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OfflineL-Shroom-D
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using grain to get more mycelium?
    #14744408 - 07/10/11 08:26 AM (13 years, 6 months ago)

So i have s little bag with colonized wbs. How can i use this to get the most out of it? Thanks


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InvisibleLeopardMan
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Re: using grain to get more mycelium? [Re: L-Shroom-D]
    #14744431 - 07/10/11 08:36 AM (13 years, 6 months ago)

Grain to grain transfers. Or a grain LC.


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Re: using grain to get more mycelium? [Re: LeopardMan]
    #14761833 - 07/13/11 11:16 AM (13 years, 6 months ago)

nice

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InvisibleEvilMushroom666
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Re: using grain to get more mycelium? [Re: LeopardMan]
    #14761987 - 07/13/11 11:42 AM (13 years, 6 months ago)

Quote:

LeopardMan said:
Grain to grain transfers. Or a grain LC.




:whathesaid:

OR

Grain to Agar, Grow out the culture, Agar to Grain, then G2G

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OfflineL-Shroom-D
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Re: using grain to get more mycelium? [Re: EvilMushroom666]
    #14762039 - 07/13/11 11:50 AM (13 years, 6 months ago)

Thank evil.

I think i will do agar:) seems best and i can view for any contams


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Re: using grain to get more mycelium? [Re: L-Shroom-D]
    #14762186 - 07/13/11 12:15 PM (13 years, 6 months ago)

G2G is awesome.  I was even able to colonize another grain bag with only 4 spawned rye berries.  The stuff just wants to grow.


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Re: using grain to get more mycelium? [Re: backstop]
    #14762213 - 07/13/11 12:19 PM (13 years, 6 months ago)

Well my mycelium is on colonized popcorn not grain :frown:

I hope i can do agar with popcorn.


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Invisiblewildernessjunkie
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Re: using grain to get more mycelium? [Re: L-Shroom-D]
    #14762260 - 07/13/11 12:26 PM (13 years, 6 months ago)

Quote:

L-Shroom-D said:
Well my mycelium is on colonized popcorn not grain :frown:

I hope i can do agar with popcorn.




Corn = Grain

Anything that is a grass too. So Rye, wheat, corn, milo, millet, rice...All grains. Im sure there are a few others that dont come to mind right off.

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InvisibleEvilMushroom666
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Re: using grain to get more mycelium? [Re: L-Shroom-D]
    #14762267 - 07/13/11 12:27 PM (13 years, 6 months ago)

FYI: Popcorn is considered a grain. As is Millet, most of the ingredients
in WBS, rye, wheat, barley etc.

You can grain to grain with any fully colonized grain, be it WBS, rye,
wheat, etc etc.

Place one kernel of colonized popcorn onto agar and within 24-48 hours
it should start to recover into the agar.

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Re: using grain to get more mycelium? [Re: L-Shroom-D]
    #14762277 - 07/13/11 12:28 PM (13 years, 6 months ago)

popcorn is a grain :gethigh:
Also, grain LCs are always crystal clear when using popcorn.
Good luck


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OfflineL-Shroom-D
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Re: using grain to get more mycelium? [Re: LeopardMan]
    #14762327 - 07/13/11 12:38 PM (13 years, 6 months ago)

Fyck yeah! You guts made my day:)

Buying some agar petris now. Time to make a bunch of dishes. Then i can make grain lc for sure.

What will colonize grain quicker    lc or agar wedge?


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InvisibleEvilMushroom666
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Re: using grain to get more mycelium? [Re: L-Shroom-D]
    #14762346 - 07/13/11 12:42 PM (13 years, 6 months ago)

IMO Agar because of the following reasons:

Agar > Grain:
Colonize plate, take plate and under sterile conditions transfer wedges
to your grain donor jars. Let jars grow out 5-6 days before giving the
jar a good shake. Jar should finish up around day 14.

LC > Grain
Transfer agar to LC, grow out LC for 10-14 days, shoot a test jar/plate
of agar to make sure your LC is clean (7-10 days) then take your LC and
inoculate your grain jars. A good strong LC will colonize grain surprisingly
fast, but you loose a lot more time preparing and testing the LC then
if you just went agar to grain.

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