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OfflineProfessorPinHead
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No-Pour Agar Agar TEK * 11
    #14788118 - 07/18/11 07:53 PM (9 years, 9 months ago)

I know a lot of you guys might not have a flowhood so I figured I would let the secret out on how I used to skip the pouring step when making agar plates.

Working in a glove box can be a pain and the less you expose your cultures to the still air in them the better.

------


Materials needed:

Broth:

- 10 grams Agar Agar powder or flakes.
(Note: Some of the food grade Agar on the market has preservatives in it, the best results will come from a quality preservative free Agar, examples of good brands are NOW, Tic Gums, or Fungi Perfecti Agar)


- 10 grams light malt extract.



-500 ml H2O.




Plates:

250 ml (8oz) Ball 'elite' Mason canning Jars.



Wide mouth synthetic  filter discs.

(If you don't have filter discs you can use Citric's self heal lids and they work just as well, Just make sure to drill a third hole to prevent overboiling in the PC )


Wide mouth canning jars lids with (2) 5/16 inch holes drilled in each one.
This keeps the agar from over boiling while it is being pressure cooked.


Wide mouth rings.



Cooking the Broth:





Put 500 ml COLD H2O in a clean pot



Add 10 grams light malt extract.

and 10 grams Agar Agar flakes.


You are going to want to start off with cold water and add the ingredients, then bring the media to a slow simmer, the broth should look slightly chunky at first but it will melt after a few minutes of stirring.



Simmer at a low boil for approx. 3-5 minutes or
until the majority of the flakes have melted.



NOW CAREFULLY POUR APPROX. 60ML OF YOUR AGAR BROTH INTO EACH JAR





Assembling the Plates:



To assemble your plate first you lay the lid down bottom side faced up on your jar
.


Next you lay the filter disc on top of the lid.


Screw on the ring firmly, but not too tight.


Cover your jars with foil just like when you make grain or PF jars.

Stack your jars in your PC with hot tap water just barely coming above the liner insert,
about 5mm above the bottom of the jar.


Finally, PC for 45 minutes at 15 PSI and let the Jars cool & set over night.

The following day after letting them cool overnight in the PC you can inoculate them in a glovebox or in front of a laminar flowhood.

This is  what your final product should look like after you add
your favorite mycelium!




Edited by ProfessorPinHead (08/31/15 08:44 AM)


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InvisibleShadOWCrack
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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #14788348 - 07/18/11 08:41 PM (9 years, 9 months ago)

nice bro!!
I need to get some parafilm lol.
the dishes I ordered this time will need it lol


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InvisibleCarl Sagan
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Re: Prof's No-Pour Agar Agar TEk. [Re: ShadOWCrack] * 1
    #14788444 - 07/18/11 08:58 PM (9 years, 9 months ago)



That is a beautiful mono.

Shadow; try this


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InvisibleEvilMushroom666
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Re: Prof's No-Pour Agar Agar TEk. [Re: Carl Sagan] * 1
    #14788610 - 07/18/11 09:29 PM (9 years, 9 months ago)

:borat:


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InvisibleShadOWCrack
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Re: Prof's No-Pour Agar Agar TEk. [Re: Carl Sagan] * 1
    #14788759 - 07/18/11 09:55 PM (9 years, 9 months ago)

Quote:

Carl Sagan said:


That is a beautiful mono.

Shadow; try this



lol


this was my first clone


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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #14788772 - 07/18/11 09:58 PM (9 years, 9 months ago)

Quote:

ProfessorPinHead said:
the secret




Was that what this was?

:ilold:

Well written, good pics, not too fancy, but good use of fonts and color.

:highfive:


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OfflineOoBYCoO
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Re: Prof's No-Pour Agar Agar TEk. [Re: Doc_T] * 1
    #14788884 - 07/18/11 10:15 PM (9 years, 9 months ago)

:awedance:  Love it!


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Invisiblepenhed
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Re: Prof's No-Pour Agar Agar TEk. [Re: OoBYCoO] * 1
    #14789044 - 07/18/11 10:41 PM (9 years, 9 months ago)

and this method has served me well.........great share PP:bigyesnod:


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OfflineProfessorPinHead
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Re: Prof's No-Pour Agar Agar TEk. [Re: penhed] * 1
    #14789164 - 07/18/11 11:01 PM (9 years, 9 months ago)

Gracias muchachos!


