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Offlinefleshofgods
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peroxide and mycelium question
    #1487413 - 04/23/03 10:20 PM (13 years, 8 months ago)

I read the following here: http://www.shroomery.org/index/par/23534

Quote:

The jar is pressure cooked to sterilize and also destroy peroxide decomposing enzymes. When cool, the quart is injected with 12 cc?s peroxide, shaken and inoculated in front of a flow hood with a piece of the peroxidated agar.




I thought that a 3% peroxide solution would kill mycelium. Would this method work?


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Invisiblewoodrow
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Re: peroxide and mycelium question [Re: fleshofgods]
    #1487760 - 04/24/03 12:33 AM (13 years, 8 months ago)

I see no reason why this would not work. The quart jar contains a great deal of moisture in the form of soaked grain so the 12 cc of peroxide would be diluted to a safe level by the grain before it had time to do its damage. This should work for a mycelium inoculation but I would not expect it to work for spores.


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InvisibleJoshua
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Re: peroxide and mycelium question [Re: fleshofgods]
    #1487813 - 04/24/03 12:56 AM (13 years, 8 months ago)

I have tried the proceedure with success. As woodrow explained, the peroxide is diluted once injected into the grain jar.

Joshua


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Offlinecanid
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Re: peroxide and mycelium question [Re: Joshua]
    #1488264 - 04/24/03 03:52 AM (13 years, 8 months ago)

this method, though with less volume of %7 peroxide so as not to upset the moisture ratio too much, can also be used after inoculation, such as might be done to hedge odds against an outbreak of a particualr contam in the incubator.

[note: if used in the above scenario it may, in theory, kill some of the mycelium present before dispersion of the H2O2 into solution with the water present, so shaking is nesecary.]


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InvisibleJoshua
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Re: peroxide and mycelium question [Re: canid]
    #1488281 - 04/24/03 04:00 AM (13 years, 8 months ago)

I would also suggest lowering the temp in conjunction with your treatment as bacteria grow slower at lower temps.

Joshua


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Offlinefleshofgods
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Re: peroxide and mycelium question [Re: Joshua]
    #1489022 - 04/24/03 12:45 PM (13 years, 8 months ago)

thanks guys, i guess i was thinking more of spores being killed from the peroxide. but i guess i could see it working with a myc agar culture


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InvisibleTrippinSLIM
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Registered: 04/27/03
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Re: peroxide and mycelium question [Re: fleshofgods]
    #1501148 - 04/28/03 05:32 PM (13 years, 8 months ago)

yes, that ought to work. That's what I use.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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