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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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OfflineCosmicShame
Enlightened One
Registered: 04/12/03
Posts: 13
Last seen: 13 years, 20 days
Contamination or no????
    #1498356 - 04/27/03 06:46 PM (13 years, 7 months ago)

Here is a picture of possible contamination. It only covers a very small portion of the jar and doesn't appear to be "dangerous." However I am new at this and I need everyone's opinion as to what this is and if it can be grown safely.

Thanks!!!

Here it is



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Anonymous

Re: Contamination or no???? [Re: CosmicShame]
    #1498502 - 04/27/03 07:48 PM (13 years, 7 months ago)

Hard to see.
What color is it?
If it's not white be careful.
Looks almost reddish.
May be lipstick mold.
Let it sit around, if it spreads, chuck out the jar.
When it doubt, chuck it out!

Keep us updated.

Also: Make sure you check Contamination FAQ and see if it matches up to anything located there.
Rule of thumb: Check first, then ask.

Also: If it doesn't seem to spread, and the jar is 100% colonized, open it up and smell it. If it smells un-mushroomy, throw it out, probably infestated with contamination anyway. If smells shroomy, go and birth it.

Hope that helped.
Now must do physics.
Bye.


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OfflineCosmicShame
Enlightened One
Registered: 04/12/03
Posts: 13
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Re: Contamination or no???? [Re: daba]
    #1498547 - 04/27/03 08:14 PM (13 years, 7 months ago)

It is a reddish-brown color, almost like rust. It hasn't spread like most of the pics of lipstick mold that I have seen. This cake has recently been placed in a chamber to fruit since the contamination never spread beyond that point.


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Offlineheadtrip
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Registered: 03/14/03
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Re: Contamination or no???? [Re: CosmicShame]
    #1498978 - 04/27/03 11:06 PM (13 years, 7 months ago)

I too have seen this same prob. before, from what i heard it stopped the growth in the jar. I posted before and nobody seemed to know what it was. I want to add too, i was a little suspicous of the smell when the incubator was opened, is there anything my friend could do when handling them, where a mask or someth'n???



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"its just like that, because thats the way it is"


Edited by headtrip (04/27/03 11:37 PM)


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OfflineJohnnyRespect
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Registered: 04/17/03
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Re: Contamination or no???? [Re: headtrip]
    #1501510 - 04/28/03 07:15 PM (13 years, 7 months ago)

Without seeing it closer up, i think it's what my iguana has. Brown rusty powder? See if it develops a white "border' around it, if so, thats what we got too



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As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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InvisibleZwieback0
Baby Bread
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Registered: 08/23/02
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Re: Contamination or no???? [Re: JohnnyRespect]
    #1501713 - 04/28/03 08:10 PM (13 years, 7 months ago)

You know what? How about taking a knife and cutting a big chunk out of that cake just to be on the safe side! GL


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Offlineheadtrip
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Registered: 03/14/03
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Re: Contamination or no???? [Re: Zwieback0]
    #1501828 - 04/28/03 08:54 PM (13 years, 7 months ago)

brown rusty powder with a white border, that would def. be it... its at the points of innoculation too.. i figured it came from the liquid mycelium my friend had used, i'll see if i can come up with a webcam to get some closer pics..


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"its just like that, because thats the way it is"


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OfflineJohnnyRespect
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Re: Contamination or no???? [Re: headtrip]
    #1506075 - 04/29/03 11:18 PM (13 years, 7 months ago)

Yea, brown rusty powder white fuzzy border same here.  With me, it appeared just below the verm layer (you had to almost angle it to see it) then in splotches all around the jar. fucking thing.  I'm considering taking it into the lab with me sometime and seeing just what the fuck it is.  Anyone have any idea? Rice endospores perhaps? how did you sterilize your jars, what did you use? Lets track this fucker down and eliminate it :smile:

Jr


--------------------
As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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OfflineOmegaRed
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Registered: 04/16/03
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Re: Contamination or no???? [Re: JohnnyRespect]
    #1507439 - 04/30/03 01:39 PM (13 years, 7 months ago)

I have this same thing on my cakes (MMGG, PC Eq.). I am also a first timer. The only difference that I have symptom-wise is that the healthy mushroom mycellium will sometimes grow right over the top of this stuff and then its gone. What the hell is going on here??? Is this a contam or no?


