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Invisibleatricoz
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Registered: 10/18/10
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Re: laetiporus sulphureus cultivation / agar [Re: BlackPeace]
    #17470229 - 12/30/12 09:42 AM (10 years, 5 months ago)

I guess this can be interesting. This summer in Austria I found these nice L. sulphureus specimens growing on a rotting fir stump.

+

I managed to clone it (pretty easy, since mycelium started to grow aggressively on agar just the day after) and prepared a spawn jar. Since I read somewhere that this species is a secondary decomposer (didn't manage to find again this information, though) as substrate, I used a spent Agrocybe aegerita block. I crushed, re-hydrated, supplemented with 10% bran and then sterilized. I stored the bag at room temp inside of a closet, checking it periodically. When it was fully colonized, mycelium started to climb bag's walls, and reached the filter. So I noticed what looked like a primordium, popping out through the filter. At first I though it was just mycelium, but the blob has the consistency of the mushroom, and really looks like a primordium.

This is how it looked at first



and here is how it looks currently







Now I cutted the bag's top, to let the climbing mycelium breathe, and I made some cuts on the bag. I'll let you know if it'll continue to grow :wink:


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OfflineC4G
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Re: laetiporus sulphureus cultivation / agar [Re: atricoz]
    #17470501 - 12/30/12 11:09 AM (10 years, 5 months ago)

:popcorn:


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Until next time...

            ------------
"Faith is a journey, not a guilt trip."
            ------------
"I don't disregard the power of faith but despise the contradictory nature of the mainstream beliefs that dare to claim a monopoly over morality."
            ------------
"If you don't ask the questions, how do you expect to find the answers?"

I WANT: Paddystraw WEDGE or LC /Trade List
        SILKWORM EGGS


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InvisibleSporulator
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Re: laetiporus sulphureus cultivation / agar [Re: C4G]
    #17470603 - 12/30/12 11:41 AM (10 years, 5 months ago)

It looks promising, keep us updated.  :thumbup:  :mushroom2:
Thanks for sharing!


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Edited by Sporulator (12/30/12 11:55 AM)


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OfflineBlackPeace
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Re: laetiporus sulphureus cultivation / agar [Re: Sporulator]
    #17471410 - 12/30/12 02:38 PM (10 years, 5 months ago)

Ohhhhhhh!


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OfflineC4G
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Re: laetiporus sulphureus cultivation / agar [Re: BlackPeace]
    #17480427 - 01/01/13 10:58 AM (10 years, 5 months ago)

I found some of Chicken in the Wood last summer as well. I have several agar dishes full of myc. I await eagerly your results. :chugbeer:


--------------------
Until next time...

            ------------
"Faith is a journey, not a guilt trip."
            ------------
"I don't disregard the power of faith but despise the contradictory nature of the mainstream beliefs that dare to claim a monopoly over morality."
            ------------
"If you don't ask the questions, how do you expect to find the answers?"

I WANT: Paddystraw WEDGE or LC /Trade List
        SILKWORM EGGS


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Invisibleatricoz
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Re: laetiporus sulphureus cultivation / agar [Re: C4G]
    #17487684 - 01/02/13 05:49 PM (10 years, 5 months ago)

That thing looks a lot like a primordium, now it's showing a more orange color, and it's continuing to grow. I'll post a pic in the next days


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OfflineBlackPeace
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Re: laetiporus sulphureus cultivation / agar [Re: atricoz]
    #17490449 - 01/03/13 08:08 AM (10 years, 5 months ago)

Thx for sharing, I'm very interested in this.


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Invisibleatricoz
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Re: laetiporus sulphureus cultivation / agar [Re: BlackPeace]
    #17506423 - 01/06/13 10:51 AM (10 years, 4 months ago)

Here's how it looks today. As I said, it started to look more orange. Plus, something is popping out from the cut I made on the sides of the bag. Pretty slow growing, though.





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InvisibleSporulator
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Re: laetiporus sulphureus cultivation / agar [Re: atricoz]
    #17507218 - 01/06/13 01:51 PM (10 years, 4 months ago)

Wow! It looks definitely like a fruiting body!  :mushroom2:  :thumbup:


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Offlinenycomyco
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Re: laetiporus sulphureus cultivation / agar [Re: atricoz]
    #17510994 - 01/07/13 08:25 AM (10 years, 4 months ago)

very interesting.  maybe try lowering humidity at this point.  chickens often grow in dry conditions- maybe because they pump so much liquid through the fruit.


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OfflineMycelio
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Re: laetiporus sulphureus cultivation / agar [Re: nycomyco]
    #17511286 - 01/07/13 09:59 AM (10 years, 4 months ago)

It's growing! I wouldn't risk anything by changing conditions.

