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OfflineBigbus
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Agar's GLC method problems!
    #14758323 - 07/12/11 05:53 PM (13 years, 6 months ago)

Long time lurker, first time poster.

I've had many successes and very few failures in my journey of cultivation.  I've mastered pf teks and recently, rye has been a favorite.

I knocked up a rye jar from MS of GT and at colonization, I tried to make a GLC according to Agar's tek.  I got most of the mycelium water out and it had a kind of yellowish tint to it.

I used the syringes to knock up 24 more rye jars like an hour later.  It's been about 1.5 weeks and literally I have seen 0 growth.  Looks like the mycelium is growing ok inside the syringes inside my fridge.  Can anybody tell me what the problem could be? I've heard that this mycelium water starts grown within days.

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InvisibleeLShaMukO
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14758334 - 07/12/11 05:55 PM (13 years, 6 months ago)

provably bacteria.


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Offlinesage1o1
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Re: Agar's GLC method problems! [Re: eLShaMukO]
    #14758358 - 07/12/11 05:59 PM (13 years, 6 months ago)

I've run into that issue recently.  I just made a new jar of GLC.  Which can suck if you don't have any first or second gens left..


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Invisiblesandman420
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Re: Agar's GLC method problems! [Re: sage1o1]
    #14758375 - 07/12/11 06:01 PM (13 years, 6 months ago)

no growth is likely bacteria or yeast. This can be verified by opening a jar and giving it a whiff.


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Edited by sandman420 (07/12/11 06:01 PM)

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Invisiblesandman420
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Re: Agar's GLC method problems! [Re: sandman420]
    #14758383 - 07/12/11 06:02 PM (13 years, 6 months ago)

666 posts!?!?! Aghhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh! Gotta nip that in the bud.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineBigbus
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Re: Agar's GLC method problems! [Re: sandman420]
    #14758429 - 07/12/11 06:11 PM (13 years, 6 months ago)

So even though there's no visible bacteria and the rye is dry, contams in bacteria form are preventing mycelium growth?

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Offlinesage1o1
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14758461 - 07/12/11 06:17 PM (13 years, 6 months ago)

Everyone else might be right, but it may be due to you not getting any mycelium in the jars you were knocking up.  I could be wrong.


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"For me there is only the traveling on paths that have heart, on any path that may have heart, and the only worthwhile challenge is to traverse its full length--and there I travel looking, looking breathlessly." -Carlos Castenda  (Don Juan: the Sorcerer)

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Re: Agar's GLC method problems! [Re: sage1o1]
    #14758471 - 07/12/11 06:19 PM (13 years, 6 months ago)

Possible but rare, it's hard to get no mycelium at all in any jar.
But totally possible, and something worth checking.


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OfflineSlayer1024
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14758473 - 07/12/11 06:19 PM (13 years, 6 months ago)

You did let the rye completely colonize first right? your first post makes it sound as though you tried to make a GLC when you saw your first spot of myc. when I make a GLC its always clear with bits of myc floating around , no yellow


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OfflineBigbus
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Re: Agar's GLC method problems! [Re: Slayer1024]
    #14758535 - 07/12/11 06:29 PM (13 years, 6 months ago)

The GLC was fully colonized, almost solid like a cake, was a bitch to break up

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OfflineBigbus
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14758546 - 07/12/11 06:30 PM (13 years, 6 months ago)

I had 60 cc syringes, to be honest not sure if I could see the mycelium in the syringes, but after being in the fridge for a week, I see clumps of it growing

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Re: Agar's GLC method problems! [Re: Bigbus]
    #14758598 - 07/12/11 06:40 PM (13 years, 6 months ago)

Quote:

Bigbus said:
solid like a cake, was a bitch to break up




Bacteria.
Test a bit on some agar if you like.
Or shoot some into a regular karo or honey LC, watch it turn cloudy in a couple days.


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OfflineBigbus
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Re: Agar's GLC method problems! [Re: Doc_T]
    #14758633 - 07/12/11 06:45 PM (13 years, 6 months ago)

The master GLC jar had bacteria?  The master GLC jar was in a batch with 12 other jars which are colonizing a coir mix quite nicely right now though.

Edited by Bigbus (07/12/11 06:48 PM)

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OfflineBigbus
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14758719 - 07/12/11 07:01 PM (13 years, 6 months ago)

Assuming the jars are contam'd with bacteria, can I re-pc them and try knocking up again?

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Offlinedmonkey1
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14759139 - 07/12/11 08:12 PM (13 years, 6 months ago)

rule of thumb is to never re-pc and to start over


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OfflineBigbus
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Re: Agar's GLC method problems! [Re: dmonkey1]
    #14759243 - 07/12/11 08:33 PM (13 years, 6 months ago)

I'm gonna try to knock up a small jar of rye with the glc and see what happens... 24 jars of rye hurts balls :mad:

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Offlinesage1o1
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14759419 - 07/12/11 09:12 PM (13 years, 6 months ago)

Here check this place out, they're the cheapest I've seen for rye berries. 
www.nutsaregood.com

While the loss of 24 jars would suck for me, with that site in mind, it's really just the time that it took to make 'em not the cost of 24 cups of rye.  And the environment won't mind to get back some of its organic material, especially if you compost.


--------------------
An optimally setup greenhouse: (it's my GH :heart:)

Check it out if you have GH questions!

 

"For me there is only the traveling on paths that have heart, on any path that may have heart, and the only worthwhile challenge is to traverse its full length--and there I travel looking, looking breathlessly." -Carlos Castenda  (Don Juan: the Sorcerer)

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OfflineBigbus
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Re: Agar's GLC method problems! [Re: sage1o1]
    #14759447 - 07/12/11 09:18 PM (13 years, 6 months ago)

Na it wasn't the price, just the time, my local co-op has berries for like 99 cents a lb.

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Invisiblesandman420
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Re: Agar's GLC method problems! [Re: Bigbus]
    #14759645 - 07/12/11 09:56 PM (13 years, 6 months ago)

dont let it colonize so hard


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineBigbus
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Re: Agar's GLC method problems! [Re: sandman420]
    #14759901 - 07/12/11 10:38 PM (13 years, 6 months ago)

So I should just shake and use at like 90% ?

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Re: Agar's GLC method problems! [Re: Bigbus]
    #14759911 - 07/12/11 10:39 PM (13 years, 6 months ago)

Quote:

Bigbus said:
So I should just shake and use at like 90% ?





90%!?!?!?!

this is where you fucked up.


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Offlinescatmanrav
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Re: Agar's GLC method problems! [Re: dmonkey1]
    #14760231 - 07/12/11 11:36 PM (13 years, 6 months ago)

Quote:

dmonkey1 said:
Quote:

Bigbus said:
So I should just shake and use at like 90% ?





90%!?!?!?!

this is where you fucked up.




?? He didnt do it at 90%

Hardness had nothing to do with it. I bet you didnt give the mycelium time to recover in the water. Id try it again.

I have used jars over again, sometimes it works, sometimes not. I'd do a fresh one to make sure it works, if it does, try them again. If nothing, make new ones.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
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OfflineBigbus
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Re: Agar's GLC method problems! [Re: scatmanrav]
    #14760980 - 07/13/11 05:37 AM (13 years, 6 months ago)

Hmm... so the mycelium has to recover in the h2o before I use it? Because I literally used it like 2 min after I aspirated it.

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Re: Agar's GLC method problems! [Re: Bigbus]
    #14760992 - 07/13/11 05:43 AM (13 years, 6 months ago)

I use it immediately. :shrug:

Start over, no point in trying to figure this out.
Post pics as you go along.


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