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InvisibleFungus_monk
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Will i have to restart?
    #15092969 - 09/17/11 08:45 PM (9 years, 7 months ago)

To start off, if you are going to tell me to buy a pc than please do not reply because i am content with steam sterilization.

So i was able to successfully steam sterilize some popcorn over the course of three days and i just picked up a couple more jars today and some rtv high temp silicone. I've got a sterile jar of popcorn waiting to be shot up but i only used polyfill in the lid because it was brought to my attention that you can just inoculate through polyfill but im not really comfortable doing that.

Since i got the silicone today i wanted to go ahead and make a self healing port on another lid so i dont have to worry about shooting through the polyfill.
Now my question is, if i go ahead and do this and transfer the popcorn to the new jar will i have to restart my 3 day steaming session?
I know i will have to resterilize it but idk for how long.


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InvisibleHesy-Ra
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Re: Will i have to restart? [Re: Fungus_monk]
    #15093025 - 09/17/11 08:59 PM (9 years, 7 months ago)

I might try to just inoculate using an alcohol soaked sponge on the needle (from a tek somewhere here) and throw a band-aid on it. Not sure what condition the corn may end up in going through the steam process again.


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InvisibleFungus_monk
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Re: Will i have to restart? [Re: Hesy-Ra]
    #15093039 - 09/17/11 09:01 PM (9 years, 7 months ago)

Quote:

Hesy-Ra said:
I might try to just inoculate using an alcohol soaked sponge on the needle (from a tek somewhere here) and throw a band-aid on it. Not sure what condition the corn may end up in going through the steam process again.



No offense but i dont really see how that applies to me. Im not worried about inoculation.


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InvisibleHesy-Ra
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Re: Will i have to restart? [Re: Fungus_monk]
    #15093064 - 09/17/11 09:05 PM (9 years, 7 months ago)

Quote:

Fungus_monk said:
I've got a sterile jar of popcorn waiting to be shot up but i only used polyfill in the lid because it was brought to my attention that you can just inoculate through polyfill but im not really comfortable doing that.




Am i missing something here?


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InvisibleFungus_monk
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Re: Will i have to restart? [Re: Hesy-Ra]
    #15093081 - 09/17/11 09:10 PM (9 years, 7 months ago)

My apologies but i dont see what you are trying to get at.
I dont want to inoculate through the polyfill, i will also not be using an alcohol soaked sponge because alcohol sanitizes, not sterilizes.
My question was if i put the popcorn into another jar will i have to start the sterilization process all over or not. I dont think i would because all of the endospores in the grain have already been killed from the previous sterilization, i plan on re sterilizing anyway because i know i will have to for some period of time regardless. I just dont want to have to do it for three days ago unless it is necessary.


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OfflineSexySmurff
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Re: Will i have to restart? [Re: Fungus_monk]
    #15093146 - 09/17/11 09:27 PM (9 years, 7 months ago)

you could sterilize your new lid and put it on the jar.  i do that all the time with my lcs so i can shake them.


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Edited by SexySmurff (09/17/11 09:37 PM)


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InvisibleFungus_monk
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Re: Will i have to restart? [Re: SexySmurff]
    #15093193 - 09/17/11 09:36 PM (9 years, 7 months ago)

Cool thanks man


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OfflineWing
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Re: Will i have to restart? [Re: SexySmurff]
    #15093204 - 09/17/11 09:38 PM (9 years, 7 months ago)

Quote:

SexySmurff said:
you could sterilize your new lid and put it on the jar.  i do that all the time with my lcs so i can shake them.




:whathesaid: Thats seems the best option if you don't want to resterilize your grains. Do it in a glovebox


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Invisibleprismism
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Re: Will i have to restart? [Re: Fungus_monk]
    #15094318 - 09/18/11 03:21 AM (9 years, 7 months ago)

I know this isn't what you want to hear, but what makes you so sure that your popcorn is sterile in the first place? I love using popcorn as spawn, but I would think it would be the hardest grain to sterilize without a pressure cooker.


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OfflineSexySmurff
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Re: Will i have to restart? [Re: prismism]
    #15094325 - 09/18/11 03:25 AM (9 years, 7 months ago)

i had a question about 3 days sterilization too.  methinks if he's using popcorn he must be steaming for quite awhile.


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Offlinesinewave
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Re: Will i have to restart? [Re: Fungus_monk]
    #15094559 - 09/18/11 06:06 AM (9 years, 7 months ago)

Id say that any time your sub is exposed to the open air for any reason (before its 100% colonized that is), your probably going to need to  re-sterilize.


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OfflineSexySmurff
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Re: Will i have to restart? [Re: sinewave]
    #15094564 - 09/18/11 06:09 AM (9 years, 7 months ago)

im sure he's gonna be doing it inside a glovebox. 

if i take your advice g2g would be impossible.


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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InvisibleFungus_monk
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Re: Will i have to restart? [Re: SexySmurff]
    #15094820 - 09/18/11 09:52 AM (9 years, 7 months ago)

I sterilized for a total of twelve hours over the course of three days. And i had injected another jar that was done with this leftover jar with some lc and it is looking good.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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