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Selected Test Results on the Alkaloid Content
of Conocybe cyanopus (% of Dry Weight)

Mushroom, Dry Weight(mg), Psilocybin, Baeocystin
1, 5, 0.84%, 0.15%
2, 6, 0.73%, 0.12%
3, 7, 1.01%, 0.20%
4, 10, 0.91%, 0.16%
5, 12, 0.89%, 0.14%

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I don't have enough yet. I want close communication with this one.

When I trip with liberty caps (which I rarely do) I want to go deep in to it, so about 4-6g = 100-150 liberty caps.

That means about the same amount in weight with  the conocybes, but not in numbers because they are tiny in comparison when dried.

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knarkkorven said:
Alan: I don't know, I don't have a microscope.

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maynardjameskeenan said:
Mine were also found growing in wood chips. Maybe it's a better habitat for them then book authors realize.

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psylosymonreturns said:

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maynardjameskeenan said:
Mine were also found growing in wood chips. Maybe it's a better habitat for them then book authors realize.

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maybe they are C smithii then.

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They might be!?! But just from the looks of them I would say cyanopus. I don't own a microscope so I guess is all just speculation.
From the looks of wisco's smithii finds they are not the same mushroom. Although morphology can be a bitch.
I think this is cyanopus

And this is smithii

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knarkkorven said:
Most grow in the grass in the middle of the tracks, but some are in the area where the grass meets the tracks, look at the habitat photo.

The Psilocybe semilanceata behaves in the same way, also grows like crazy (laaarge numbers) in the middle and also to the line where the wood chip starts.

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psylosymonreturns said:


hey Cactu, my Conocybe cyanopus started fruiting last year much earlier than other actives in the field!! my first obvervation was as early as July!!! i then found them in 4 other spots throughout the summer and into the fall.

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knarkkorven said:

cactu: Yes, earlier start, but I don't know when it ends. Will report back about that.

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Alan Rockefeller said:
http://mushroomobserver.org/obs/88084

Conclusion:  The bluing Conocybe species have been transferred into Pholiotina.  Spore size matches Pholiotina smithii better than P. cyanopus. BLAST shows  that the closest DNA match is 85% similar to Conocybe coprophila.

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fux234 said:
Great work Alan, but what is it now?

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knarkkorven said:
Weird result though. I don't think they macroscopically look like the smithii found in PNW more like the cyanopus.

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But you are a better judge, I almost don't know anything about spore features. But I believe this would be the first find of Conocybe smithii (=pholiotina smithii, damn I hate name changes ) in Sweden!

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But is 85% good enough to say it's c. coprophila or is the missing 15% an uncertainty big enough to suggest it to be a non yet described/DNA-sequenced species?

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Conocybe coprophila seems to be hard to find photos of.

These might be right?
http://virtualmycota.landcareresearch.co.nz/webforms/vM_Species_Details.aspx?pk=38461

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maynardjameskeenan said:

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Alan Rockefeller said:
http://mushroomobserver.org/obs/88084

Conclusion:  The bluing Conocybe species have been transferred into Pholiotina.  Spore size matches Pholiotina smithii better than P. cyanopus. BLAST shows  that the closest DNA match is 85% similar to Conocybe coprophila.

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Does this mean that all active conocybes fit better in the genus Pholiotina, or just smithii?

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Might be the first time that Knarkkorven has been mentioned on a US government website.  : )

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Yes, I only think I have been linked to from a EMCDDA report about psilocybin mushrooms before

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This collection is only 5 nucleotides different from http://mushroomobserver.org/134561, a maynardjameskeenan collection from Lebanon, Oregon.  Whether that means it's the same species or different is a matter of opinion, but it's super close in any case.

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This is interesting. Wasn't there like 3 differences between P. cyanescens and P. allenii when you described it as a new species?

I found the bluing Conocybe on the same spot for the following 3 years, but haven't found any recently. The ground around the tracks was pretty muddy from cows walking there one summer and then the farmer filled it up with gravel. I have also searched a lot of other habitat never found any more. It seems to be very rare.

In 2010 (one year before the Conocybe find) the same place looked like this:



It was a very special habitat... Good memories!

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knarkkorven said:


In 2010 (one year before the Conocybe find) the same place looked like this:



It was a very special habitat... Good memories!

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Really digging that middle shot.

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A very interesting and rare species in our dataset is Pholiotina cyanopus, for which we
analyzed three fruiting bodies from a single fungarium collection. P. cyanopus contained
0.821–1.360 mg/g BA, 0.247–0.565 mg/g NB, less than or equal to 0.062 mg/g PS, and less
than or equal to 0.859 mg/g PSB. Our results were very similar to the previous study by
Halama et al. [13], who also reported a trace concentration of AE.
https://doi.org/10.3390/ijms232214068

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