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OfflineProfessorPinHead
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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #14901510 - 08/10/11 03:49 PM (9 years, 8 months ago)

This is what it looks like if you use a piece of a spawn bag with the jar instead of using one of the filter discs.
[image][/image]


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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #14901529 - 08/10/11 03:53 PM (9 years, 8 months ago)

:headbang3:


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OfflineCAP_TURTLE
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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #16649038 - 08/05/12 08:31 PM (8 years, 8 months ago)

Couple questions since I'm about to do something very similar to this tek. First instead of using filter discs I used plastic lids and melted 3 1/4" holes across the lid. I made the middle one into a silicone injection port and placed micropore over the other two. I'm wondering if this will suffice for fae and prevent any boil over or any other reason my idea won't work. Also a lot of ppl preach by fast Fred's and your recipe is slightly different so I'm wondering if you've experimented and found yours better or if I should follow fast freds my first time. Also since I'm unable to measure each one precisely how thick would u say I should aim for in the middle of the jar?


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OfflineProfessorPinHead
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Re: Prof's No-Pour Agar Agar TEk. [Re: CAP_TURTLE] * 1
    #17080197 - 10/22/12 06:28 PM (8 years, 6 months ago)

I just received a pm from a member informing me all the picture links in this tek were broken,

There was a little bit of updating that needed to be done also so I edited the pix and fixed them and added the extra info.

Hopefully the tek is more useful to people with the pictures added, :facepalm:


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Invisibletoxetel
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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #17123075 - 10/29/12 12:49 PM (8 years, 6 months ago)

Thanks for the writeup, Professor! Where do you find the 125ml widemouth jars? I can't seem to find them anywhere in person or online.


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InvisibleVaeVictum
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Re: Prof's No-Pour Agar Agar TEk. [Re: toxetel] * 1
    #17123084 - 10/29/12 12:52 PM (8 years, 6 months ago)

Ty for taking the time to write this up and take the pictures. If there's one thing M.Cult needs more of it's definitely detailed Tek's like this one. :thumbup:

Kudos.

:kenthumbup:


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OfflineProfessorPinHead
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Re: Prof's No-Pour Agar Agar TEk. [Re: VaeVictum] * 1
    #17124110 - 10/29/12 03:33 PM (8 years, 6 months ago)

Quote:

toxetel said:
Thanks for the writeup, Professor! Where do you find the 125ml widemouth jars? I can't seem to find them anywhere in person or online.



You need to punch in the words 'elite' ball mason jars when searching for them.
You will want the 8oz ones for Agar work. I actually use the 16 ounce ones for exotic side projects like Zappos, etc....

Here is a link to get you started, :smile:


Quote:

VaeVictum said:
Ty for taking the time to write this up and take the pictures. If there's one thing M.Cult needs more of it's definitely detailed Tek's like this one. :thumbup:

Kudos.

:kenthumbup:



No problem man, :hatsoff:


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Invisibletoxetel
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Re: Prof's No-Pour Agar Agar TEk. [Re: ProfessorPinHead] * 1
    #17126397 - 10/29/12 09:04 PM (8 years, 6 months ago)

Awesome! Thanks so much! It's so useful to have specifics with a tek.


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OfflineTrippy_Smurf
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Re: Prof's No-Pour Agar Agar TEk. [Re: toxetel] * 1
    #17246136 - 11/19/12 02:09 PM (8 years, 5 months ago)

How many jars will one batch make?


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Invisibleserotonin storm
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Re: Prof's No-Pour Agar Agar TEk. [Re: Trippy_Smurf] * 1
    #17246272 - 11/19/12 02:39 PM (8 years, 5 months ago)

I used to use these ball elite jars, but they have a convex bottom, but I can pour ten flat bottom Tupperware containers with 500ml of agar media, they have identical diameters. How many you get really depends on how much solution you pour in and how big the jar/container/petri dish


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OfflineProfessorPinHead
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Re: Prof's No-Pour Agar Agar TEk. [Re: Trippy_Smurf] * 1
    #17246768 - 11/19/12 04:11 PM (8 years, 5 months ago)

Quote:

Trippy_Smurf said:
How many jars will one batch make?



That depends on how much agar you make and how many jars you have.

If you follow the measurements in the op you should be able to get 8 no problem out of 500ml agar. You may even be able to stretch it to 10 if you pour a little thin.


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