Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineshamanamba
The Shaman
Male User Gallery

Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
Trusted Cultivator
14 days, One jar has... something?... (BRF cakes) [Now with pics]
    #15085600 - 09/16/11 12:41 AM (13 years, 4 months ago)

This year has been my 3d or 4th attempt at growing p. cubensis, following the PF Tek method. So far i'm on my second batch of 12 half pint jars.

The first batch went much better, although only 3 of the 12 jars reached full colonisation without contamination. But those were prepared in a crappily sterilized bathroom.

Before starting on this second batch, I made a glove box and an incubation chamber so this time, the preparation was much more steryl and conditions have been controlled more closely.

I have been waiting on this second batch to show signs of mycelium growth for five days now and every little bit of the substrate is still devoid of any white at all. I understand that it can take longer than this to happen but it just seems odd.

The following factors seem like they may be the cause but I just wanted to run this by everyone on here.

1.  :syringe:  The spore vendor sent me 8 syringes. I used 2, maybe 3 on the first 12 jars, then the rest of the syringes sat in the fridge for a couple months until 5 days ago when I used another 2 syringes to inoculate this second batch of 12 jars. I noticed that small white fuzzies were floating around in the syringes. Donno whats up with that but I went ahead and inoculated the jars anyway.



2.  :sun:  I had the incubation chamber set to 86 degress for the first 3 days, but later, I read on here that its better to keep them incubating at 75-80 degrees. The chamber has been sitting at a steady 77 degress for the past 2 days.



I am thinking of "re-innoculating" the jars if nothing shows up in the next few days, but i'm wondering if the added water from the syringes will mess things up even more... (?)

I will post back with more relevant details when I think of them

Any advice and information is greatly appreciated and thankyou in advance to anyone who takes the time to shed some light my way


--------------------
The Shaman

Edited by shamanamba (09/25/11 04:20 AM)

Extras: Filter Print Post Top
OfflineThe Influence
Free Sheeks
Male User Gallery


Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 1 day
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15085640 - 09/16/11 01:07 AM (13 years, 4 months ago)

You need to go to youtube and search "let's grow mushrooms PF tek parts 1-4". Watch all of them, take notes then watch again. I did this and am on my first grow now, have 8 of 10 jars at 100% colonization at 2 weeks the other two jars will be there in a day. No contams on any of the jars. I didn't do anything special except do everything exactly as the videos showed, no cutting corners.

If you used two to three syringes on twelve jars, that threw off your water content right there. You only need a 1/4 ml per inoc hole, thats 1 ml/cc per jar. You should have at least gotten 10 jars out of one syringe.

Also do not keep your jars in an incubation chamber, this prevents gas exhange, place them out in the open on a shelf. The dy verm and micropore tape will protect them.

Extras: Filter Print Post Top
Offlineshamanamba
The Shaman
Male User Gallery

Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
Trusted Cultivator
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15085698 - 09/16/11 01:47 AM (13 years, 4 months ago)

First off, thank you so much for the quick reply. I have seen those videos you mentioned but because I have seen so many different versions of the same tek, all claiming to be the most effective, I have just chosen bits and pieces from each source I have found and incorporated them into my procedure. I'll review those videos again and follow them to the "T" for my next batch.

In the mean time, i'm still open to any other input that anyone may have on the information I have provided above.


--------------------
The Shaman

Extras: Filter Print Post Top
OfflineEl Douche
El Douchius Maximus

Folding@home Statistics
Registered: 01/08/11
Posts: 538
Loc: Drippin Outa Da Vag Flag
Last seen: 13 years, 3 months
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15085713 - 09/16/11 01:59 AM (13 years, 4 months ago)

It is popular consensus that it can take up to 3 weeks to show growth. 5 days to 2 weeks being the norm.

I would not worry about over-hydration.

Room temp(70+) is fine for incubation.

Spore syringes go bad. Some last for 6 months, some only one month.


--------------------
I'm The Douche, Doucher, His Royal Doucheness, or El Doucherino if you are not into that whole brevity thing.

Trade List : Wanted/have edible cultures, ethobotanicals, cool plants, cacti.

Extras: Filter Print Post Top
OfflineThe Influence
Free Sheeks
Male User Gallery


Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 1 day
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: El Douche]
    #15085782 - 09/16/11 02:28 AM (13 years, 4 months ago)

Quote:

El Douche said:
It is popular consensus that it can take up to 3 weeks to show growth. 5 days to 2 weeks being the norm.

I would not worry about over-hydration.

Room temp(70+) is fine for incubation.

Spore syringes go bad. Some last for 6 months, some only one month.



I agree that 5-10 days to show growth would not be to far fetched, but if your not showing growth within 3 weeks, most likely something is wrong.

Extras: Filter Print Post Top
InvisibleStropharis
 User Gallery


Registered: 04/02/07
Posts: 1,854
Trusted Cultivator
OG Cultivator
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15085832 - 09/16/11 02:46 AM (13 years, 4 months ago)

86°?  Why?

