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OfflineFungal-one
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Time to learn a new skill!
    #14778019 - 07/16/11 05:50 PM (9 years, 9 months ago)

I'm finally ready to start the spores I took from some GT I had like 1.5-2 years ago. They are just wrapped up in foil and no doubt contaminated. I bought a huge bag of sterile petri dishes and ready to pour agar to get them started. Seems like I remember reading about using dirty spores a while back, and how on agar in a petri was the preferred method since it allowed you to then use a sterile exacto knife and cut out healthy desirable/fungal growth which could then be spawned on a larger scale. I'm not holding a ton of hope for them. If it works, great, I get to learn a new skill and I'll have what I already know to be some pretty active lil' knot heads. On the other hand, I'm already planning on ordering a few fresh syringes of APE soon anyway. So.....

Wish a kneegrow luck!


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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OfflineFungal-one
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14823242 - 07/25/11 06:22 PM (9 years, 9 months ago)

It begins


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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OfflineChez man
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14823434 - 07/25/11 07:14 PM (9 years, 9 months ago)

You got a lot of spores going into each dish.....you only need a few specks....


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http://www.hedweb.com/hedab.htm


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InvisibleHorizonSpawn
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Re: Time to learn a new skill! [Re: Chez man]
    #14823506 - 07/25/11 07:31 PM (9 years, 9 months ago)

The fewer spores per petri dish, the better... Well; at least two health ones, lol...


From what I've studied; petri work is THE way it's done :smile: You should be fine. This is how wild specimens are "cleaned up", too (spore and tissue, alike)  :smile:


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Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:


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OfflineFungal-one
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Re: Time to learn a new skill! [Re: Chez man]
    #14823512 - 07/25/11 07:32 PM (9 years, 9 months ago)

Yeah, it's my first time :lol:

It's been like an hour, I wonder if I have anything I can pick yet?


--------------------
Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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Invisibleandymc
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14823664 - 07/25/11 07:58 PM (9 years, 9 months ago)

You're using a glove box, I hope?  :tongue:  When I see what looks like a spore print exposed to the open air, I get sad.


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How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflineFungal-one
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Re: Time to learn a new skill! [Re: andymc]
    #14823686 - 07/25/11 08:01 PM (9 years, 9 months ago)

You've obviously never seen the hilarious video of me taking those prints. It was more like, I'm going to eat these, but I should save some of spores for the hell of it. I was not trying to be all sterile and whatnot. So, these have been contaminated from the get go.

:lolsy:

This is so funny to me. As you can hear I talk a lot of shit to the shroomery about your fears of contamination and 'tainted' prints ;lol:



--------------------
Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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Invisibleandymc
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14823737 - 07/25/11 08:09 PM (9 years, 9 months ago)

:laugh2:

Oh god, I got about 2 minutes in and had to stop watching.

Yeah, you're right, I wouldn't worry about trying to keep that print clean!  :lmafo:

Yes, agar is definitely the way to go here.  But, I hope you've made a glove box for the agar work.  It's pretty unforgiving if your clean techniques aren't followed carefully.  The problem is that competitors like bacteria, trich, etc. grow faster than those spores will germinate.  Give your spores the best possible chance to get established without contams, or else it will be a long, tough battle to get a clean mycelium sample.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflineFungal-one
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Re: Time to learn a new skill! [Re: andymc]
    #14823759 - 07/25/11 08:12 PM (9 years, 9 months ago)

I'm good at making glove boxes lol. I have a natural talent for that, just not for taking clean prints.


--------------------
Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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OfflineWing
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14823816 - 07/25/11 08:24 PM (9 years, 9 months ago)

I love how one gets dropped!


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My Old Grow Logs



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Invisibleandymc
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Re: Time to learn a new skill! [Re: Wing]
    #14824032 - 07/25/11 09:03 PM (9 years, 9 months ago)

Remember it can be a good few days before you see any activity from the spores.  I spread some spores on agar at least a week ago, and it was only yesterday I saw the first growth (but they're going fast now)


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflineFungal-one
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Re: Time to learn a new skill! [Re: andymc]
    #14824059 - 07/25/11 09:07 PM (9 years, 9 months ago)

I'm thinking about buying a microscope for this project.


--------------------
Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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Invisibleandymc
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14824072 - 07/25/11 09:11 PM (9 years, 9 months ago)

Quote:

Fungal-one said:
I'm thinking about buying a microscope for this project.




I often wish I had one.  A good microscope would add a new dimension to this hobby.

Unfortunately I spend too much dough building extravagantly expensive flow hoods and stuff like that.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflineFungal-one
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Registered: 07/14/10
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Re: Time to learn a new skill! [Re: andymc]
    #14824325 - 07/25/11 09:54 PM (9 years, 9 months ago)

I saw a pretty nice digital one with usb for $150 at the science store the other day. That's not sooo bad.


--------------------
Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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InvisibleHorizonSpawn
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Registered: 05/14/11
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14824805 - 07/25/11 11:38 PM (9 years, 9 months ago)

You want a minimum of 400 x magnification for Mycology :smile:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:


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OfflineFungal-one
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Re: Time to learn a new skill! [Re: HorizonSpawn]
    #14850738 - 07/31/11 10:56 AM (9 years, 9 months ago)

Quote:

HorizonSpawn said:
You want a minimum of 400 x magnification for Mycology :smile:



Are you serious about 400x or are just encouraging me to buy badass toys? I had a closer look at the one for $150 and it's only like 40x or some shit. Realistically, how strong a scope do I need for a hobby involving fungus?

Here's my plates this morning. It's hilarious how many spores I put on there. Oh well, now I know. I have no shame in doing things fucked up the first couple times lol.


--------------------
Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.


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Offlinek00laid
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Re: Time to learn a new skill! [Re: Fungal-one]
    #14850916 - 07/31/11 11:50 AM (9 years, 9 months ago)

Quote:

Fungal-one said:
It's hilarious how many spores I put on there.



:lolsy:

i enjoyed the pics, thanks for postin dawg


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AMU - AMU Q & A - MyVideo Teks!


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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Bridgetown Botanicals CBD Edibles   PhytoExtractum Buy Bali Kratom Powder, Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Kratom Capsules for Sale   Amazon Agar, Laminar Flow Hood, Microscope, Petri Dish, Scales

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