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sepsis
stranger than you



Registered: 06/03/10
Posts: 85
Last seen: 7 months, 1 hour
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stalling monotubs
#14929246 - 08/15/11 11:33 PM (12 years, 6 months ago) |
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Hey guys,
I have 3 monotubs doing the spawn run thing as of about 2 1/2 weeks ago. My first thoughts are that it is taking way too long. It usually takes me 9 to 10 days to reach 100%. That said, I used a slightly larger tub than I usually do (27qts as opposed to 20qts.) My spawn to substrate ratio is 1:4. I am following Damion5050's tek, so I just used coir, gypsum, and verm. Usually I add a variety of other things, such as coffee, diatomaceous earth, and worm castings, but this time I decided to skip all those things as I have read from a lot of other posters that these things are unnecessary.
Anyway, when I checked on the tubs about a week in, they all seemed about 60-75% colonized. I thought it was going a little slow, but attributed this to the fact that it was ridiculously hot both in my house and outdoors (there was a heat wave that week). When I checked on them again about 3 days later, I expected they'd be close to 100%, but surprisingly there was no change.
I looked up other posts, and read that colonization may stall due to too much CO2 buildup, so I very quickly fanned out all three tubs. Now, about a week later, one of the tubs has finally reached 100% and looks really healthy. That one will be fruited. I am however concerned that the other 2 tubs are still stalled at around 75% although neither one looks or smells contaminated, in fact both smell sort of nice and mushroomy. I am also concerned that neither of these 2 tubs seem to want to colonize the top layer of coir/verm.
This isn't my first time doing monotubs, but I have never encountered this problem before. Is there anything I can do to fix this issue and rescue my tubs? I've also read that if the tubs are too wet (although I don't think these are too wet) it may slow things down. Is there anything that can be done if that's the problem? Any input on this would be most valued. Thanks!
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Barakanaten
Ama-gi



Registered: 04/14/10
Posts: 6,163
Loc: PNW Mycosphere( Blessed C...
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Re: stalling monotubs [Re: sepsis]
#14929368 - 08/16/11 12:17 AM (12 years, 6 months ago) |
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Pics would be awesome

your mono's may already be into fruiting stages due to the FAE you gave them and that may be why they are not colonizing anymore.
They may also be still colonizing slowly.
You may have to put the monos into fruiting.....
pics would help though.
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Slaithenn
Stranger

Registered: 05/19/10
Posts: 122
Last seen: 9 years, 10 months
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: stalling monotubs [Re: Slaithenn]
#14929547 - 08/16/11 01:16 AM (12 years, 6 months ago) |
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When this happens to me, I usually suspect spawn weakened by bacteria.
When mold is the culprit, then evidence is more obvious.
Good luck,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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Dudester67
eat a dick win a pony
Registered: 05/01/09
Posts: 632
Last seen: 12 years, 20 days
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Re: stalling monotubs [Re: Javadog]
#14929873 - 08/16/11 04:56 AM (12 years, 6 months ago) |
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Well, there's nothing much you can do but wait and see what happens. In the future you may want to adjust that spawn ratio to 1:2 or 1:3 for quicker colonization.
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gagaganja
Stranger

Registered: 01/15/11
Posts: 58
Last seen: 8 years, 8 months
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Re: stalling monotubs *DELETED* [Re: Dudester67]
#14930370 - 08/16/11 08:00 AM (12 years, 6 months ago) |
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Post deleted by gagaganjaReason for deletion: b
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sepsis
stranger than you



Registered: 06/03/10
Posts: 85
Last seen: 7 months, 1 hour
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Re: stalling monotubs [Re: gagaganja]
#14935177 - 08/17/11 12:42 AM (12 years, 6 months ago) |
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Quote:
Barakanaten said: your mono's may already be into fruiting stages due to the FAE you gave them and that may be why they are not colonizing anymore.
They may also be still colonizing slowly.
You may have to put the monos into fruiting.....
pics would help though.
I really only opened the tubs for a few seconds because they were colonizing so slowly, actually the fanning did help the colonization along, but only by another 5-10%. I don't think they are trying to fruit.
As for pics, I'd really rather not open up the tubs again for photos, but they really don't look at all out of the ordinary, just some 2/3 to 3/4 colonized tubs. Really not much to look at. No signs of contams...at most they're possibly too wet.
By the way, if they are too wet, can anything be done?
Quote:
Javadog said: When this happens to me, I usually suspect spawn weakened by bacteria.
When mold is the culprit, then evidence is more obvious.
Good luck,
JD
Yeah I had thoughts along these lines, but I always thought bacterial contamination smells really bad. Are there cases when it doesn't? The tubs don't only not smell bad, they even smell nice and shroomy.
Quote:
gagaganja said: how well did you break up the spawn i was in a hurry once and let some big chunks and i stalled out on me and what the two above said too
I guess I had some of the spawn pretty well broken up, but some of it was a little on the chunky side too. I wasn't aware that this could cause stalling. I'll be sure to break it up more finely next time. Thanks!
By they way, I was also thinking that the pasteurization method may be contributing to the issues with stalling. I normally pasteurize everything in jars in a PC, with a thermometer (RR's tek). This method hasn't ever failed me, but my oh my it is time consuming. Since I was doing a bunch of larger tubs, I decided to pasteurize as per Damion5050's bucket tek, which I had never done before. The coir seemed possibly a little wetter than I'm normally used to (although I tried to squeeze out the excess before putting it in the tubs). Also parts of the coir seemed cooler or hotter than the rest. Perhaps these factors are to blame?
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Dudester67
eat a dick win a pony
Registered: 05/01/09
Posts: 632
Last seen: 12 years, 20 days
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Re: stalling monotubs [Re: sepsis]
#14935484 - 08/17/11 02:23 AM (12 years, 6 months ago) |
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sepsis, i too was thinking you may have prepped your coir on the wet side which is not uncommon when using that damien bucket tek. Mycelium will have a tough time colonizing through a bulk sub if parts of the sub are too wet.
But tell me, are you using transparent tubs? Or you just basing these percentages of colonization on what you see on the surface? In my opinion it is essential to use clear, see-through tubs so you can inspect all sides of your substrate. If not, the bottom could be completely soaked and uncolonized and you'd never even know it.
But regardless, at 2.5 weeks after spawning you shouldn't be so reluctant to remove the lids and give those tubs some air. More air should do them good. But keep you spirits up, cubensis mycelium desperately WISHES to fruit and reproduce and create some jolly mushrooms for you. Based on the info you've given so far I predict you will start to see some knotting/pinning action within a week, at least on the frontrunner tub.
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sepsis
stranger than you



Registered: 06/03/10
Posts: 85
Last seen: 7 months, 1 hour
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Just an update: I've figured out what's wrong.
I have trich! Yikes! The mean green strikes again.
Too bad. Now from what I understand, if the tub contaminate before the first flush, then the issue is with the spawn. This makes sense as I waited a bit long to do the spawn run. I guess the only thing to do now is bury the the tub contents outdoors.
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