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Offlinebigj2
journeyman
Registered: 03/23/03
Posts: 55
Last seen: 13 years, 4 months
Spore syringe question
    #1501957 - 04/28/03 09:44 PM (13 years, 7 months ago)

I just recieved 4 syringes in the mail. The syringes came with needles, but they are not attached. (They are in sealed packs seperatly) Because the needles are not attatched, I was wondering if the plastic tip where the needles slide on to the syringe is going to be prone to contamination. (there is no cover on it) Every other syringes i've every gotten came with the needles in place so sterilization was as simple as heating the needle. Let me know.


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Offlinefugu
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Registered: 01/06/03
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Re: Spore syringe question [Re: bigj2]
    #1501968 - 04/28/03 09:47 PM (13 years, 7 months ago)

mail needle ss people help him
>so sterilization was as simple as heating the needle
yes


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Offlinebigj2
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Registered: 03/23/03
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Re: Spore syringe question [Re: fugu]
    #1501980 - 04/28/03 09:49 PM (13 years, 7 months ago)

What??? I asked if the platic tip of the needle was prone to contamination sine I recieved the syringes without needles attatched.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
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Re: Spore syringe question [Re: bigj2]
    #1501997 - 04/28/03 09:53 PM (13 years, 7 months ago)

In a clean enviro, (alc swab hands first) - tear open the big end of the needle package without withdrawning the needle from the cover, pull cap off syringe & twist the needle attatchment part onto the syringe. Then pull off needle cover when you are about to use the syringe.

That be that ................6T  :wink:


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleZwieback0
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Registered: 08/23/02
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Re: Spore syringe question [Re: bigj2]
    #1502000 - 04/28/03 09:54 PM (13 years, 7 months ago)

No, it wont be. Just attach the tip to the needle, wrap it in foil, and follow the sterilization tek for syringes in the FAQ.


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Offlinebigj2
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Registered: 03/23/03
Posts: 55
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Re: Spore syringe question [Re: Zwieback0]
    #1502020 - 04/28/03 09:57 PM (13 years, 7 months ago)

Wait, My fault... I meant to ask if the plastic tip of the Syringe was prone to contams because the needle isnt attatched. This is where the spores will exit the syringe.


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InvisibleZwieback0
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Re: Spore syringe question [Re: bigj2]
    #1502047 - 04/28/03 10:02 PM (13 years, 7 months ago)

no


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Offlinebigj2
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Registered: 03/23/03
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Re: Spore syringe question [Re: Zwieback0]
    #1502110 - 04/28/03 10:16 PM (13 years, 7 months ago)

That seems weird to me. Seems it would be prone to contams.


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InvisibleSixTango
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Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Spore syringe question [Re: bigj2]
    #1502139 - 04/28/03 10:22 PM (13 years, 7 months ago)

Syringes made that way were made with an IV valve & the solution was drawn from a large container. Once drawn into the syringe, the tip is swabbed & capped.

That is about as clean as you can get.

If you are tweaky about it, pull the syringe tip cap off & alc swab the tip, before adding the needle.

Thats that.............6T :tongue:


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Edited by SixTango (04/28/03 10:23 PM)


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OfflineDank420
Shrooms...Mmm

Registered: 04/12/03
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Re: Spore syringe question [Re: bigj2]
    #1502151 - 04/28/03 10:24 PM (13 years, 7 months ago)

Where exactly is the sterilization tek for syringes in the FAQ. I can't seem to find it.


--------------------
"I tried marijuana once. I did not inhale." -William Clinton.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Spore syringe question [Re: Dank420]
    #1502235 - 04/28/03 10:43 PM (13 years, 7 months ago)

Just wash old syringes out, rinse, wrap in tinfoil & PC 20 minutes.

If you don't have a PC? Push the plunger all the way in, then spray some lysol down the plunger tube, from the plunger end. Boil some water (rolling boil) & draw in into the syringe, squirt out & repeat several times.

Alc swab some tinfoil, wrap then up & store in a clean autoclave bag, or zip lock.

6T.


