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InvisibleJoshua
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Best Antibiotic for Spore Syringes?
    #1495013 - 04/26/03 03:11 AM (13 years, 7 months ago)

I have access to:

Kanamycin
Gentamycin
Ampicillin

Which should I use for my azure syringes?

Joshua


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Offlinehomebrew
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1495663 - 04/26/03 01:13 PM (13 years, 7 months ago)

I don't really think it should matter too much unless you plan on autoclaving the antibiotic/h2o prior to adding the spores. Gentamycin can be autoclaved without thermally degrading. Personally, I would go for the cheapest.


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InvisibleJoshua
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Re: Best Antibiotic for Spore Syringes? [Re: homebrew]
    #1496122 - 04/26/03 04:57 PM (13 years, 7 months ago)

I suppose I could look up which one has the broadest spectrum of activity...anyone want to save me the time?

Joshua


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Offlinehomebrew
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1497170 - 04/27/03 02:34 AM (13 years, 7 months ago)

Abbreviated Info:

Product : USE : Mode of action : working concentration

Ampicillin: gram +/- bacteria: interferes with cell wall formation: 50 ug/ml

Gentamycin: mycoplasma, gram +/- : 30S subunit of ribosome : 15 ug/ml

Kanamycin: mycoplasma, gram +/- : 70S subunit of ribosome : 30 ug/ml



Pulled From:

www.scimart.com/chemicals/AMRESCO/ 7-SciMart%20Amresco%20Cell%20Culture%20and%20Media%20Section.pdf


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OfflineOOKLA
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Re: Best Antibiotic for Spore Syringes? *DELETED* [Re: Joshua]
    #1499518 - 04/28/03 02:00 AM (13 years, 7 months ago)

Post deleted by OOKLA


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OfflineJohnnyRespect
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Re: Best Antibiotic for Spore Syringes? [Re: OOKLA]
    #1501042 - 04/28/03 04:59 PM (13 years, 7 months ago)

If you can't autoclave the one that you think would be ideall, see about other methods of sterilization.  Sterrad machines, for example, work great on sensitive materials, tho I'm not sure about liquid?  ETO might work, but I'm not sure.  Also it's really bad for ya :smile:

JR


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OfflineRaadt
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Re: Best Antibiotic for Spore Syringes? [Re: JohnnyRespect]
    #1501060 - 04/28/03 05:04 PM (13 years, 7 months ago)

I don't think he'll be autoclaving a spore syringe :wink:


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InvisibleJoshua
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Re: Best Antibiotic for Spore Syringes? [Re: Raadt]
    #1502786 - 04/29/03 01:01 AM (13 years, 7 months ago)

Do you think I should sterilize my syringes before or after I put the spores in?

Joshua


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Offlinedebianlinux
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1503948 - 04/29/03 12:28 PM (13 years, 7 months ago)

lol!


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OfflineRaadt
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1505566 - 04/29/03 08:15 PM (13 years, 7 months ago)

lol, depends if you want the antibiotic to breakdown  :grin:


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OfflineAnnoA
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1505594 - 04/29/03 08:22 PM (13 years, 7 months ago)

Why don?t you strike the spores on antibiotic agar?


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InvisibleJoshua
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Re: Best Antibiotic for Spore Syringes? [Re: Anno]
    #1505639 - 04/29/03 08:35 PM (13 years, 7 months ago)

Because I cannot send cultures in the mail. I can send syringes. I want to suspend the spores in solution so I can distribute them to those who are interested. I have provided prints in the past, but was not able to make enough from this past season.

Joshua


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OfflineAnnoA
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1505706 - 04/29/03 08:54 PM (13 years, 7 months ago)

ic....

well, then gentamycin.


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InvisibleJoshua
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Re: Best Antibiotic for Spore Syringes? [Re: Anno]
    #1505743 - 04/29/03 09:06 PM (13 years, 7 months ago)

What is your reasoning? I am curious.

Joshua


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"Life After Death is Saprophytic!"


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OfflineAnnoA
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1506766 - 04/30/03 05:43 AM (13 years, 7 months ago)

It?s proven to survive sterilization, it?s widely used and tested.


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OfflineRaadt
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Re: Best Antibiotic for Spore Syringes? [Re: Anno]
    #1507181 - 04/30/03 11:52 AM (13 years, 7 months ago)

but he's not sterilizing it. He just wants to add some to a spore syringe. Josh, do you have all 3 currently? or are just able to get all 3? maybe it's worth trying 1 syringe of each. And innoculating some jars. Before you waste any prints.


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineAnnoA
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Re: Best Antibiotic for Spore Syringes? [Re: Raadt]
    #1507215 - 04/30/03 12:03 PM (13 years, 7 months ago)

>innoculating

* inoculating

But does he know that the antibiotic is completely clean, free of mold spores?
I mean, If you?re not completely sure, then sterilizing would be a good thing, and if you sterilize, it?s a good thing if the antibiotic can stand the sterilization.


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OfflineRaadt
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Re: Best Antibiotic for Spore Syringes? [Re: Anno]
    #1507223 - 04/30/03 12:06 PM (13 years, 7 months ago)

that's absolutely true, i'm just used to the gent that I use, which is sterile.

If the gent isn't sterile packaged, then it would be good to sterilize water/antibiotic mixture 1st, and yes - then it would be best to use gent, so it doesn't breakdown. However, if he has sterile ampules or whatever - then it might still be good to go with gent, because it has been time tested in mushroom cultivation, i'm not sure if the others have?


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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InvisibleJoshua
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Re: Best Antibiotic for Spore Syringes? [Re: Anno]
    #1509700 - 05/01/03 01:14 AM (13 years, 7 months ago)

I have abiotic GS solution in sterile vials, and powdered kanamycin and ampicillin. I plan on puting the powdered forms into solution and filtering with a micron filter before use.

You all will be my experiment. Muah..ha..ha..Ha!!!

Joshua


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Great books for inquiring minds!

"Life After Death is Saprophytic!"


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OfflineNoLogos
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Re: Best Antibiotic for Spore Syringes? [Re: Joshua]
    #1512668 - 05/01/03 09:39 PM (13 years, 7 months ago)

For what it's worth, I have used and am using kanamycin with Hericium erinaceus, Pleurotus ostratus, P. eringii, and shiitake *cultures*. I tried amp, but it allowed a little bacteria growth. I'll try the gent someday....


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