!

... worms...

Yep. One of my jars has worms.




I just wanted to say that i to thought that i had worms in a few jars(10) that i had to dump out due to bad spore syringe. The jars didnt contaminate but i had zero colonization after a month. anyway when i opened them up to dump them out and i noticed what i thought looked like little worms about the size and shape of a medium sized maggot. I touched them with the fork and they just decintigrated. i never saw any movement or anything like that and I still have no idea what they were. It had nothing to do bad sterilization because i did the 10 jars in two sets of five along with two other sets of five from a different syringe. both time were weeks apart and the total of 10 jars from the other syringe all colonized normally and fruited as such. no contams watsoever. like i said i have no idea what they where but the only place they could have come from was the syringe.

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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: urbanguru]
    #15104857 - 09/20/11 05:05 AM (13 years, 3 months ago)

Oh these little guys are squirming around. There's no doubt about it, they're worms alright.



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Offlinerainx
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15104880 - 09/20/11 05:21 AM (13 years, 3 months ago)

Absolem



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Offlineurbanguru
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15104895 - 09/20/11 05:35 AM (13 years, 3 months ago)

Quote:

shamanamba said:
Oh these little guys are squirming around. There's no doubt about it, they're worms alright.






Those look identical to what I had in mine but I never saw them move. Maybe they were dead after being in the jars for a month. It's a mystery to me.
Still no colonization?

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Invisiblebuffer
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Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: urbanguru]
    #15104900 - 09/20/11 05:41 AM (13 years, 3 months ago)

That is fucking disgusting and makes me wanna barf. Sorry.

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