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saltyd
Stranger
Registered: 05/13/11
Posts: 62
Last seen: 3 years, 18 days
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I keep filtering the LC and sediment keeps showing up!
#14738941 - 07/08/11 11:39 PM (12 years, 7 months ago) |
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I'm not sure what the best was is to filter a liquid culture. I boiled the water, mixed in the malt extract and dextrose, let it cool, and filtered it. The filter didn't look like it took out too much. Then I pressure cooked it and bottled it and put it aside, and the next day it was full of sediment. Is this some sort of slow forming sediment, or how do I get it all out before I start to PC?
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iDmt
Humbled


Registered: 05/19/11
Posts: 686
Last seen: 9 years, 6 months
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Re: I keep filtering the LC and sediment keeps showing up! [Re: saltyd]
#14738971 - 07/08/11 11:48 PM (12 years, 7 months ago) |
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Could be crystalized sugars after pcing I'm not experienced whatsoever in LC just throwin it out there
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BeanBurner
Stranger
Registered: 07/15/09
Posts: 41
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Re: I keep filtering the LC and sediment keeps showing up! [Re: saltyd]
#14739019 - 07/09/11 12:01 AM (12 years, 7 months ago) |
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As the LC cools, the absorption rate of the water decreases. That is why you are seeing sediment the next day. I do not claim to be an expert on this and have only gotten 2 LC's to work so far and have tried many. I would re-filter with 2 coffee filters and re-PC, allow it to cool and see if any sediment forms. If so, repeat the process until no sediment. You can use this information to systematically reduce the amount of LME/Dex you use at the start until you have a formula that works for you that will not produce sediment and will viable after 1 round of PC.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: I keep filtering the LC and sediment keeps showing up! [Re: BeanBurner]
#14741365 - 07/09/11 03:00 PM (12 years, 7 months ago) |
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Sediment is fine, the myc loves it.
But LC from spores is a gamble, get a pack of agar for $1.69 and win big.
-------------------- You make it all possible. Doesn't it feel good?
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saltyd
Stranger
Registered: 05/13/11
Posts: 62
Last seen: 3 years, 18 days
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Re: I keep filtering the LC and sediment keeps showing up! [Re: Doc_T]
#14743104 - 07/09/11 10:41 PM (12 years, 7 months ago) |
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Can you make a liquid culture from agar then? I thought you could only make it from spores...
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scatmanrav
Brainy Smurf


Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 1 month
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Re: I keep filtering the LC and sediment keeps showing up! [Re: saltyd]
#14743239 - 07/09/11 11:20 PM (12 years, 7 months ago) |
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Drop a wedge of agar into LC, bam, LC from agar. You can make LC from anything, a peice of grain, cake, mushroom, agar.
The PC makes more sediment, since it makes it above boiling temps, more crystalization. But yeah, shouldnt matter.
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