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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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worth waiting, or just start over?
#14815178 - 07/23/11 08:20 PM (12 years, 8 months ago) |
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so ive made two large syringes from one print, procedure was very sterile except i only rinsed the jar which the spores were scraped into with water instead of putting in the pc. this was my only flaw, i dont know what i was thinking, ive inoculated jars. are they worth waiting for? or should i just empty them out and restart
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willgeezy
Registered: 01/14/10
Posts: 248
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Re: worth waiting, or just start over? [Re: DAHMER]
#14815245 - 07/23/11 08:31 PM (12 years, 8 months ago) |
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Use em they'll prolly work....if not just do more jars you should see some kinda growth in your jars within the week especially since you already inoculated
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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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Re: worth waiting, or just start over? [Re: willgeezy]
#14815260 - 07/23/11 08:35 PM (12 years, 8 months ago) |
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i just hope its not a rainbow instead of pure white, or even by chance, the first syringe took the punch and rinsed the jar with sterile water
Edited by DAHMER (07/23/11 08:48 PM)
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ModularMind
M.P.F.
Registered: 02/09/10
Posts: 7,902
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Re: worth waiting, or just start over? [Re: DAHMER]
#14816092 - 07/23/11 11:39 PM (12 years, 8 months ago) |
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Wipe it out with alcohol next time.
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UnnamedGrower
The AMUiest
Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
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Re: worth waiting, or just start over? [Re: ModularMind]
#14816099 - 07/23/11 11:40 PM (12 years, 8 months ago) |
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Quote:
ModularMind said: Wipe it out with alcohol next time.
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ozzysmygod
Late Night PC'ing Enthusiast
Registered: 11/26/08
Posts: 835
Last seen: 9 years, 5 months
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Re: worth waiting, or just start over? [Re: UnnamedGrower]
#14816593 - 07/24/11 04:41 AM (12 years, 8 months ago) |
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Pressure cook it next time, dont use alcohol it doesnt kill spores
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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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Re: worth waiting, or just start over? [Re: ozzysmygod]
#14816834 - 07/24/11 07:57 AM (12 years, 8 months ago) |
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ill do the pc, alcohol will create a bigger problem
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ModularMind
M.P.F.
Registered: 02/09/10
Posts: 7,902
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Re: worth waiting, or just start over? [Re: DAHMER]
#14817010 - 07/24/11 09:38 AM (12 years, 8 months ago) |
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Quote:
DAHMER said: alcohol will create a bigger problem
What problem?
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ozzysmygod
Late Night PC'ing Enthusiast
Registered: 11/26/08
Posts: 835
Last seen: 9 years, 5 months
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Re: worth waiting, or just start over? [Re: ModularMind]
#14817073 - 07/24/11 10:04 AM (12 years, 8 months ago) |
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It doesnt sterilize it sanitizes. It does not kill spores.
If he has a PC, he should sterilize his jar in a PC
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ModularMind
M.P.F.
Registered: 02/09/10
Posts: 7,902
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Re: worth waiting, or just start over? [Re: ozzysmygod]
#14817080 - 07/24/11 10:08 AM (12 years, 8 months ago) |
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Complete overkill for making a spore syringe IMO.
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k00laid
NEMO
Registered: 05/03/10
Posts: 19,636
Last seen: 8 months, 1 day
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Re: worth waiting, or just start over? [Re: ModularMind]
#14817296 - 07/24/11 11:21 AM (12 years, 8 months ago) |
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i thought everyone pc'd the shotglass they scraped their spores into.
as well as the tool used for scraping the spores.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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Re: worth waiting, or just start over? [Re: ModularMind]
#14817298 - 07/24/11 11:22 AM (12 years, 8 months ago) |
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alcohol could possibly add contaminants, unless it is the first pour from a brand new bottle, ill stick with the pc. but my question was whether or not you guys think i should wait for the jars or start over becouse of this flaw.
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UnnamedGrower
The AMUiest
Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
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Re: worth waiting, or just start over? [Re: DAHMER]
#14817301 - 07/24/11 11:23 AM (12 years, 8 months ago) |
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Id start on a new batch but I am always starting a new batch
I wouldnt be surprised if your syringes are good tho if you followed other sterile tek
Edited by UnnamedGrower (07/24/11 11:23 AM)
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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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Re: worth waiting, or just start over? [Re: k00laid]
#14817303 - 07/24/11 11:23 AM (12 years, 8 months ago) |
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Quote:
k00laid said: i thought everyone pc'd the shotglass they scraped their spores into.
as well as the tool used for scraping the spores.
i used a new scalpel, and a brand new jar, just forgot to pc it
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ModularMind
M.P.F.
Registered: 02/09/10
Posts: 7,902
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Re: worth waiting, or just start over? [Re: DAHMER]
#14817371 - 07/24/11 11:46 AM (12 years, 8 months ago) |
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Quote:
DAHMER said: alcohol could possibly add contaminants, unless it is the first pour from a brand new bottle
Thanks for the heads up. Do you alternatively suggest "rinsing the jar out with water instead"?
Quote:
DAHMER said:
Quote:
k00laid said: i thought everyone pc'd the shotglass they scraped their spores into.
as well as the tool used for scraping the spores.
i used a new scalpel, and a brand new jar, just forgot to pc it
I think k00laid is being somewhat sarcastic. I've heard tell of 0% failure with some techniques from people who don't even own a PC.
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ozzysmygod
Late Night PC'ing Enthusiast
Registered: 11/26/08
Posts: 835
Last seen: 9 years, 5 months
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Re: worth waiting, or just start over? [Re: ModularMind]
#14817391 - 07/24/11 11:51 AM (12 years, 8 months ago) |
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Water could add as many spores as alcohol...
PC it
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ModularMind
M.P.F.
Registered: 02/09/10
Posts: 7,902
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Re: worth waiting, or just start over? [Re: ozzysmygod]
#14817409 - 07/24/11 11:55 AM (12 years, 8 months ago) |
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Why not just boil it in a pot? That should be sufficient. That's also overkill IMO.
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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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Re: worth waiting, or just start over? [Re: ModularMind]
#14817430 - 07/24/11 11:58 AM (12 years, 8 months ago) |
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i used to boil or steam it, but now i pc. im just hoping that i didnt screw up my batch of jars. but most likely i did. ill see if any are contam free, then toss the rest. thanks for your time everyone
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FuckMeRunnin
REALLY good looking
Registered: 07/18/09
Posts: 788
Last seen: 10 years, 10 months
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Re: worth waiting, or just start over? [Re: DAHMER]
#14817473 - 07/24/11 12:07 PM (12 years, 8 months ago) |
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When I make spore prints, I just clean out the jars with soap and water(some times just water), and I have yet to have a problem. Pcing a jar for a spore print is sooo much overkill it's not even funny. If I have alcohol handy, like within arms reach ill swoosh some of that around in the jar. But yeah, just do your thing your fine.
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DAHMER
eye of storm
Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
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Re: worth waiting, or just start over? [Re: FuckMeRunnin]
#14817561 - 07/24/11 12:22 PM (12 years, 8 months ago) |
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Quote:
FuckMeRunnin said: When I make spore prints, I just clean out the jars with soap and water(some times just water), and I have yet to have a problem. Pcing a jar for a spore print is sooo much overkill it's not even funny. If I have alcohol handy, like within arms reach ill swoosh some of that around in the jar. But yeah, just do your thing your fine.
i didnt make a print, i made two syringes with a print, 90cc total
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