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InvisibleNoxADVANCED
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Cobweb prevention?
    #14945160 - 08/19/11 01:49 AM (9 years, 8 months ago)

So i posted this in contam forum with not much activity. I have searched but couldn't find much on prevention.

So just check my jars, had 10 lost 7 all to cobweb.
What precautions can i take to fight off getting a  cobweb contam? so far i have lost all jars except 3 to damned cobweb.
got a fresh batch sterilizing as we speak.


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InvisibleVaeVictum
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14945238 - 08/19/11 02:18 AM (9 years, 8 months ago)

Make a glove box and develop a better sterile technique? :shrug:


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: VaeVictum]
    #14945351 - 08/19/11 02:44 AM (9 years, 8 months ago)

got me drop sheets for my dexter syle room, just need the timber.

whats best type of construction for this 'tent' in relation to the frame design?


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Offlinedr harry bush
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14945601 - 08/19/11 04:22 AM (9 years, 8 months ago)

hi Nox overview your sterile technique. i used to have bad problems with contam and thought i was doing everything right. i sat down and assessed my sterile technique and noticed i had picked up one or two overlooked bad habits. i then went back to basics to relearn techniques by the book and slowly adding my own flair again.
my success rate is now much better. i get about 2% contam rate on grows. 100% success on agar,grain,g2g,lc's etc...

hope this helps
peace


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: dr harry bush]
    #14945615 - 08/19/11 04:32 AM (9 years, 8 months ago)

Well i redone my 7 jars today.

used a different room to innoc this time. Cleaned it all down and its alot smaller.
used different source of spores for each innoc and left 2 just sterile to see if its my substrate/sterilization process.


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Offlinebabypp2
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14945692 - 08/19/11 05:19 AM (9 years, 8 months ago)

whats best type of construction for this 'tent' in relation to the frame design?


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: babypp2]
    #14945700 - 08/19/11 05:21 AM (9 years, 8 months ago)

There could be many rectangle, square, A frame, circular, hexagonal etc.


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OfflineRogerRabbitM
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14946114 - 08/19/11 09:13 AM (9 years, 8 months ago)

Use a glovebox and sterile procedure as shown in part 2 of the Brown Rice Flour tek.


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: RogerRabbit]
    #14946154 - 08/19/11 09:27 AM (9 years, 8 months ago)

Having trouble finding a tote that seals fairly well. And gloves that are suitable and nimble enough to use.

On a budget of sorts.\

How could i fix these totse that arnt fully locked? Polyfill than clamp shut?


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InvisibleHorizonSpawn
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14946301 - 08/19/11 10:27 AM (9 years, 8 months ago)

I use mine upside down... Meaning; the bottom of the lid is used as my work surface and the bin is placed over what I'm working on...

Some have done so with the bin on its side and placed their arm holes through the lid.


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: HorizonSpawn]
    #14953854 - 08/20/11 09:21 PM (9 years, 8 months ago)

Damn it, one contam already!


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InvisibleCarl Sagan
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14953889 - 08/20/11 09:30 PM (9 years, 8 months ago)

Quote:

had 10 lost 7 all to cobweb.




I would be suspect of your syringe, along with reviewing your aseptic tek.

You are over thinking the GB.

Get a 105qt tub, and a 6" hole saw, and your done.

Just use disposable latex gloves, and clean the box before use.
Wipe down each jar, and tool as you put it in.


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: Carl Sagan]
    #14953924 - 08/20/11 09:38 PM (9 years, 8 months ago)

Its not the syringes, freshie every jar. I make my own maybe thats my problem in that process. They are not prints, but the mushroom caps themselves are my source.

So a GB doesn't have to be airtight?


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InvisibleFungus_monk
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14953929 - 08/20/11 09:39 PM (9 years, 8 months ago)

I use a cooler on its side with the lid open, so mine definitly isnt.


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14953946 - 08/20/11 09:44 PM (9 years, 8 months ago)

Ahh k, will go buy myself a tote.

Quote:


Wild samples, alot of different sources.
150ml cups. Verm, Manure and BRF with foil folded twice to achieve 4 layers. This is done twice so there is 2 foil lids that are 4 layers thick.
Half inch of dry verm as barrier now been increased to 1 inch.

Boiled/Steamed in pot for at least 2 hours.
everything gets a alcho wipedown, hands washed with antibacterial soap.
bleach and glen twenty sprayed in air an left for 5 mins.

Innoced in my kitchen after a thorough cleaning with bleach, now in a pantry of sorts that got the same treatment with twice the bleach content.




No glove box yet or PC because i went to purchase one that had been there for ages at big discount but was sold day before and only one they had was with no gauge.

Gonna hit OP shops and 2nd hand electrical an furniture.


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InvisibleCarl Sagan
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Registered: 04/19/11
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14953977 - 08/20/11 09:48 PM (9 years, 8 months ago)

Quote:

They are not prints, but the mushroom caps themselves are my source.




Explain this process of inoculation.


--------------------
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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: Carl Sagan]
    #14954051 - 08/20/11 10:06 PM (9 years, 8 months ago)

Scape the gills with a knife that was boiled/bleached/alch wipe an flame off, into a spoon that had same treatment as knife.
Sterile water that was done while boiling jars.
Open syringe suck up solution minus big 'bits'
Remove top lid
wipe needle with alch and injection site. done for every injection.
replace top lid


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InvisibleCarl Sagan
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14954080 - 08/20/11 10:16 PM (9 years, 8 months ago)

Quote:

NoxADVANCED said:
Scape the gills with a knife that was boiled/bleached/alch wipe an flame off, into a spoon that had same treatment as knife.
Sterile water that was done while boiling jars.
Open syringe suck up solution minus big 'bits'
Remove top lid
wipe needle with alch and injection site. done for every injection.
replace top lid




This sounds like where you may be having a problem.

Why not just print the caps under a glass?

I PC my syringes in qt jars.

Swabs are best done to agar, as it's easier to spot contamination.

These are BRF cakes..... correct??

And you are following the tek perfectly?

Dry verm layer and all?

Where are you getting these caps?  In door cake......... or wild?


--------------------
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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14954104 - 08/20/11 10:21 PM (9 years, 8 months ago)

Quote:

NoxADVANCED said:
Ahh k, will go buy myself a tote.

Quote:


Wild samples, alot of different sources.
150ml cups. Verm, Manure and BRF with foil folded twice to achieve 4 layers. This is done twice so there is 2 foil lids that are 4 layers thick.
Half inch of dry verm as barrier now been increased to 1 inch.

Boiled/Steamed in pot for at least 2 hours.
everything gets a alcho wipedown, hands washed with antibacterial soap.
bleach and glen twenty sprayed in air an left for 5 mins.

Innoced in my kitchen after a thorough cleaning with bleach, now in a pantry of sorts that got the same treatment with twice the bleach content.




No glove box yet or PC because i went to purchase one that had been there for ages at big discount but was sold day before and only one they had was with no gauge.

Gonna hit OP shops and 2nd hand electrical an furniture.




Waiting on agar supplies through pharmacist.
Dried samples i have picked,ID'd and tripped balls!

Basically im trying to fruit them to get clean prints to share trade and fruit myself. If i happen to get a trip dose from them yay!


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InvisibleNoxADVANCED
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Re: Cobweb prevention? [Re: NoxADVANCED]
    #14954116 - 08/20/11 10:25 PM (9 years, 8 months ago)

I believe it is my spores but will continue until march april i can get cleaner prints.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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