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OfflineThisIsPathetic
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Interesting Liquid Culture... NEVER seen before!
    #14954615 - 08/20/11 10:46 PM (13 years, 4 months ago)

So I posted back awhile ago about some LCs that I made, and got interesting results...

There seemed to be a 'white island' floating on top of my LC... and a lot of the responses to it said that it was contamed, would turn green within a couple days, and that I should toss them... well... I kept them anyways.

Its now been a couple months... and the results seem to have... well... Take a look :smile:

(Link to what they looked like in the beginning)



I actually tested the LC out a couple days ago on some BRF Cakes... We shall see how they do, but here are a two of the jars that I took some LC out of for the cakes





Here is one of the jars that I actually opened as a 'test' to see if fresh air did anything, and to get a clearer picture. Took a decent 'whiff' of it and it smelled like PURE Mycelium:





Here are two syringes that I made a while back... around when I posted about this the first time (see link above). I kept them in a tyvek envelope in a Rubbermaid bin for storage... Pulled them out the pother day and noticed this:





Notice the 'growth' wherever the LC meets with the air... It's not contamed, I don't believe, since it smells like Mycelium... but at the same time... I have never seen a liquid culture start to 'form' when it met air. One of the syringes had a big air bubble in it (oops) but even the other airtight syringe... it still found a TINY bit of air by the needle to 'grow'.


Anyone ever encounter this? Interested in hearing some logic from everyone on what is happening... why it's happening... or if maybe I stumbled upon something new when it comes to Liquid Culture o.O I dunno... I'm hoping for some kind of 'super strain' :smile: <- yeah right.


Feedback always welcome... really interested to hear from the community and hopefully some of the pros out there :laugh:


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Now that's what I call cubes :smile:



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InvisibleAdmiral Ackbar
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #14954655 - 08/20/11 10:54 PM (13 years, 4 months ago)

It happens. If you have enough gas exchange in the jar, I have had the myc produce small fruits and spoorulate on the island in LC jars.


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I'll tell your ass when it is a mother fucking trap!


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OfflineThisIsPathetic
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Re: Interesting Liquid Culture... NEVER seen before! [Re: Admiral Ackbar]
    #14954666 - 08/20/11 10:56 PM (13 years, 4 months ago)

Also looking for a LC tek to use on a new batch of LCs...

I like the idea of using the Light Malt Extract that I have and the Dextrose...

(anyone) know any good formulas?? (Quart Size Jars)


--------------------
Now that's what I call cubes :smile:



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Invisiblemaug
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #14954678 - 08/20/11 10:59 PM (13 years, 4 months ago)

It kinda sounds like they colonized over a yeast colony. Very neat tho =)

And from what I hear...
"Use 1 g of elme per 600ml of water" (elme is extra light malt extract)


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I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes

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InvisibleUnnamedGrower
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Re: Interesting Liquid Culture... NEVER seen before! [Re: maug]
    #14954683 - 08/20/11 11:00 PM (13 years, 4 months ago)

Quote:

ThisIsPathetic said:
Also looking for a LC tek to use on a new batch of LCs...

I like the idea of using the Light Malt Extract that I have and the Dextrose...

(anyone) know any good formulas?? (Quart Size Jars)



1 gram of ELME per 600ml of water no dextrose works awesome for me
Quote:

maug said:
It kinda sounds like they colonized over a yeast colony. Very neat tho =)

And from what I hear...
"Use 1 g of elme per 600ml of water" (elme is extra light malt extract)



Where did you hear this from man???:lmafo:

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Invisiblesandman420
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #14954684 - 08/20/11 11:00 PM (13 years, 4 months ago)

yea man you cant beat damien5050s "elementary lc tek"


Off the top of my head I think it is 1/4 cup dex, 1 teaspoon malt and 2 quarts of water, enough for 4 quart jars of LC.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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OfflineThisIsPathetic
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Re: Interesting Liquid Culture... NEVER seen before! [Re: sandman420]
    #14954720 - 08/20/11 11:09 PM (13 years, 4 months ago)

Quote:

sandman420 said:
yea man you cant beat damien5050s "elementary lc tek"


Off the top of my head I think it is 1/4 cup dex, 1 teaspoon malt and 2 quarts of water, enough for 4 quart jars of LC.




