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Offlinelionsman
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Registered: 08/02/09
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Re: V. bombycina grow [Re: BlueLightRain]
    #14714566 - 07/04/11 07:44 AM (12 years, 7 months ago)

I have listed some wedges on Bemushroomed. There's one left if anyone wants it. I have sent it to a spreader in the US who will make it available there, so would prefer if it's taken by another country.
Cheers

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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: V. bombycina grow [Re: lionsman]
    #14715393 - 07/04/11 12:01 PM (12 years, 7 months ago)

Hi lionsman, I got the tube but it was sealed and when I placed som on agar it exploded into a mound of trich and black pin mold.

Lipa

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Offlineraughter
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Registered: 02/01/10
Posts: 16
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Re: V. bombycina grow [Re: lipa]
    #14716352 - 07/04/11 03:40 PM (12 years, 7 months ago)

Damn! What bad news. I'm psyched about this mushroom.
Lionsman, are you down for another try?

What is the recommended protocol for transcontinental culture shipping?

~r


--------------------
-----------------------------
Looking for paddy straw, GIANT paddy straw,
V. bombacyna, Macrocybe crassa.

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InvisibleJavadog
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Re: V. bombycina grow [Re: lipa]
    #14717829 - 07/04/11 09:55 PM (12 years, 7 months ago)

Quote:

lipa said:
Hi lionsman, I got the tube but it was sealed and when I placed som on agar it exploded into a mound of trich and black pin mold.

Lipa




Serious bummer!

Thank you both for trying!

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Offlinelionsman
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Re: V. bombycina grow [Re: Javadog]
    #14719173 - 07/05/11 07:05 AM (12 years, 7 months ago)

OK, thats bad news Lipa. I'll send you some more, probably as wedges.

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Invisiblelipa

Registered: 07/24/07
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Re: V. bombycina grow [Re: lionsman]
    #14719663 - 07/05/11 09:38 AM (12 years, 7 months ago)

Thanks bro. Appreciate it!

Lipa

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Offlinehoudinihar
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Re: V. bombycina grow [Re: lipa]
    #14725759 - 07/06/11 12:52 PM (12 years, 7 months ago)

what temps are you running lionsman?
also, did you sterilize or merely pasteurize your successful substrate?

houdinihar

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Offlinelionsman
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Re: V. bombycina grow [Re: houdinihar]
    #14749790 - 07/11/11 06:02 AM (12 years, 7 months ago)

Houdinihar.
Temps were variable while these were growing. In the teens (C) growth was slow, but was rapid when it got warmer (25 C). I don't know whats optimum, but mid to late 20s C seems about right. All my substrates were sterilized. At some point it needs to be done on pasteurised straw, but I haven't had time yet.

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Offlinehoudinihar
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Re: V. bombycina grow [Re: lionsman]
    #14750225 - 07/11/11 08:48 AM (12 years, 7 months ago)

thanks lionsman.

houdinihar

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OfflineMycelio
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Re: V. bombycina grow [Re: houdinihar]
    #14833781 - 07/27/11 03:29 PM (12 years, 7 months ago)

The other day I had a jar, refusing to fruit for weeks. As it was already moldy and there was nothing to lose, I sprinkled some sterilized soil (old, sandy potting soil) on top, not enough to cover the surface completely, just to see what would happen. The mycelium vigorously grew through the soil and up into the air, collapsed, formed numerous knots and some turned into tiny eggs within a few days. So soil can definitely act as a catalyst for primordia formation, while microbes are not necessary.

Carsten






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InvisibleNot Quite Social
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Registered: 07/16/10
Posts: 1,418
Loc: Midwest
Re: V. bombycina grow [Re: Mycelio]
    #14833863 - 07/27/11 03:49 PM (12 years, 7 months ago)

Wow ...

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Invisiblelipa

Registered: 07/24/07
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Re: V. bombycina grow [Re: Mycelio]
    #14833992 - 07/27/11 04:23 PM (12 years, 7 months ago)

Looks like a spun cocoon.