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineDank420
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Registered: 04/12/03
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Re: Spore syringe question [Re: SixTango]
    #1502245 - 04/28/03 10:46 PM (13 years, 7 months ago)

Cool thanks 6T, so out of the box, the spore syringes are steril enough to use right? This post got me thinking.


--------------------
"I tried marijuana once. I did not inhale." -William Clinton.


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OfflineMike Elium
.the Mycelium

Registered: 02/04/03
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Re: Spore syringe question [Re: bigj2]
    #1502250 - 04/28/03 10:47 PM (13 years, 7 months ago)

Isn't there a sterile plug in the end of the syringe, which twists out, then the steriile needle goes on ?


--------------------
your inside is out, and your outside is in.


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InvisibleTrippinSLIM
Cosmonaut

Registered: 04/27/03
Posts: 42
Re: Spore syringe question [Re: Mike Elium]
    #1502268 - 04/28/03 10:52 PM (13 years, 7 months ago)

sterilize it anyway with alcohol wipes


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Spore syringe question [Re: Dank420]
    #1502300 - 04/28/03 11:00 PM (13 years, 7 months ago)

Quote:

Cool thanks 6T, so out of the box, the spore syringes are steril enough to use right?  This post got me thinking. 




Alc swab the syringe tip -- before adding the needle. You cannot sterilize a loaded spore syringe (anyway) without killing the spores -- INSIDE............... :grin:

6T


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlinebigj2
journeyman
Registered: 03/23/03
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Last seen: 13 years, 4 months
Re: Spore syringe question [Re: SixTango]
    #1502311 - 04/28/03 11:03 PM (13 years, 7 months ago)

No, there was no cover over the tip of the syringe. I have been afraid to dab the tip with alcohol for fear of killing some of the spores.


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Offlineebola8p
Stranger
Registered: 03/03/03
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Re: Spore syringe question [Re: bigj2]
    #1502350 - 04/28/03 11:11 PM (13 years, 7 months ago)

Quote:

No, there was no cover over the tip of the syringe. I have been afraid to dab the tip with alcohol for fear of killing some of the spores.




so the syringes are full? and they came with no needle attached?


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InvisibleZwieback0
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Re: Spore syringe question [Re: ebola8p]
    #1502978 - 04/29/03 02:16 AM (13 years, 7 months ago)

I have heard you can just boil water, pull water into the syringe and squeeze it out is enough to sterilzie it.


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
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Re: Spore syringe question [Re: Zwieback0]
    #1502996 - 04/29/03 02:23 AM (13 years, 7 months ago)

Guys....I had 2 syringes arrive in the mail a while back...luer slip....I hate those things....anyway they were not well packed and the needle, tips and all broke, I used a second set of syringes to draw the remaining inocculum out of the others and had used the same prep method 6T had suggested....both syringes were saved....



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OfflineMike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
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Last seen: 1 year, 2 months
Re: Spore syringe question [Re: bigj2]
    #1505700 - 04/29/03 08:52 PM (13 years, 7 months ago)

Bigj2,

Not to beat this to death, but I'm to the point where I SIMPLY MUST KNOW..... my impression is that you got syringes that were ALREADY LOADED WITH SPORES IN SOLUTION......right? Because all of the answers you are getting are good, but I can see that some people think you got unloaded syringes, but others (like me) think you got loaded ones. I've seen loaded ones come from one particularly reputable vendor, always with the needles off, and it always APPEARS that there is nothing on the end of the syringe, but there IS a plug that twists out, and which you replace with the needle..... Otherwise, your solution would have spilled out during shipment. When you say you were afraid to dab the tip w alcohol for fear of killing spores, I again concluded that it was a loaded syringe, with spores that could in fact be killed. IF SO, I'm willing to bet it only APPEARS like there is nothing on the end.....but there IS in fact a plug.

So, are my deductions Sherlock Holmes-like, or am I just an idiot (only Bigj2 may reply the latter) ? And if a vendor DID send you loaded syringes with the needles off and no plug, don't ever buy from him again.



--------------------
your inside is out, and your outside is in.


Edited by Mike Elium (04/29/03 08:53 PM)


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