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OfflineJlogan205
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Registered: 07/09/11
Posts: 13
Last seen: 11 years, 4 months
Cloning without a pressure cooker
    #14753439 - 07/11/11 08:29 PM (13 years, 6 months ago)

Hi, me again, I have a wild P. Cubensis and a I was thinking about cloning it. But, I happen to have a lack of a pressure cooker, so any advice would be great.

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OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 5 years, 3 months
Re: Cloning without a pressure cooker [Re: Jlogan205]
    #14754309 - 07/11/11 10:53 PM (13 years, 6 months ago)

Advice? Get a pressure cooker. :grin:

Even a small one is better than nothing, but I think the 23qt Presto is well worth the ~80$.

You'll want to read about agar, proper grain moisture, and making a still-air box. This is necessary, especially in your case, to clean a print/tissue before transferring it to grain. :goodluck:

:peace:


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Offlinethe astronaut
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Registered: 06/09/11
Posts: 447
Loc: usa Flag
Last seen: 6 months, 1 day
Re: Cloning without a pressure cooker [Re: Primal Call]
    #14754403 - 07/11/11 11:05 PM (13 years, 6 months ago)

A pressure cooker would be nice but not completely necessary. Look up pf without pressure cooker and ull find ur answers, side note I have never used one and it dont hurt anything just use trial and error like I did and ull see.

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Offlinetr8orjohn
Waiting for an airplane...
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Registered: 11/03/08
Posts: 162
Loc: Down in...Mexico
Last seen: 12 years, 29 days
Re: Cloning without a pressure cooker [Re: Primal Call]
    #14754409 - 07/11/11 11:06 PM (13 years, 6 months ago)

Could go with pre-poured agar in a glove box, if you have the $ and luck :shrug:

Once your deep enough into the hobby to start cloning though, you'll find more use for a PC then you might think


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Microbiologist turned mycologist, but still amateur enough to enjoy the occasional surprise...

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Terry M said:
Looking for rules that other people use successfully. Sterile procedure is just a set of rules that work.

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InvisibleWise Toad
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Registered: 06/08/10
Posts: 2,690
Re: Cloning without a pressure cooker [Re: tr8orjohn]
    #14754462 - 07/11/11 11:17 PM (13 years, 6 months ago)

Definitely get a PC, its worth so much more than the money to me. We need a PC smiley

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Offlinethe astronaut
Male


Registered: 06/09/11
Posts: 447
Loc: usa Flag
Last seen: 6 months, 1 day
Re: Cloning without a pressure cooker [Re: Wise Toad]
    #14754475 - 07/11/11 11:20 PM (13 years, 6 months ago)

pc :smile:, I wish I hade one.

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OfflineJlogan205
Stranger


Registered: 07/09/11
Posts: 13
Last seen: 11 years, 4 months
Re: Cloning without a pressure cooker [Re: the astronaut]
    #14754709 - 07/12/11 12:02 AM (13 years, 6 months ago)

Okay, I'm gonna try and find a cheap PC, and I'm just going to use the PF tek. I'm ordering some presterilized PF cake substrate jars from "everything mushrooms" for $4.50 a piece. Is there any way to use a spore print to do PF?

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Invisiblebabz89
engrossed:mycophile
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Registered: 07/07/09
Posts: 895
Loc: PNW! Flag
Re: Cloning without a pressure cooker [Re: Jlogan205]
    #14754868 - 07/12/11 12:49 AM (13 years, 6 months ago)

I got an 8 qt Which is pretty big (can fit 5 pint jars) for like 45$ at wal mart. Judt look they are well worth the buy!


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"When the war of love overcomes the love of war, the world will truely be a better places. One love"Bob Marley

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