Lipa

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Offlinehoudinihar
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Re: V. bombycina grow [Re: lipa]
    #14834455 - 07/27/11 05:59 PM (12 years, 7 months ago)

very nice pictures, Mycelio.
they DO look like little cocoons all wrapped in silk.

houdinihar

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InvisibleJavadog
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Re: V. bombycina grow [Re: houdinihar]
    #14835017 - 07/27/11 07:48 PM (12 years, 7 months ago)

Yes they do.  Very nice photos.  Thanks for sharing!

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Offlinevishal779
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Re: V. bombycina grow [Re: Javadog]
    #14836559 - 07/28/11 01:12 AM (12 years, 7 months ago)

looks very pritty ..would have been better if u inoculated that one to normal wet straw.. Good to see u finally succeeded with it in some way..
Vishal..

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OfflineMycelio
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Re: V. bombycina grow [Re: vishal779]
    #14837327 - 07/28/11 06:03 AM (12 years, 7 months ago)

Thanks guys! These eggs are really fascinating, I have to look at them every couple of hours.

Hi Vishal, this is wheat straw with 5% rice and a little bit of yeast extract, sterilized and inoculated with substrate spawn of the same kind. On straw alone this species only gives weak growth.
Did you ever grow V. bombycina? There may be differences to V. volvacea.

Carsten

Edited by Mycelio (07/28/11 06:04 AM)

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Offlinevishal779
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Re: V. bombycina grow [Re: Mycelio]
    #14837340 - 07/28/11 06:12 AM (12 years, 7 months ago)

no i have not yet grew any of those, but from the data i have read Vv, Vd(diplasia) and Vb (bombycina) can be grown on same substrates with same technic. Am about to get a culture from Lionsman in next week or so.
Vishal.

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OfflineMycelio
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Re: V. bombycina grow [Re: vishal779]
    #14837412 - 07/28/11 06:47 AM (12 years, 7 months ago)

Perhaps have a look here:
Experimental cultivation of the silver-silk straw mushroom Volvarielle bombicyna (Per.ex.Fr) Sing. isolated in Vietnam
Tran Thi Kim Dung and Pham Thanh Ho
http://tainguyenso.vnu.edu.vn/jspui/bitstream/123456789/9759/1/Experimental%20cultivation%20of%20the%20silver.pdf

As Lionsmans strain is different, it would be extremely interesting if you could compare V.b. and V.v. on the same substrate and under the same conditions.

I'll look into the usability of spent Pleurotus substrate.

Carsten

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InvisibleJavadog
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Re: V. bombycina grow [Re: Mycelio]
    #14837604 - 07/28/11 08:18 AM (12 years, 7 months ago)

Hey,

That is a very interesting paper.  Thanks for sharing!  I read there
that they used a "proportion of spawn/substrate is 2%."  1:50.  Wow.

I will try some Volvarielle some time.

Take care,

Javadog

P.S. I should have mentioned my appreciation of the no-microbes factoid.  I am
not sure that they would not help, but the fact that you got fruits without
them was not lost on me.


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineMycelio
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Re: V. bombycina grow [Re: Javadog]
    #14838150 - 07/28/11 11:01 AM (12 years, 7 months ago)

You're welcome! I'm always glad to spread some knowledge and pave the way for later experiments.

About the soil, I think it mainly helps with small amounts of substrate in less than optimum conditions. Otherwise this species should fruit fine by itself, just like V. volvacea.

The low spawn rate in that paper sounds plausible for straw with very little supplements and a long pasteurization time. On a closer look, there are some odd aspects regarding the amount of supplements. For rice bran, they say they tested 1, 5 and 10‰ (not %). Later they talk about best results with 2% bran. This raises suspicions, that they either mixed up numbers or units. So we better not fully trust in every word.

Carsten

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