--------------------
Hey Mr. Tamborine Man play a song for me,
I'm not sleepy and there's no place I'm going to.
Hey Mr. Tamborine Man play a song for me,
In the jingle jangle morning I'll come following you.


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OfflineJohnnyRespect
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Re: Contamination or no???? [Re: OmegaRed]
    #1507556 - 04/30/03 02:27 PM (13 years, 7 months ago)

Everyone that has this type of contam, characterized by a brown-red rusty powder look that is surrounded by a whiteish cloud, please tell us all how you mixed your substrate for the jars, your sterilization time, and any other pertinent details relating to the jar prep / sterilization / storage.  I'll start :smile:

I use a mixture of 1 part ground up brown rice to 2 parts professional grade Sta-Green vermiculite.  I have four holes in the top of my jars, covered with construction grade metal duct tape (Not the "duct" tape that you usally buy, it's shiny thin metal stuf, I don't know why they named it the same as a product that already exists).  I put a layer of dry verm on top, as per the PF Tek.  I cover the tops of the jars with alum foil (reynolds wrap, the thickest kind I can find).  When I do my innoculations, I remove the alum foil, put it in the oven (I use an oven lid, i'm going to step up to an innoc hood soon).  I've noticed that this whateveritis usually appears just below the dry verm layer, maybe the dryness of the verm doesn't let it sterilize properly?  Also, my sterilization is done at 20 psi for an hour and a half (Thats 1.5 hours exposure time).

I'm not sure exactly what this is, perhaps some endospores left on the rice?  I read in a FAQ  that soaking the rice in cold water can activate the spores, making them easier to kill.  Has anyone here that has this infection ever tried this? Maybe that could be our solution.  Hopefully if we gather enough information about this little bugger, we can identify it's origin and eliminate it.

Peace
JR


--------------------
As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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OfflineAmanita_Dreamer
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Registered: 03/16/03
Posts: 330
Loc: The 666th Plane of HELL
Last seen: 13 years, 4 months
Re: Contamination or no???? [Re: JohnnyRespect]
    #1507726 - 04/30/03 03:14 PM (13 years, 7 months ago)

Hard to tell from the pic but it looks alot like invetro. From the pic those look to me anyways like pins.If it smells mushroomy when you sell it then it probably IS  pinns growing inside your jar.You describe them as being:

It is a reddish-brown color, almost like rust. It hasn't spread like most of the pics of lipstick mold that I have seen.

If they are cubiensis alot of cubies start with a reddish "rusty" colored cap(pin) getting lighter with age esp. ones like G.T.'s or creeper.Hope its pinning...if it is that cake is going to blow the fuck up!!!Good Luck :grin:

O.K.... I just looked closer sorry im comming off a 63gram trip. I see what your all talking about.It looks like mycellium "piss".After a jar is fully colonized, or one that is taking an unusually long time to reach 100% colonization  and left in the jar for a extended length of time like any living organisim, will expell waste. With this in mind and nowhere for this "waste" to go it will eventually pool in a rusty yellow inside the jar. If not taken out soon like anything living in it's own shit, it will get sick and die.So I say birth that puppy!!


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Edited by Amanita_Dreamer (04/30/03 03:24 PM)


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OfflineOmegaRed
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Re: Contamination or no???? [Re: Amanita_Dreamer]
    #1507903 - 04/30/03 04:03 PM (13 years, 7 months ago)

Ok, so here's my situation. I sterilized my jars 4 in a small pressure cooker, 8 in a pot of water for times sake. Blended BRF and Verm. as per MMGG. Aluminum foil over 4 holes in the lid as per the MMGG. The color appeared at the innoculation sites for a couple of the jars, but on others the mycellium grew for several days and then the color appears on the edges of the mycellium. I have one jar that has generated this color twice and the mycellium grew right back over it each time. But I also have other jars that have not even begun to show signs of growth and have this color around the innoculation sites.


--------------------
Hey Mr. Tamborine Man play a song for me,
I'm not sleepy and there's no place I'm going to.
Hey Mr. Tamborine Man play a song for me,
In the jingle jangle morning I'll come following you.


