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Offlinemadis503
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Registered: 07/29/09
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whens the last time to clone?
    #14966123 - 08/23/11 02:21 PM (12 years, 5 months ago)

hi! i need quick help. i had to move back to my parents apartement and so i had to move my shrooms to my friends place. now it appears the second flush is bringing me some good size shrooms(i had only tiny before) so i really need to make clones from these but the problem is that i can make it after 24hours and he picked them like yesterday. i told him to put them in the. fridge today. is it possible that i can take some shroom tissue and clone them tomorrow or are they useless already?
pic:


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Offlinemadis503
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Registered: 07/29/09
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Re: whens the last time to clone? [Re: madis503]
    #14966130 - 08/23/11 02:22 PM (12 years, 5 months ago)

i55.tinypic.com/n19pub.jpg


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Invisiblevirus1824
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Re: whens the last time to clone? [Re: madis503]
    #14966143 - 08/23/11 02:24 PM (12 years, 5 months ago)

You can clone on agar (obviously) and if it appears to contain additional unwanted molds, you can simply transfer the good mycelium to produce a clean isolate. So yes you can still clone. Keep them in a fridge until ready to start procedure.


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Offlinemadis503
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Re: whens the last time to clone? [Re: virus1824]
    #14966181 - 08/23/11 02:33 PM (12 years, 5 months ago)

ok thanks for the quick answer... hope ill find some agar tomorrow i have no idea where to get it and petri dishes also, any suggestions? i thouht of makeing lc.. im not in us so in what kind of stores is agar sold?


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OfflineWing
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Re: whens the last time to clone? [Re: madis503]
    #14966192 - 08/23/11 02:35 PM (12 years, 5 months ago)

Agar can be found in asian markets or health food stores. Possibly in a regular grocery store.


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Invisiblevirus1824
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Re: whens the last time to clone? [Re: madis503]
    #14966209 - 08/23/11 02:37 PM (12 years, 5 months ago)

most probably asian food markets as the gentleman above me mentioned. I wouldn't waste time on starting a LC from that. the chances of it containing contaminations are very high, and you will just end up with a bad LC. You don't want that.

PS you can use very small jars as petri dishes. Check Doc-T posts for a review on that technique.


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Offlinemadis503
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Re: whens the last time to clone? [Re: virus1824]
    #14966233 - 08/23/11 02:43 PM (12 years, 5 months ago)

ok thanks ill hope i find these :smile:


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Offlinemadis503
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Re: whens the last time to clone? [Re: madis503]
    #14969529 - 08/24/11 05:37 AM (12 years, 5 months ago)

1 more question: if i clone with agar is my only choice to put it in grain?


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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: whens the last time to clone? [Re: madis503]
    #14969550 - 08/24/11 05:56 AM (12 years, 5 months ago)



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InvisibleBarakanaten
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Re: whens the last time to clone? [Re: Carl Sagan]
    #14969605 - 08/24/11 06:23 AM (12 years, 5 months ago)

this will get you started with agar. Grocery Store Agar Tek


There are some examples on many different styles of lids in my Eazy Felt Tek

Easy Agar Dishes

petri Jars 
I use the same configuration for Petri Jars as I do for Spawn Jars, 

 

sometimes I use Plastic Jars too, its just a deep petri to me.
drill a 1/2 hole dead center slap an Easy Disk on an BANG! 



the glass jars were $4 and the plastic was $2 the easy felt is $1-2$

depending on price and availability in your area you could
easily get set up for agar + dishes for $10 r so:super:

hope this helps you.


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OfflineeLeSDenes
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Re: whens the last time to clone? [Re: Barakanaten]
    #14969675 - 08/24/11 06:51 AM (12 years, 5 months ago)

because of the 2nd flush gives you some good sized mushroom it does not mean if you clone them you'll get the same sized mushrooms. After you dunk the cakes the mushrooms grow bigger. But you can clone them though they are nice!


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InvisibleFungus_monk
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Re: whens the last time to clone? [Re: eLeSDenes]
    #14969681 - 08/24/11 06:53 AM (12 years, 5 months ago)

Quote:

eLeSDenes said:
because of the 2nd flush gives you some good sized mushroom it does not mean if you clone them you'll get the same sized mushrooms.



what?
a cloned mushroom has the exact same genetic make-up as the mushroom you cloned from, so yes, you would get the same sized mushrooms as long as its enviroment stayed the same aswell.


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InvisibleBarakanaten
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Re: whens the last time to clone? [Re: eLeSDenes]
    #14969689 - 08/24/11 06:57 AM (12 years, 5 months ago)

Cloning will also give you better flushes and most likely a better pinset and a more even canopy.

clone from the middle of clusters and from small pins when you can.

the idea being that younger tissue is better.