Be patient, five days is nothing.

Don't mix and match your TEKs yet, stick to one.

Extras: Filter Print Post Top
OfflineDevolver
Honey Badger
Male User Gallery


Registered: 08/24/11
Posts: 114
Loc: Washington Flag
Last seen: 13 years, 1 month
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: Stropharis]
    #15085864 - 09/16/11 03:09 AM (13 years, 4 months ago)

Patience is the key. My first batch of jars took 10 days before they showed. Nothing you can do but wait right now.

Extras: Filter Print Post Top
OfflineJaffyJaffar
Nom Nom Nom
Male


Registered: 07/26/11
Posts: 1,700
Loc: AMERICA, FUCK YEAH!
Last seen: 12 years, 8 months
Re: Not even a hint to colonisation 5 days after inoculation (BRF cakes) [Re: Devolver]
    #15085890 - 09/16/11 03:27 AM (13 years, 4 months ago)

I read that growing myc produces up to 5 degrees internal temp....you gotta factor that into your final temp....if your at 77 then internally its 82

Extras: Filter Print Post Top
Offlineurbanguru
Male User Gallery

Registered: 09/11/11
Posts: 764
Loc: the swamp
Last seen: 4 months, 17 hours
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: JaffyJaffar]
    #15085974 - 09/16/11 04:50 AM (13 years, 4 months ago)

I read in RR notes that anything over 83 degrees drastically reduced colonization speed. I for one believe the man knows what hes talking about. Keep your jars around 75ish and you will be great. 1 cc per jar is a rule of thumb. Be patient and give them time to do their thing. up to 3 weeks IME.:scaryshroom:

Extras: Filter Print Post Top
OfflineThe Influence
Free Sheeks
Male User Gallery


Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 1 day
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: urbanguru]
    #15085986 - 09/16/11 04:57 AM (13 years, 4 months ago)

Quote:

urbanguru said:
I read in RR notes that anything over 83 degrees drastically reduced colonization speed. I for one believe the man knows what hes talking about. Keep your jars around 75ish and you will be great. 1 cc per jar is a rule of thumb. Be patient and give them time to do their thing. up to 3 weeks IME.:scaryshroom:



I dont believe he said it reduced colonization speed, just that there wasn't any faster colonization, and that temps upwards of 80 degrees is more optimal for contams to grow.

Jars do colonize pretty fast at the 72-75 degree range from my experience. Haven't tried any higher because it is not recommended as you pointed out, and 8 out of 10 of my jars are at 100% at the 2 week mark in the above mentioned temps.

Extras: Filter Print Post Top
OfflineDevolver
Honey Badger
Male User Gallery


Registered: 08/24/11
Posts: 114
Loc: Washington Flag
Last seen: 13 years, 1 month
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: The Influence]
    #15086012 - 09/16/11 05:16 AM (13 years, 4 months ago)

The quote I saw from RR stated roughly that "At 86 degrees colonization is only 2/3's as fast as 80 degrees".

Extras: Filter Print Post Top
OfflineJaffyJaffar
Nom Nom Nom
Male


Registered: 07/26/11
Posts: 1,700
Loc: AMERICA, FUCK YEAH!
Last seen: 12 years, 8 months
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Devolver]
    #15086022 - 09/16/11 05:30 AM (13 years, 4 months ago)

here's the rr quote

*I have found little to no difference in colonization speeds between 75 and 81F. Growth falls off rapidly at 83F and above, not 87F. That chart above is bogus, period. I have tried dozens of times to duplicate it and it can't be done. It was apparently made by someone who did ONE grow with sloppy note taking, and sent the results to Paul. Growth is much slower in cold temperatures until you hit 69F, where it speeds up quite a bit until about 75F, where it remains 'flat' until 81, then is flat again until 83, where it falls off fast beginning at 84. By 'flat' I mean there is no discernible increase or decrease in rate of growth within those ranges. Jars will colonize as fast at 75F as they will at 80F. I've proved this time and time again with every strain in my collection. Growth also falls off rapidly above 84, and this is why so many new folks have problems with incubators set at 86F, and jars that 'won't colonize'. The figures I give are substrate temperatures, not air temperatures. The temp inside the jar is 1 to 5 degrees higher than the surrounding air, depending on where in the colonization cycle the jar is. The heat produced falls off fast as the jar approaches full colonization. If you live in an igloo, (or near the waterfront) by all means build an incubator, but keep it in the normal room temperature ranges for best results. I see no reason to set one above 80F, and lots of reasons not to. Here's a picture of one of my shelves for colonizing jars. The substrate bags are there because I ran out of room on the other shelf. These are in a room at normal room temperature, and exposed to light nearly all day. I don't even put the pf jars on a top shelf where it's warmer. Of course, I had a good teacher on how to make them up, as everyone will soon know. COLONIZATION

Extras: Filter Print Post Top
Offlineurbanguru
Male User Gallery

Registered: 09/11/11
Posts: 764
Loc: the swamp
Last seen: 4 months, 17 hours
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Devolver]
    #15086077 - 09/16/11 06:11 AM (13 years, 4 months ago)

Quote:

Devolver said:
The quote I saw from RR stated roughly that "At 86 degrees colonization is only 2/3's as fast as 80 degrees".




