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OfflineDAHMER
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Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
worth waiting, or just start over?
    #14815178 - 07/23/11 08:20 PM (12 years, 8 months ago)

so ive made two large syringes from one print, procedure was very sterile except i only rinsed the jar which the spores were scraped into with water instead of putting in the pc. this was my only flaw, i dont know what i was thinking, ive inoculated jars. are they worth waiting for? or should i just empty them out and restart

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Invisiblewillgeezy
Registered: 01/14/10
Posts: 248
Re: worth waiting, or just start over? [Re: DAHMER]
    #14815245 - 07/23/11 08:31 PM (12 years, 8 months ago)

Use em they'll prolly work....if not just do more jars you should see some kinda growth in your jars within the week especially since you already inoculated

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OfflineDAHMER
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Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
Re: worth waiting, or just start over? [Re: willgeezy]
    #14815260 - 07/23/11 08:35 PM (12 years, 8 months ago)

i just hope its not a rainbow instead of pure white, or even by chance, the first syringe took the punch and rinsed the jar with sterile water:rolleyes:

Edited by DAHMER (07/23/11 08:48 PM)

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InvisibleModularMind
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Registered: 02/09/10
Posts: 7,902
Re: worth waiting, or just start over? [Re: DAHMER]
    #14816092 - 07/23/11 11:39 PM (12 years, 8 months ago)

Wipe it out with alcohol next time.

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InvisibleUnnamedGrower
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Registered: 04/17/11
Posts: 7,146
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Re: worth waiting, or just start over? [Re: ModularMind]
    #14816099 - 07/23/11 11:40 PM (12 years, 8 months ago)

Quote:

ModularMind said:
Wipe it out with alcohol next time.



:whathesaid:

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Offlineozzysmygod
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Registered: 11/26/08
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Re: worth waiting, or just start over? [Re: UnnamedGrower]
    #14816593 - 07/24/11 04:41 AM (12 years, 8 months ago)

Pressure cook it next time, dont use alcohol it doesnt kill spores

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OfflineDAHMER
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Registered: 02/24/11
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Re: worth waiting, or just start over? [Re: ozzysmygod]
    #14816834 - 07/24/11 07:57 AM (12 years, 8 months ago)

ill do the pc, alcohol will create a bigger problem

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InvisibleModularMind
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Re: worth waiting, or just start over? [Re: DAHMER]
    #14817010 - 07/24/11 09:38 AM (12 years, 8 months ago)

Quote:

DAHMER said:
alcohol will create a bigger problem




What problem?

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Offlineozzysmygod
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Re: worth waiting, or just start over? [Re: ModularMind]
    #14817073 - 07/24/11 10:04 AM (12 years, 8 months ago)

It doesnt sterilize it sanitizes. It does not kill spores.

If he has a PC, he should sterilize his jar in a PC

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InvisibleModularMind
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Re: worth waiting, or just start over? [Re: ozzysmygod]
    #14817080 - 07/24/11 10:08 AM (12 years, 8 months ago)

Complete overkill for making a spore syringe IMO.

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Offlinek00laid
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Re: worth waiting, or just start over? [Re: ModularMind]
    #14817296 - 07/24/11 11:21 AM (12 years, 8 months ago)

i thought everyone pc'd the shotglass they scraped their spores into.

as well as the tool used for scraping the spores.


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OfflineDAHMER
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Registered: 02/24/11
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Re: worth waiting, or just start over? [Re: ModularMind]
    #14817298 - 07/24/11 11:22 AM (12 years, 8 months ago)

alcohol could possibly add contaminants, unless it is the first pour from a brand new bottle, ill stick with the pc. but my question was whether or not you guys think i should wait for the jars or start over becouse of this flaw.:syringe:

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InvisibleUnnamedGrower
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Re: worth waiting, or just start over? [Re: DAHMER]
    #14817301 - 07/24/11 11:23 AM (12 years, 8 months ago)

Id start on a new batch but I am always starting a new batch:shrug2:

I wouldnt be surprised if your syringes are good tho if you followed other sterile tek

Edited by UnnamedGrower (07/24/11 11:23 AM)

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OfflineDAHMER
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Registered: 02/24/11
Posts: 90
Loc: australia
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Re: worth waiting, or just start over? [Re: k00laid]
    #14817303 - 07/24/11 11:23 AM (12 years, 8 months ago)

Quote:

k00laid said:
i thought everyone pc'd the shotglass they scraped their spores into.

as well as the tool used for scraping the spores.




i used a new scalpel, and a brand new jar, just forgot to pc it

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InvisibleModularMind
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Re: worth waiting, or just start over? [Re: DAHMER]
    #14817371 - 07/24/11 11:46 AM (12 years, 8 months ago)

Quote:

DAHMER said:
alcohol could possibly add contaminants, unless it is the first pour from a brand new bottle




Thanks for the heads up.
Do you alternatively suggest "rinsing the jar out with water instead"?
Quote:

DAHMER said:
Quote:

k00laid said:
i thought everyone pc'd the shotglass they scraped their spores into.

as well as the tool used for scraping the spores.




i used a new scalpel, and a brand new jar, just forgot to pc it




I think k00laid is being somewhat sarcastic.
I've heard tell of 0% failure with some techniques from people who don't even own a PC.

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Offlineozzysmygod
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Re: worth waiting, or just start over? [Re: ModularMind]
    #14817391 - 07/24/11 11:51 AM (12 years, 8 months ago)

Water could add as many spores as alcohol...

PC it

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InvisibleModularMind
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Re: worth waiting, or just start over? [Re: ozzysmygod]
    #14817409 - 07/24/11 11:55 AM (12 years, 8 months ago)

Why not just boil it in a pot?
That should be sufficient.
That's also overkill IMO.

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OfflineDAHMER
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Registered: 02/24/11
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Re: worth waiting, or just start over? [Re: ModularMind]
    #14817430 - 07/24/11 11:58 AM (12 years, 8 months ago)

i used to boil or steam it, but now i pc. im just hoping that i didnt screw up my batch of jars. but most likely i did. ill see if any are contam free, then toss the rest. thanks for your time everyone

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OfflineFuckMeRunnin
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Re: worth waiting, or just start over? [Re: DAHMER]
    #14817473 - 07/24/11 12:07 PM (12 years, 8 months ago)

When I make spore prints, I just clean out the jars with soap and water(some times just water), and I have yet to have a problem. Pcing a jar for a spore print is sooo much overkill it's not even funny. If I have alcohol handy, like within arms reach ill swoosh some of that around in the jar. But yeah, just do your thing your fine.


--------------------
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OfflineDAHMER
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Registered: 02/24/11
Posts: 90
Loc: australia
Last seen: 12 years, 6 months
Re: worth waiting, or just start over? [Re: FuckMeRunnin]
    #14817561 - 07/24/11 12:22 PM (12 years, 8 months ago)

Quote:

FuckMeRunnin said:
When I make spore prints, I just clean out the jars with soap and water(some times just water), and I have yet to have a problem. Pcing a jar for a spore print is sooo much overkill it's not even funny. If I have alcohol handy, like within arms reach ill swoosh some of that around in the jar. But yeah, just do your thing your fine.


i didnt make a print, i made two syringes with a print, 90cc total

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