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Offlineihavefingers
Stranger

Registered: 03/28/03 Happy 21st Shroomiversary!
Posts: 216
Last seen: 19 years, 9 months
air exchange
    #1476148 - 04/20/03 10:01 AM (20 years, 11 months ago)

when jars are incubating do they need air exchange?
i have three jars incubating in a sealed aquarium temp is at 84F, humidity is at 64%
the jars have the lids on them with the 4 holes from being innoculated and i put tin foil on top of that. If they need air exchange what would you recommend to do that besides fanning out the aquarium every so often should i take the foil off or the lid or anything?

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: air exchange [Re: ihavefingers]
    #1476156 - 04/20/03 10:06 AM (20 years, 11 months ago)

You can slightly crack the lids to jars open and invert the jars this will allow some built up c02 escape during the colonization process . Keeping jars in humid climate it kinda worthless at this point , so is fanning . Temp is the only important factor to concern yourself with .
Did not see holes in lid thing , just cover those holes with some micropore tape and invert , you are good to go , have patience and do not handle jars constantly .
Good Luck]
"May The Mushroom Goddess Bless All Your Endeavors !"
Trip n D


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: air exchange [Re: ihavefingers]
    #1476158 - 04/20/03 10:06 AM (20 years, 11 months ago)

Quote:

when jars are incubating do they need air exchange?



Yes.

Quote:

humidity is at 64%



Not a factor during incubation.

Quote:

If they need air exchange what would you recommend to do



Remove tape from holes (if taped), loosen lid slightly, loosen or remove foil.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Offlineihavefingers
Stranger

Registered: 03/28/03 Happy 21st Shroomiversary!
Posts: 216
Last seen: 19 years, 9 months
Re: air exchange [Re: dog]
    #1476178 - 04/20/03 10:14 AM (20 years, 11 months ago)

thank you for the input

if there isn't good airflow could the cause colonization to slow?
my jars have been incubating for almost two weeks... two of them are coming along rather nicely, one is really far behind but i have patience for these things. I'm excited for a final product of course, but you know it's worth the wait!

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: air exchange [Re: ihavefingers]
    #1476182 - 04/20/03 10:16 AM (20 years, 11 months ago)

There is no need to fan, if that's what you mean.  :smile: 


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Offlinetastyshroom
Mr.Shroom
Male

Registered: 03/15/03
Posts: 821
Loc: Washington
Last seen: 14 years, 11 months
Re: air exchange [Re: dog]
    #1476197 - 04/20/03 10:24 AM (20 years, 11 months ago)

4:20!!!!!!
IM SO HIGH!


--------------------
Perspective on life always changes

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: air exchange [Re: ihavefingers]
    #1476201 - 04/20/03 10:25 AM (20 years, 11 months ago)

Yes not allowing some air exchange (letting c02 escape) will slow growth in the later stages especially .
So use some micropore tape , or loosen the lids , and invert . C02 settles to bottom , and slowly escapes thru pores in tape (micropore tape by 3M ), or the slightly vented lid .
The slow ones may be shy !
What method of inoc did you use ? Syringe ?


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Offlineihavefingers
Stranger

Registered: 03/28/03 Happy 21st Shroomiversary!
Posts: 216
Last seen: 19 years, 9 months
Re: air exchange [Re: tripndicular]
    #1476350 - 04/20/03 11:28 AM (20 years, 11 months ago)

yes... i used syringe. Actually the one that is going slowly i reinnoculated four or five days ago and the growth has doubled but it's still behind. I posted that i did it and some people here advised against reinnoculating but the jar is fine. no contaminates and twice as much growth as it had so hey, i'm happy.




by the way... whenever i say "I" I actually mean "YOU"
in the paragraph above atleast.

and "I'M" means "YOU'RE"

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OfflineFatPed
Stranger
Registered: 07/09/06
Posts: 3
Last seen: 17 years, 6 months
Re: air exchange [Re: ihavefingers]
    #5838496 - 07/09/06 11:32 AM (17 years, 8 months ago)

Hello all first time grower.
Ive got 2 pf jars (10% growth) and they are completely sealed off, 2 layers of foil and a lid screwed on.

Is this ok or do i have to do something and change this?

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: air exchange [Re: FatPed]
    #5838538 - 07/09/06 11:44 AM (17 years, 8 months ago)

you should remove the foil if you have a dry verm layer.

and also any tape that might be covering the inoculation holes.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


Edited by Roadkill (07/09/06 11:46 AM)

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OfflineFatPed
Stranger
Registered: 07/09/06
Posts: 3
Last seen: 17 years, 6 months
Re: air exchange [Re: Roadkill]
    #5838563 - 07/09/06 11:56 AM (17 years, 8 months ago)

I do have a dry layer but its not overly deep, maybe half an inch?

(wow what a nice array of smilies Shroomery has provided :thumbup: :tongue2: :mushroom2:)

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: air exchange [Re: FatPed]
    #5838566 - 07/09/06 11:59 AM (17 years, 8 months ago)

thats more than enough...

when you pull the alum foil and tape away from the holes it will allow better gas exchange and the jars will take off faster.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Invisiblefastfred
Old Hand
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Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: air exchange [Re: Roadkill]
    #5838858 - 07/09/06 01:27 PM (17 years, 8 months ago)

The foil isn't air tight. It won't block gas exchange. I highly recommend leaving it on. If you are concerned just loosen it up a little bit. You should always have some sort of cover over your containers.

If you really think that verm layer is going to save you try this... Take a jar of rocks and drop a little sand in the jar and see how much makes it to the bottom. Then play with the jar like you would a PF jar and see how much sand is on the bottom. Contams are much smaller than grains of sand if the rocks were the size of verm bits. The rocks will stop some of the sand from getting to the bottom, but not all of it.


-FF

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: air exchange [Re: fastfred]
    #5838912 - 07/09/06 01:49 PM (17 years, 8 months ago)

I have been doing it this way for years....

and I never get a contam from leaving the inoculation holes uncovered.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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