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Amazon Shop: ½ Pint Jars, Agar

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Offlinexwave3000
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Registered: 02/27/03
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Are these too dark (scroll down for pic)
    #1507192 - 04/30/03 11:55 AM (14 years, 1 month ago)

? Cause I've heard having too many spores can be problematic.


Edited by xwave3000 (04/30/03 01:40 PM)


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Offlinefugu
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Re: Are these too dark [Re: xwave3000]
    #1507293 - 04/30/03 12:41 PM (14 years, 1 month ago)

dude what is your asking?
>too many spores can be problematic
where ?on casings,in jars,on mushes.on the side of your terrariums ,where?


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Offlinefugu
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Re: Are these too dark [Re: fugu]
    #1507298 - 04/30/03 12:43 PM (14 years, 1 month ago)

if the asking is about the syringes noi
there is no prob.
it s also better
but more spor solution is not good ........ not more spore
also all these talkings are for the fucking cakes


--------------------
mushroom culture history making ...Mr. Allan is the best .....


Edited by fugu (04/30/03 12:44 PM)


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Offlinexwave3000
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Re: Are these too dark [Re: fugu]
    #1507423 - 04/30/03 01:29 PM (14 years, 1 month ago)

oops sorry. Forgot to post the pic.


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Invisiblefailbot999
Registered: 02/18/02
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Re: Are these too dark *DELETED* [Re: xwave3000]
    #1507465 - 04/30/03 01:52 PM (14 years, 1 month ago)

Post deleted by ski_stoned

Reason for deletion: delete



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Offlinejakeholman
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Registered: 02/24/03
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Re: Are these too dark [Re: failbot999]
    #1507521 - 04/30/03 02:14 PM (14 years, 1 month ago)

darker is better, but you could make ten syringes out of one of those dark babies, and germination is still within a couple days. syringes that are completely clear with seemingly nothin in them will still work fine.
JakeH


--------------------
The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.


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OfflineAnnoA
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Re: Are these too dark [Re: xwave3000]
    #1507549 - 04/30/03 02:25 PM (14 years, 1 month ago)

Absolute waste of spores.


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OfflineEkstaza
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Re: Are these too dark [Re: Anno]
    #1507654 - 04/30/03 02:52 PM (14 years, 1 month ago)

Anno, if you do your own spore printing and make your own syringes, would it hurt to make syringes this dark.(i.e. contams more probable???)

I understand that it is a waste of spores, but I don't need to inoculate a lot of jars right now. I just want to get good mycelium growth without contams.

I have to make my own syringes because of where I live. The spore print I'm working with is from a wild P. Cubensis.


--------------------
YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.


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Offlinexwave3000
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Re: Are these too dark [Re: Ekstaza]
    #1507927 - 04/30/03 04:08 PM (14 years, 1 month ago)

Right. My friend doesn't need 200 syringes. I think he has enough there. Thanks


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OfflineAmanita_Dreamer
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Re: Are these too dark [Re: Anno]
    #1507947 - 04/30/03 04:14 PM (14 years, 1 month ago)

Quote:

Absolute waste of spores.



YEP....what if one or all of your syringes are contaminated?????If you make ten insted of one odds are you have a better chance of a "streile spore syringe"IMO


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Edited by Amanita_Dreamer (04/30/03 04:18 PM)


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OfflineAnnoA
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Re: Are these too dark [Re: Ekstaza]
    #1507977 - 04/30/03 04:24 PM (14 years, 1 month ago)

>would it hurt to make syringes this dark.(i.e. contams more probable???)

Of course. The chance of contaminated syringe is greater if you put in the whole print.

Again, absolute waste, you could have easily made 10 syringes with this amount of spores.


Edited by Anno (04/30/03 04:42 PM)


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OfflineEkstaza
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Re: Are these too dark [Re: Anno]
    #1508022 - 04/30/03 04:39 PM (14 years, 1 month ago)

Thanks Anno,
This could be one of the reasons for my huge contamination problem. I also believe that the print being wild has something to do with it.


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YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.


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Offlinexwave3000
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Re: Are these too dark [Re: Ekstaza]
    #1508024 - 04/30/03 04:40 PM (14 years, 1 month ago)

but he just doesn't need anymore.


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InvisibleSixTango
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Re: Are these too dark [Re: Anno]
    #1508043 - 04/30/03 04:45 PM (14 years, 1 month ago)

Well well............. I never thought I would see the day that - I -, a grasshopper compared to ANNO would IN PART disagree wiyh him.

AGREE  it is a waste of spores.

However, one syringe made from one print - IMHO .................>

LOWERS  the odds of contamination..........IMHO.

Here is why. Say a spore print is contaminated. If you make 10 syringes from it & use them all to innoc spawn jars. You may end up with 50 quarts of contaminated spawn jars.

On the other hand, one syringe, into 5 jars limits that contamination to those 5 jars.

What my foaf now does is this.

He makes a quart of spore solution with several caps, in one of these.


:smile:


Once done, he  agitates the container, then withdraws a syringe & innocs several agar plates and/or & injects it into a few quart jars.

If  NONE show contam's while colonizing, he is then confident, he has a clean & viable spore solution in the 1/2 gallon container to draw on.

6T  :wink:
 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlinejakeholman
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Registered: 02/24/03
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Re: Are these too dark [Re: SixTango]
    #1508470 - 04/30/03 06:45 PM (14 years, 1 month ago)

well, 6T, your thoughts might be right, but it would make no difference wether you made 50 or 5 jars with your syringes, your percentage would still be the same, but only with more jars used-that was a bad example. but as long as you do the test that you recommend than it doesnt matter what you do because you are only risking a few jars as a test.
Jake H


--------------------
The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.


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OfflineAmanita_Dreamer
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Re: Are these too dark [Re: SixTango]
    #1508595 - 04/30/03 07:24 PM (14 years, 1 month ago)

Quote:

Well well............. I never thought I would see the day that - I -, a grasshopper compared to ANNO would IN PART disagree wiyh him.

AGREE  it is a waste of spores.

However, one syringe made from one print - IMHO .................>

LOWERS  the odds of contamination..........IMHO.

Here is why. Say a spore print is contaminated. If you make 10 syringes from it & use them all to innoc spawn jars. You may end up with 50 quarts of contaminated spawn jars.

On the other hand, one syringe, into 5 jars limits that contamination to those 5 jars.

What my foaf now does is this.

He makes a quart of spore solution with several caps, in one of these.


:smile:


Quote:

  Once done, he  agitates the container, then withdraws a syringe & innocs several agar plates and/or & injects it into a few quart jars.




Not everyone uses agar,or a 1/2 gallon container.In that case they could controll the variable by only innoc. 1 PINT from each syringe thus giving the user a definative answer to contams without losing too much..





Quote:

 
If  NONE show contam's while colonizing, he is then confident, he has a clean & viable spore solution in the 1/2 gallon container to draw on.

6T  :wink:
 


 

On the other hand if you put 'all your eggs in one basket' so to speak youve lost ALL OF THE 1/2 gallon.As oppsed to maybe only 4 out of 10 syringes being contaminated 


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OfflineSuntzu
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Re: Are these too dark [Re: xwave3000]
    #1509444 - 04/30/03 11:59 PM (14 years, 1 month ago)

Those syringes are so dark they might even throw off your nitrogen balance :wink:  Make sure to use an 18 gauge or larger needle.
I wonder if at a certain point there are enough spores in a solution to provide a nutrient base for bacteria [thus increased contam rates]. 

I'd bet that bacteria would be less likely to bloom in a dilute spore solution, anyway.

   


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Amazon Shop: ½ Pint Jars, Agar

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