Since the last batch was 1tbsp-(E)LME/1tbsp-Dextrose I think I'm going to go with just the (E)LME this time lol... I don't need anymore islands!

but thanks for the advice everyone... It's that quick info I was lookin for :laugh: and now it's time to make me some NEW LCs


--------------------
Now that's what I call cubes :smile:



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OfflineThisIsPathetic
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Re: Interesting Liquid Culture... NEVER seen before! [Re: UnnamedGrower]
    #14954741 - 08/20/11 11:17 PM (13 years, 4 months ago)

UnnamedGrower said:
Quote:

ThisIsPathetic said:
Also looking for a LC tek to use on a new batch of LCs...

I like the idea of using the Light Malt Extract that I have and the Dextrose...

(anyone) know any good formulas?? (Quart Size Jars)



1 gram of ELME per 600ml of water no dextrose works awesome for me




Hmm, the previous recipe called for using 1tbsp of each per jar if I recall correctly... Just weighed out a tbsp of LME and it comes out to over 10 grams! That's a huge difference... and maybe why my LC is soo 'growy'?


--------------------
Now that's what I call cubes :smile:



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InvisibleUnnamedGrower
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #14954752 - 08/20/11 11:22 PM (13 years, 4 months ago)

Quote:

ThisIsPathetic said:
UnnamedGrower said:
Quote:

ThisIsPathetic said:
Also looking for a LC tek to use on a new batch of LCs...

I like the idea of using the Light Malt Extract that I have and the Dextrose...

(anyone) know any good formulas?? (Quart Size Jars)



1 gram of ELME per 600ml of water no dextrose works awesome for me




Hmm, the previous recipe called for using 1tbsp of each per jar if I recall correctly... Just weighed out a tbsp of LME and it comes out to over 10 grams! That's a huge difference... and maybe why my LC is soo 'growy'?



This is transcending life's LC recipe and I swear by it.  Works fucking awesome IME.  Im usually ready to shoot a test jar in 10 days following this

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OfflineThisIsPathetic
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Re: Interesting Liquid Culture... NEVER seen before! [Re: UnnamedGrower]
    #14954786 - 08/20/11 11:32 PM (13 years, 4 months ago)

Quote:

UnnamedGrower said:
Quote:

ThisIsPathetic said:
UnnamedGrower said:
Quote:

ThisIsPathetic said:
Also looking for a LC tek to use on a new batch of LCs...

I like the idea of using the Light Malt Extract that I have and the Dextrose...

(anyone) know any good formulas?? (Quart Size Jars)



1 gram of ELME per 600ml of water no dextrose works awesome for me




Hmm, the previous recipe called for using 1tbsp of each per jar if I recall correctly... Just weighed out a tbsp of LME and it comes out to over 10 grams! That's a huge difference... and maybe why my LC is soo 'growy'?



This is transcending life's LC recipe and I swear by it.  Works fucking awesome IME.  Im usually ready to shoot a test jar in 10 days following this





I'll take your word. Just measured out 2400ml of water, and I'm about to measure out 4g of (E)LME...

Bring the water to a boil, add the malt, boil/stir for a couple minutes, then filter out the sediment, load up 4 quart jars, PC @ 15 PSI for 15 mins, let cool, inoculate? Sound about right?


--------------------
Now that's what I call cubes :smile:



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InvisibleUnnamedGrower
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #14954843 - 08/20/11 11:49 PM (13 years, 4 months ago)

Yea except no need to filter with ELME IME

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InvisibleHorizonSpawn
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Re: Interesting Liquid Culture... NEVER seen before! [Re: UnnamedGrower]
    #14954898 - 08/21/11 12:08 AM (13 years, 4 months ago)

Pics remind me of my homebrew kombucha :smile:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

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OfflineThisIsPathetic
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Re: Interesting Liquid Culture... NEVER seen before! [Re: HorizonSpawn]
    #14954907 - 08/21/11 12:12 AM (13 years, 4 months ago)

Quote:

HorizonSpawn said:
Pics remind me of my homebrew kombucha :smile:




?? Elaborate :smile:


--------------------
Now that's what I call cubes :smile:



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Offlinek00laid
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #14955044 - 08/21/11 01:06 AM (13 years, 4 months ago)

Quote:

ThisIsPathetic said:
Here is one of the jars that I actually opened as a 'test' to see if fresh air did anything, and to get a clearer picture. Took a decent 'whiff' of it and it smelled like PURE Mycelium:







that sure as hell doesnt look like cubensis mycelium to me, but i guess i dont have much experience with sugar LC


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InvisibleHorizonSpawn
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Re: Interesting Liquid Culture... NEVER seen before! [Re: k00laid]
    #14955133 - 08/21/11 01:37 AM (13 years, 4 months ago)

Kombucha is a live culture drink :smile:

Try making some; it is a very healthy life-force restorative beverage.


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

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Invisiblemaug
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Re: Interesting Liquid Culture... NEVER seen before! [Re: UnnamedGrower]
    #14957068 - 08/21/11 02:41 PM (13 years, 4 months ago)

Quote:

UnnamedGrower said:
1 gram of ELME per 600ml of water no dextrose works awesome for me
Quote:

maug said:
And from what I hear...
"Use 1 g of elme per 600ml of water" (elme is extra light malt extract)



Where did you hear this from man???:lmafo:




The mushrooms told me. They talk... O_o


--------------------
I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes

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InvisibleUnnamedGrower
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Re: Interesting Liquid Culture... NEVER seen before! [Re: maug]
    #14957120 - 08/21/11 02:50 PM (13 years, 4 months ago)

Quote:

maug said:
Quote:

UnnamedGrower said:
1 gram of ELME per 600ml of water no dextrose works awesome for me
Quote:

maug said:
And from what I hear...
"Use 1 g of elme per 600ml of water" (elme is extra light malt extract)



Where did you hear this from man???:lmafo:




The mushrooms told me. They talk... O_o



Dam to you too:highfive: I thought I was special and that they only talked to me:shrug:

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Offlineely2121
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Re: Interesting Liquid Culture... NEVER seen before! [Re: UnnamedGrower]
    #14959177 - 08/22/11 12:12 AM (13 years, 4 months ago)

Love this thread :smile:

I have a bunch of LME/DEX LCS that have been sitting for a couple months after I switched to GLC and promptly forgot about them.

Totally reminded me of Kombucha too!  It's like they are forming a little SCOBY on top.
I would really like to see if some of them will form little lily pads with little fruits on top. Might make me buy a macro lens for the gf haha.

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OfflineCheeseBurgler
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ely2121]
    #14959802 - 08/22/11 06:47 AM (13 years, 4 months ago)

i use TL's LC tek everytime, and some get islands, and some don't, and it has nothing to do with contams. for example


its not very big, but thems some islands. i just stir them up with the stirrer; sometimes they break up, sometimes they dont. old, new, filtered, unfiltered...its a fucking draw of the hat.

i HAVE had the EXACT same thing happen in a homebrew MS jar, with fruits and everything, just chillin on top of the myc island. pictures soon, even if i have to resurrect this thread. :biggrin:


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OfflineThisIsPathetic
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Re: Interesting Liquid Culture... NEVER seen before! [Re: CheeseBurgler]
    #15169659 - 10/02/11 11:30 PM (13 years, 3 months ago)

Quote:

YouKnowEdDavis said:
i use TL's LC tek everytime, and some get islands, and some don't, and it has nothing to do with contams. for example


its not very big, but thems some islands. i just stir them up with the stirrer; sometimes they break up, sometimes they dont. old, new, filtered, unfiltered...its a fucking draw of the hat.

i HAVE had the EXACT same thing happen in a homebrew MS jar, with fruits and everything, just chillin on top of the myc island. pictures soon, even if i have to resurrect this thread. :biggrin:




STILL WAITING... I wanna see island fruits, AND YES, THIS TRULY IS RELEVANT :smile:


--------------------
Now that's what I call cubes :smile:



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Invisiblewildernessjunkie
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Re: Interesting Liquid Culture... NEVER seen before! [Re: ThisIsPathetic]
    #15169774 - 10/03/11 12:11 AM (13 years, 3 months ago)

:popcorn:

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