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InvisibleBarakanaten
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Re: whens the last time to clone? [Re: Fungus_monk]
    #14969709 - 08/24/11 07:07 AM (12 years, 5 months ago)

Quote:

eLeSDenes said:
because of the 2nd flush gives you some good sized mushroom it does not mean if you clone them you'll get the same sized mushrooms.




Correct!



Quote:

Fungus_monk said:

Quote:

eLeSDenes said:
because of the 2nd flush gives you some good sized mushroom it does not mean if you clone them you'll get the same sized mushrooms.






what?
a cloned mushroom has the exact same genetic make-up as the mushroom you cloned from, so yes, you would get the same sized mushrooms as long as its enviroment stayed the same aswell.




Incorrect, the torn up landscape of a sub after the first flush would minimize desirable
pinning spots and that could lead to fewer fruits but much larger than the original cloned fruit.



:themoreyouknow:


--------------------
:mushroomgrow:New to cultivation? :sunny: Have a Question?:mushroomgrow:
Because AMU We Q&A Know
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  AMU
:blueangel:
Official PNW Mushroom Hunting Season 2011
"...Though in all else he may be as Saintly as St. Francis, in the pursuit of these ends he is more
Satanic than Satan. He will betray his nearest and dearest without the slightest twitch of flesh or spirit.
He is amoral."
-Definition of a mushroom hunter by ~Angelo Pellegrini
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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
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Re: whens the last time to clone? [Re: Fungus_monk]
    #14969725 - 08/24/11 07:16 AM (12 years, 5 months ago)

Quote:

Fungus_monk said:
Quote:

eLeSDenes said:
because of the 2nd flush gives you some good sized mushroom it does not mean if you clone them you'll get the same sized mushrooms.



what?
a cloned mushroom has the exact same genetic make-up as the mushroom you cloned from, so yes, you would get the same sized mushrooms as long as its enviroment stayed the same aswell.





Quote:

a cloned mushroom has the exact same genetic make-up as the mushroom you cloned




True.

Quote:

you would get the same sized mushrooms




False

Quote:

as long as its enviroment stayed the same aswell.




Like the post above stated second, or third flushes, will generally produce larger fruits.

Even the size of the fist flush, comparatively to the first flush of the cloned culture,
Has a number of factors that will determine fruit size.

IME, one of the biggest factors is pin set.
I have had ridiculous pin sets that produce allot of smallish mushrooms on a first flush.
I have also had clones, not mono-cultures, that produce Ok pin sets, but the fruits are much larger.


I understand that you stated "as long as its enviroment stayed the same aswell"
and yes those environmental conditions will effect pin set, essentially "reproducing"
the same size fruits.

So you're partially correct, but the idea that a second flush monster, will always produce
that size fruits, doesn't jive.  It's highly probable that it will produce those fruits on the second flush.

I hope this makes sense:lol:

Dam Barak, you beat me to it!:lol:


--------------------
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Edited by Carl Sagan (08/24/11 07:17 AM)


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InvisibleFungus_monk
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Re: whens the last time to clone? [Re: Carl Sagan]
    #14969742 - 08/24/11 07:29 AM (12 years, 5 months ago)

Thank you Carl Sagan and Barakanaten, i didnt know that. You learn something new everyday around here.


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InvisibleBarakanaten
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Re: whens the last time to clone? [Re: Fungus_monk]
    #14969745 - 08/24/11 07:30 AM (12 years, 5 months ago)

Quote:

Carl Sagan said:


Dam Barak, you beat me to it!:lol:





:datass:


Quote:

Fungus_monk said:
Thank you Carl Sagan and Barakanaten, i didnt know that. You learn something new everyday around here.





No worries bro, thats were here for:headbang3:


--------------------
:mushroomgrow:New to cultivation? :sunny: Have a Question?:mushroomgrow:
Because AMU We Q&A Know
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Official PNW Mushroom Hunting Season 2011
"...Though in all else he may be as Saintly as St. Francis, in the pursuit of these ends he is more
Satanic than Satan. He will betray his nearest and dearest without the slightest twitch of flesh or spirit.
He is amoral."
-Definition of a mushroom hunter by ~Angelo Pellegrini
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OfflinePsilomega84
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Registered: 06/15/10
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Re: whens the last time to clone? [Re: Carl Sagan]
    #14969756 - 08/24/11 07:37 AM (12 years, 5 months ago)

single fruits carry multiple strains unless grown from an already isolated myc from spores on agar.


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Offlinemadis503
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Re: whens the last time to clone? [Re: Psilomega84]
    #14969963 - 08/24/11 08:49 AM (12 years, 5 months ago)

yeah i know second flush gives bigger shrooms but these are like totally different from what ive had before.
previous grows:

now:

some previous 2nd flush:


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