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OfflineAmanita_Dreamer
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Registered: 03/16/03
Posts: 330
Loc: The 666th Plane of HELL
Last seen: 13 years, 4 months
Re: Contamination or no???? [Re: OmegaRed]
    #1507972 - 04/30/03 04:23 PM (13 years, 7 months ago)

Mycellium "piss" - After a jar is fully colonized, or one that is taking an unusually long time to reach 100% colonization and left in the jar for a extended length of time like any living organisim, will expell waste. With this in mind and nowhere for this "waste" to go it will eventually pool in a rusty yellow inside the jar. If not taken out soon like anything living in it's own shit, it will get sick and die....Then again maybe it's an old syringe?


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Edited by Amanita_Dreamer (04/30/03 04:25 PM)


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OfflineJohnnyRespect
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Registered: 04/17/03
Posts: 676
Loc: East Coast
Last seen: 13 years, 5 months
Re: Contamination or no???? [Re: Amanita_Dreamer]
    #1509238 - 04/30/03 11:17 PM (13 years, 7 months ago)

It's not mycelia piss. It's a brown very dry powder. I opened one of them up and it's everywhere inside. It turns verm and rice into a powder. I thin kthis is going to require lab identification since noone seems t oknow wtf it is. I guarantee that what I have isn't mycelia piss. Some of the jars don't even have white mycelia in them.

Jr


--------------------
As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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Anonymous

Re: Contamination or no???? [Re: JohnnyRespect]
    #1509720 - 05/01/03 01:19 AM (13 years, 7 months ago)

maybe its some type of mold that the mushroom mycelium is able too kill easily


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Offlineheadtrip
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Registered: 03/14/03
Posts: 90
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Re: Contamination or no???? [Re: ]
    #1510170 - 05/01/03 03:23 AM (13 years, 7 months ago)

woah wait a second, how many of you used sta-green vermiculite, i have never had this problem until they started with their new version of it this year.... the verm. that comes pre-moistened if anybody knows of it???


--------------------
---------------------------------------------------------
"its just like that, because thats the way it is"


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OfflineOmegaRed
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Registered: 04/16/03
Posts: 193
Loc: right behind you
Last seen: 12 years, 8 months
Re: Contamination or no???? [Re: headtrip]
    #1512084 - 05/01/03 07:09 PM (13 years, 7 months ago)

No, I'm not using sta-green verm. I'm using some generic brand that was from the only source of verm in about a 60 mile radius.


--------------------
Hey Mr. Tamborine Man play a song for me,
I'm not sleepy and there's no place I'm going to.
Hey Mr. Tamborine Man play a song for me,
In the jingle jangle morning I'll come following you.


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OfflineJohnnyRespect
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Registered: 04/17/03
Posts: 676
Loc: East Coast
Last seen: 13 years, 5 months
Re: Contamination or no???? [Re: OmegaRed]
    #1515874 - 05/02/03 09:57 PM (13 years, 7 months ago)

I used sta-green.  Hmmm... are those fuckers fighting the war on drugs in their own way? :smile:

jr


--------------------
As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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OfflineJohnnyRespect
Nomadic Wanderer
Registered: 04/17/03
Posts: 676
Loc: East Coast
Last seen: 13 years, 5 months
Re: Contamination or no???? [Re: JohnnyRespect]
    #1516354 - 05/03/03 01:44 AM (13 years, 7 months ago)

This time my iguana is misting 4x a day with a 1:10 h2o2 / h2o mixture. He's a few days into trying to coax the pins out, and no infection yet (knocks on wood repeatedly)

Jr


--------------------
As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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Offlineheadtrip
&%$#@!!

Registered: 03/14/03
Posts: 90
Last seen: 12 years, 5 months
Re: Contamination or no???? [Re: JohnnyRespect]
    #1521874 - 05/05/03 02:53 PM (13 years, 7 months ago)

update to figure'n out what this contam is... my friend cleaned out all his jars that were infected by this, they seemed to smell like coconuts, didn't get too good of a smell from them, was a little cautious, could all be in his head but he said he was start'n to get short of breath and a headache while work'n around it... if anybody has any ideas on figure'n this contam out, nothing in the forum seems to apply to it..


--------------------
---------------------------------------------------------
"its just like that, because thats the way it is"


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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