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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 12 years, 2 months
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inoculation loop sterilization
#15040430 - 09/06/11 10:39 PM (12 years, 5 months ago) |
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I am kind of worried that I could kill spores if I flame sterilize my inoculation loop and then use it to quickly before it has cooled down. If I sterilize my inoculation loop with an alcohol lamp outside of my glove box and then wipe my loop on an alcohol pad once it gets inside the glove box would it work to cool the loop enough? Or would this just be overkill? ...Gawd so many questions as I'm just about to get started
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SexySmurff
HyperQube



Registered: 06/21/04
Posts: 522
Loc: HyperSpace
Last seen: 3 years, 10 months
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Re: inoculation loop sterilization [Re: 760Rollz]
#15040452 - 09/06/11 10:45 PM (12 years, 5 months ago) |
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you're actually going backwards by doing that. alcohol doesn't sterilize it sanitizes.
-------------------- Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.
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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 12 years, 2 months
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Re: inoculation loop sterilization [Re: SexySmurff]
#15040552 - 09/06/11 11:16 PM (12 years, 5 months ago) |
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So then it wouldn't be over kill? So it would cool the loop down enough to use? Or can I just move from flame, to box, to print and make a syringe without worrying about destroying living spores because the loop has not had enough time to cool down?
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hoobatech

Registered: 08/16/04
Posts: 285
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Re: inoculation loop sterilization [Re: 760Rollz]
#15040565 - 09/06/11 11:19 PM (12 years, 5 months ago) |
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the wire is really thin, just hold it for 20 sec or however long it takes to cool to the touch
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Meetzu
G̙̗͙̞̮͖̥a͉̭̹r̝̹͖̬d̞̮̻̪en̫̭e̱r̼



Registered: 11/22/10
Posts: 432
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Re: inoculation loop sterilization [Re: 760Rollz]
#15040568 - 09/06/11 11:21 PM (12 years, 5 months ago) |
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Quote:
760Rollz said: So then it wouldn't be over kill? So it would cool the loop down enough to use? Or can I just move from flame, to box, to print and make a syringe without worrying about destroying living spores because the loop has not had enough time to cool down?
He is saying it's not something you should do because alcohol does not kill mold spores but heat does.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: inoculation loop sterilization [Re: Meetzu]
#15040736 - 09/07/11 12:32 AM (12 years, 5 months ago) |
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heat the loop red hot, then cool it by sticking it into the agar of the receiving dish for a few seconds.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 5 days
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Re: inoculation loop sterilization [Re: sandman420]
#15041493 - 09/07/11 07:44 AM (12 years, 5 months ago) |
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Quote:
sandman420 said: heat the loop red hot, then cool it by sticking it into the agar of the receiving dish for a few seconds.
This is the correct procedure. Wiping a loop with alcohol after flame sterilizing is like wiping shit on your face after taking a shower. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Doctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc:
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Re: inoculation loop sterilization [Re: RogerRabbit]
#15041624 - 09/07/11 08:42 AM (12 years, 5 months ago) |
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Quote:
RogerRabbit said:
Quote:
sandman420 said: heat the loop red hot, then cool it by sticking it into the agar of the receiving dish for a few seconds.
This is the correct procedure. Wiping a loop with alcohol after flame sterilizing is like wiping shit on your face after taking a shower. RR
Damn dude! 


DI
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steelmonkey
Homejigger



Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada
Last seen: 2 years, 5 months
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Re: inoculation loop sterilization [Re: Doctor_Inoc]
#15041782 - 09/07/11 09:22 AM (12 years, 5 months ago) |
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Very graphic,but it made me laugh all the same
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 6 months, 5 days
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Re: inoculation loop sterilization [Re: steelmonkey]
#15042259 - 09/07/11 11:21 AM (12 years, 5 months ago) |
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RR cracks me up!
-------------------- My Old Grow Logs
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LayinUp
Crush yo rating like yo butthole



Registered: 04/14/11
Posts: 2,232
Loc: Between the permafrost an...
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Re: inoculation loop sterilization [Re: RogerRabbit]
#15042320 - 09/07/11 11:31 AM (12 years, 5 months ago) |
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Quote:
RogerRabbit said: ...wiping shit on your face after taking a shower. RR
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Escape the box.
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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 12 years, 2 months
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Re: inoculation loop sterilization [Re: LayinUp]
#15054382 - 09/09/11 05:22 PM (12 years, 5 months ago) |
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lol Thanks all.
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