|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Jlogan205
Stranger


Registered: 07/09/11
Posts: 13
Last seen: 11 years, 4 months
|
Cloning without a pressure cooker
#14753439 - 07/11/11 08:29 PM (13 years, 6 months ago) |
|
|
Hi, me again, I have a wild P. Cubensis and a I was thinking about cloning it. But, I happen to have a lack of a pressure cooker, so any advice would be great.
|
Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 5 years, 3 months
|
Re: Cloning without a pressure cooker [Re: Jlogan205]
#14754309 - 07/11/11 10:53 PM (13 years, 6 months ago) |
|
|
Advice? Get a pressure cooker. 
Even a small one is better than nothing, but I think the 23qt Presto is well worth the ~80$.
You'll want to read about agar, proper grain moisture, and making a still-air box. This is necessary, especially in your case, to clean a print/tissue before transferring it to grain. 
|
the astronaut



Registered: 06/09/11
Posts: 447
Loc: usa
Last seen: 6 months, 1 day
|
Re: Cloning without a pressure cooker [Re: Primal Call]
#14754403 - 07/11/11 11:05 PM (13 years, 6 months ago) |
|
|
A pressure cooker would be nice but not completely necessary. Look up pf without pressure cooker and ull find ur answers, side note I have never used one and it dont hurt anything just use trial and error like I did and ull see.
|
tr8orjohn
Waiting for an airplane...



Registered: 11/03/08
Posts: 162
Loc: Down in...Mexico
Last seen: 12 years, 29 days
|
Re: Cloning without a pressure cooker [Re: Primal Call]
#14754409 - 07/11/11 11:06 PM (13 years, 6 months ago) |
|
|
Could go with pre-poured agar in a glove box, if you have the $ and luck 
Once your deep enough into the hobby to start cloning though, you'll find more use for a PC then you might think
-------------------- --------------------------------------------------------------------------------------------------------
Microbiologist turned mycologist, but still amateur enough to enjoy the occasional surprise...
My Trade List
Terry M said:
Looking for rules that other people use successfully. Sterile procedure is just a set of rules that work.
|
Wise Toad


Registered: 06/08/10
Posts: 2,690
|
Re: Cloning without a pressure cooker [Re: tr8orjohn]
#14754462 - 07/11/11 11:17 PM (13 years, 6 months ago) |
|
|
Definitely get a PC, its worth so much more than the money to me. We need a PC smiley
|
the astronaut



Registered: 06/09/11
Posts: 447
Loc: usa
Last seen: 6 months, 1 day
|
Re: Cloning without a pressure cooker [Re: Wise Toad]
#14754475 - 07/11/11 11:20 PM (13 years, 6 months ago) |
|
|
pc , I wish I hade one.
|
Jlogan205
Stranger


Registered: 07/09/11
Posts: 13
Last seen: 11 years, 4 months
|
Re: Cloning without a pressure cooker [Re: the astronaut]
#14754709 - 07/12/11 12:02 AM (13 years, 6 months ago) |
|
|
Okay, I'm gonna try and find a cheap PC, and I'm just going to use the PF tek. I'm ordering some presterilized PF cake substrate jars from "everything mushrooms" for $4.50 a piece. Is there any way to use a spore print to do PF?
|
babz89
engrossed:mycophile



Registered: 07/07/09
Posts: 895
Loc: PNW!
|
Re: Cloning without a pressure cooker [Re: Jlogan205]
#14754868 - 07/12/11 12:49 AM (13 years, 6 months ago) |
|
|
I got an 8 qt Which is pretty big (can fit 5 pint jars) for like 45$ at wal mart. Judt look they are well worth the buy!
-------------------- "When the war of love overcomes the love of war, the world will truely be a better places. One love"Bob Marley
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 425 topic views. 20 members, 84 guests and 13 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|