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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Cobweb prevention?
#14945160 - 08/18/11 11:49 PM (12 years, 7 months ago) |
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So i posted this in contam forum with not much activity. I have searched but couldn't find much on prevention.
So just check my jars, had 10 lost 7 all to cobweb. What precautions can i take to fight off getting a cobweb contam? so far i have lost all jars except 3 to damned cobweb. got a fresh batch sterilizing as we speak.
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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VaeVictum
Sativa Cyborg
Registered: 06/01/11
Posts: 4,397
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Make a glove box and develop a better sterile technique?
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Re: Cobweb prevention? [Re: VaeVictum]
#14945351 - 08/19/11 12:44 AM (12 years, 7 months ago) |
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got me drop sheets for my dexter syle room, just need the timber.
whats best type of construction for this 'tent' in relation to the frame design?
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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dr harry bush
Grow wiz kid
Registered: 03/19/11
Posts: 322
Loc: 3rd rock from the sun
Last seen: 11 years, 9 months
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hi Nox overview your sterile technique. i used to have bad problems with contam and thought i was doing everything right. i sat down and assessed my sterile technique and noticed i had picked up one or two overlooked bad habits. i then went back to basics to relearn techniques by the book and slowly adding my own flair again. my success rate is now much better. i get about 2% contam rate on grows. 100% success on agar,grain,g2g,lc's etc...
hope this helps peace
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Well i redone my 7 jars today.
used a different room to innoc this time. Cleaned it all down and its alot smaller. used different source of spores for each innoc and left 2 just sterile to see if its my substrate/sterilization process.
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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babypp2
Stranger
Registered: 06/29/11
Posts: 196
Last seen: 11 years, 1 month
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whats best type of construction for this 'tent' in relation to the frame design?
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Re: Cobweb prevention? [Re: babypp2]
#14945700 - 08/19/11 03:21 AM (12 years, 7 months ago) |
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There could be many rectangle, square, A frame, circular, hexagonal etc.
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Use a glovebox and sterile procedure as shown in part 2 of the Brown Rice Flour tek.
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Having trouble finding a tote that seals fairly well. And gloves that are suitable and nimble enough to use.
On a budget of sorts.\
How could i fix these totse that arnt fully locked? Polyfill than clamp shut?
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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HorizonSpawn
Gettin' my grow on :)
Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org
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I use mine upside down... Meaning; the bottom of the lid is used as my work surface and the bin is placed over what I'm working on...
Some have done so with the bin on its side and placed their arm holes through the lid.
-------------------- Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC... NOTE: Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Damn it, one contam already!
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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Carl Sagan
Time Dilation Analyst
Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
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Quote:
had 10 lost 7 all to cobweb.
I would be suspect of your syringe, along with reviewing your aseptic tek.
You are over thinking the GB.
Get a 105qt tub, and a 6" hole saw, and your done.
Just use disposable latex gloves, and clean the box before use. Wipe down each jar, and tool as you put it in.
-------------------- “Sacred cows make the best hamburger” Mark Twain Independant Research Foundation
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Its not the syringes, freshie every jar. I make my own maybe thats my problem in that process. They are not prints, but the mushroom caps themselves are my source.
So a GB doesn't have to be airtight?
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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Fungus_monk
Here or there...
Registered: 08/07/11
Posts: 1,589
Loc: U.S.
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I use a cooler on its side with the lid open, so mine definitly isnt.
-------------------- My Trade List I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Ahh k, will go buy myself a tote.
Quote:
Wild samples, alot of different sources. 150ml cups. Verm, Manure and BRF with foil folded twice to achieve 4 layers. This is done twice so there is 2 foil lids that are 4 layers thick. Half inch of dry verm as barrier now been increased to 1 inch.
Boiled/Steamed in pot for at least 2 hours. everything gets a alcho wipedown, hands washed with antibacterial soap. bleach and glen twenty sprayed in air an left for 5 mins.
Innoced in my kitchen after a thorough cleaning with bleach, now in a pantry of sorts that got the same treatment with twice the bleach content.
No glove box yet or PC because i went to purchase one that had been there for ages at big discount but was sold day before and only one they had was with no gauge.
Gonna hit OP shops and 2nd hand electrical an furniture.
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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Carl Sagan
Time Dilation Analyst
Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
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Quote:
They are not prints, but the mushroom caps themselves are my source.
Explain this process of inoculation.
-------------------- “Sacred cows make the best hamburger” Mark Twain Independant Research Foundation
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Scape the gills with a knife that was boiled/bleached/alch wipe an flame off, into a spoon that had same treatment as knife. Sterile water that was done while boiling jars. Open syringe suck up solution minus big 'bits' Remove top lid wipe needle with alch and injection site. done for every injection. replace top lid
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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Carl Sagan
Time Dilation Analyst
Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
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Quote:
NoxADVANCED said: Scape the gills with a knife that was boiled/bleached/alch wipe an flame off, into a spoon that had same treatment as knife. Sterile water that was done while boiling jars. Open syringe suck up solution minus big 'bits' Remove top lid wipe needle with alch and injection site. done for every injection. replace top lid
This sounds like where you may be having a problem.
Why not just print the caps under a glass?
I PC my syringes in qt jars.
Swabs are best done to agar, as it's easier to spot contamination.
These are BRF cakes..... correct??
And you are following the tek perfectly?
Dry verm layer and all?
Where are you getting these caps? In door cake......... or wild?
-------------------- “Sacred cows make the best hamburger” Mark Twain Independant Research Foundation
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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Quote:
NoxADVANCED said: Ahh k, will go buy myself a tote.
Quote:
Wild samples, alot of different sources. 150ml cups. Verm, Manure and BRF with foil folded twice to achieve 4 layers. This is done twice so there is 2 foil lids that are 4 layers thick. Half inch of dry verm as barrier now been increased to 1 inch.
Boiled/Steamed in pot for at least 2 hours. everything gets a alcho wipedown, hands washed with antibacterial soap. bleach and glen twenty sprayed in air an left for 5 mins.
Innoced in my kitchen after a thorough cleaning with bleach, now in a pantry of sorts that got the same treatment with twice the bleach content.
No glove box yet or PC because i went to purchase one that had been there for ages at big discount but was sold day before and only one they had was with no gauge.
Gonna hit OP shops and 2nd hand electrical an furniture.
Waiting on agar supplies through pharmacist. Dried samples i have picked,ID'd and tripped balls!
Basically im trying to fruit them to get clean prints to share trade and fruit myself. If i happen to get a trip dose from them yay!
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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NoxADVANCED
Research & Information Collector
Registered: 05/05/06
Posts: 6,872
Loc: Straya Cunt
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I believe it is my spores but will continue until march april i can get cleaner prints.
-------------------- Ethno-Garden: My seeds and Herbs My Contests/Give-aways Get on it! WE DID IT for kratom I have my rating privileges back, BITCHES
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