Congratulations, Atricoz!
Misting might have done the trick.

Carsten


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Offlinewbastz
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Re: laetiporus sulphureus cultivation / agar [Re: atricoz]
    #17516173 - 01/08/13 08:06 AM (10 years, 4 months ago)

Hi atricoz,

Can you see pores on this primordium ?

Looks like you got something cool here!


Cheers,

Wilson


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Offlinenycomyco
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Re: laetiporus sulphureus cultivation / agar [Re: Mycelio]
    #17517734 - 01/08/13 02:50 PM (10 years, 4 months ago)

Mycelio, you're advice about not adjusting conditions is probably wise.  I just thought that lowering humidity might help since growth has slowed.  To me the primordial tissue looks a bit water-logged and I've never seen happy water-logged chickens in the wild.  at this stage of growth in the wild, the fruit is expanding rapidly and looks like a cumulus cloud.  Chickens can survive dry conditions- and often the best fruitings I've seen have come during very dry days following rains.  when you cut a chicken, it bleeds out sweet yellow fluid, so surely the primordia is fed plenty of water from the mycelial mass. 

My 2 cents would be that if this growth stalls, see if you can provide dryer conditions for any subsequent primordia that form in the new slits you cut. 

Best of luck, I'm watching this very closely, and great work so far!


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OfflineMycelio
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Re: laetiporus sulphureus cultivation / agar [Re: nycomyco]
    #17519398 - 01/08/13 08:15 PM (10 years, 4 months ago)

That's interesting, Nycomyco. I only found them once during a long rainy period. Maybe I took the exception for the rule and didn't think any further. Every fast growing mushroom must have to get rid of large amounts of water.
But then... every time, I see pics of L.s. fruiting in cultivation, I see water droplets from misting.
For Example here: 
http://galeria.grzybland.pl/main.php?g2_itemId=22917&g2_page=2

Carsten


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Invisibleatricoz
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Re: laetiporus sulphureus cultivation / agar [Re: Mycelio]
    #17521983 - 01/09/13 12:09 PM (10 years, 4 months ago)

unluckily I'm not at home now so I can't update the situation. I'll do as soon as I'll be back. In the meanwhile, thanks for your interest :smile:


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Offlinetrout
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Re: laetiporus sulphureus cultivation / agar [Re: atricoz]
    #17522596 - 01/09/13 02:16 PM (10 years, 4 months ago)

I had a bag do that in a dry garage and I moved it into a humid FC and it stalled and then molded. I moved it out and set it back in the garage and it attempted to make another blob but never developed as far as the first.  I would not drastically alter conditions but I would not increase h2o in any way.  Looks good!!! All the picture of lab fruited L.sulp. are from the filter. Keep it up we are about to master this one!!!

Trout


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I need tropical cultures, ABM, V.v....!!!!

Well things don't always look as they are and things can be misread and mistaken for what they realy are so don't read too much into what I say since I might be mistaken myself. And remember I rarely ever give a definate answer.


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Offlinewbastz
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Re: laetiporus sulphureus cultivation / agar [Re: trout]
    #17531308 - 01/11/13 04:48 AM (10 years, 4 months ago)

Hello,

This is very interesting! Perhaps the combination of a depleted substrates  together with the use of filters (or even tiny pinholes made in the plastic bag) are a good match for this species of mushroom.

Another species of mushroom that grows well from tiny pinholes is Hericium erinasceus. Has anyone seen a fruiting of H.erinasceus growing directly on  the filter of a plastic bag ?  I never seen it .

Just curiosity !

Cheers ,

Wilson


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Offlinet3chnobily
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Re: laetiporus sulphureus cultivation / agar [Re: wbastz]
    #17531402 - 01/11/13 06:12 AM (10 years, 4 months ago)

I had a forgotten bag of lions mane climb up and fruit out of the patch.  Didn't fully develop due to extreme dryness but it was trying.


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OfflineKadoka
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Re: laetiporus sulphureus cultivation / agar [Re: t3chnobily]
    #17543207 - 01/13/13 04:21 PM (10 years, 4 months ago)

I found these; I was thinking of trying to make a cardboard spawn clone







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Offlinetrout
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Re: laetiporus sulphureus cultivation / agar [Re: Kadoka]
    #17543575 - 01/13/13 05:46 PM (10 years, 4 months ago)

That last pic is fing beautiful. I want to spend a day sitting on that log pondering the cultivation of this mushroom.


--------------------
I need tropical cultures, ABM, V.v....!!!!

Well things don't always look as they are and things can be misread and mistaken for what they realy are so don't read too much into what I say since I might be mistaken myself. And remember I rarely ever give a definate answer.


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