I knew that's what he said I just didn't have the time to find the quote myself. I was guilty of overheating my jars at first too. Lots of misinformation floating around but I trust what RR has too say. :grin:

Extras: Filter Print Post Top
InvisibleStropharis
 User Gallery


Registered: 04/02/07
Posts: 1,854
Trusted Cultivator
OG Cultivator
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: urbanguru]
    #15086227 - 09/16/11 07:42 AM (13 years, 4 months ago)

:laugh2:

"RR said this"
"RR said that"
"no he didn't RR said this"
"RR this RR that"

Extras: Filter Print Post Top
Offlineshamanamba
The Shaman
Male User Gallery

Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
Trusted Cultivator
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Stropharis]
    #15094238 - 09/18/11 12:31 AM (13 years, 4 months ago)

ok its now at 7 days and still no signs of growth, but here's a new detail I was unaware of until tonight... my room mate just told me he pressure cooked the jars on maximum heat for 2 hours...


--------------------
The Shaman

Edited by shamanamba (09/18/11 12:32 AM)

Extras: Filter Print Post Top
OfflineBig Pappa
Challenged
Male User Gallery


Registered: 09/03/11
Posts: 717
Loc: Smurf Village Flag
Last seen: 11 years, 17 days
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15094267 - 09/18/11 12:46 AM (13 years, 4 months ago)

thats fine as long as the PC didnt run out of water... NEVER let it run dry!!!


--------------------


For Sale
Want List
 
We are not human beings having a spiritual experience. We are spiritual beings having a human experience.

Extras: Filter Print Post Top
OfflineWing
The Eye Tyrant
Male User Gallery


Registered: 04/25/11
Posts: 3,293
Last seen: 1 year, 4 months
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15094275 - 09/18/11 12:51 AM (13 years, 4 months ago)

Quote:

shamanamba said:
:syringe:  The spore vendor sent me 8 syringes. I used 2, maybe 3 on the first 12 jars, then the rest of the syringes sat in the fridge for a couple months until 5 days ago when I used another 2 syringes to inoculate this second batch of 12 jars. I noticed that small white fuzzies were floating around in the syringes. Donno whats up with that but I went ahead and inoculated the jars anyway.




2-3 syringes for 12 is a lot of spore solution. Without pics its hard to tell but could be too wet.


--------------------
My Old Grow Logs


Extras: Filter Print Post Top
Offlineshamanamba
The Shaman
Male User Gallery

Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
Trusted Cultivator
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Wing]
    #15094280 - 09/18/11 12:55 AM (13 years, 4 months ago)

I'll label the jars and take some pics soon


--------------------
The Shaman

Extras: Filter Print Post Top
OfflineDevolver
Honey Badger
Male User Gallery


Registered: 08/24/11
Posts: 114
Loc: Washington Flag
Last seen: 13 years, 1 month
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: shamanamba]
    #15094307 - 09/18/11 01:16 AM (13 years, 4 months ago)

2-3 syringes wouldn't make it too wet. If anything it would speed up the colonization slightly.

Extras: Filter Print Post Top
OfflineThe Influence
Free Sheeks
Male User Gallery


Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 1 day
Re: Not even a hint to colonization 5 days after inoculation (BRF cakes) [Re: Devolver]
    #15094324 - 09/18/11 01:24 AM (13 years, 4 months ago)

Quote:

Devolver said:
2-3 syringes wouldn't make it too wet. If anything it would speed up the colonization slightly.



Where did you here that from? There would be more spores competeing to germinate which would slow germinization, and also that much more water would defenitely saturate the substrate too much. This is where the saying more is not always better comes in.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* Will BRF Cakes Cont. to Colonize HAY?? KaliedoscopEyes 1,116 5 06/24/02 04:40 PM
by KaliedoscopEyes
* Casing BRF cakes (pics)
( 1 2 all )
primal_future 23,756 24 09/08/22 06:56 AM
by Screwup
* Spawning from BRF cakes. Juggles 8,353 7 01/24/02 10:03 AM
by Anonymous
* BRF cakes colonizing horse shnizzit (pics) primal_future 6,150 12 08/20/02 07:04 PM
by northernsoul2
* BRF cakes on perlite, good or bad. nutsymptom421 10,119 13 10/24/17 08:13 PM
by bodhisatta
* Can I use brf cakes for wheat straw cmb911 2,973 4 02/24/02 02:15 AM
by Zebedee
* SPAWNING POOP?! With BRF cakes indicaz 3,459 5 08/19/18 04:18 AM
by mushpunx
* Can I grind up BRF cakes & mix w/ Compost skoal 4,765 3 06/25/20 05:11 PM
by Cegda

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,205 topic views. 31 members, 157 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 14 queries.