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insutama
No pain no gain


Registered: 11/24/08
Posts: 1,207
Loc: Canada
Last seen: 3 years, 7 months
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My grain progress and questions
#14740555 - 07/09/11 11:29 AM (13 years, 6 months ago) |
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Okay so as you may all know this is my first grain grow. I originally inoculated 4 1 pint jars of rye grain. For two of the jars i used a spore print that i took on tinfoil from my last grow and for the other two jars i used a spore print that my buddy took on a plastic petri dish. out of the 4 jars 2 of them got contams. Both jars that got contams were from my buddys print he took on the plastic petri dish so im thinking it was a print issue. The other two jars are going good tho as you can see in the pics.
   
the one on the right is one of the contams Now a few questions. Should i shake my jars at about 25% colonization or would that slow down the growth seem as the mycelium would have to refortify ?
Do you think that the reason my buddys prints never worked was because it was taken on a plastic petri dish rather than tinfoil ?
Edited by insutama (07/09/11 11:30 AM)
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Re: My grain progress and questions [Re: insutama]
#14740579 - 07/09/11 11:36 AM (13 years, 6 months ago) |
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Yes, I shake at 20-30%, again at around 60%, and then right before I use them as spawn
What are your lids like? I ask because I havent seen a contam in a long time, any idea why that happened
Dont know if the petri is responsible but I do think that some heavy duty tin foil would be best
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insutama
No pain no gain


Registered: 11/24/08
Posts: 1,207
Loc: Canada
Last seen: 3 years, 7 months
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Re: My grain progress and questions [Re: Wise Toad]
#14740613 - 07/09/11 11:42 AM (13 years, 6 months ago) |
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My lids have 2 1/8" holes in filled with poly fill. Im just thinking it the petri dishes because there plastic so you cant PC cook them all he did was wipe them down with alcohol. plus the plastic would probally hold bacteria better than foil would. anyway ill be continuing to use foil for my prints so far every print i have taken has not had contams this is my first 2 contams and it came from his print
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steelmonkey
Homejigger



Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada
Last seen: 3 years, 3 months
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Re: My grain progress and questions [Re: Wise Toad]
#14740616 - 07/09/11 11:43 AM (13 years, 6 months ago) |
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There could be a ton of reasons why your buddies print could be contaminated,no spore print is completely sterile no matter how its made.Best to grow it out on agar to see if the print is the issue
I shake at around 30% I don't see the need to shake again until I break it up for spawning,if its healthy vigorous myc it will rip through the grains after the first shake
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King Cap
Beginning Mycologist



Registered: 02/22/11
Posts: 483
Loc: South Park Colorado
Last seen: 13 years, 2 months
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Re: My grain progress and questions [Re: steelmonkey]
#14740739 - 07/09/11 12:11 PM (13 years, 6 months ago) |
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Yea only shake once. Ive read posts that say not to shake but it made a HUGE difference in how fast my jars colonized
-------------------- "Snow is like sex. You never know how much your gonna get or how long it's gonna last"
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Re: My grain progress and questions [Re: King Cap]
#14740764 - 07/09/11 12:17 PM (13 years, 6 months ago) |
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The reason I say shake again at 60% is because I often find that large sections of the rye are uncolonized even when I shake the hell out of them. We need a shaking grain jar smiley and a mycelium on grain smiley
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steelmonkey
Homejigger



Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada
Last seen: 3 years, 3 months
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Re: My grain progress and questions [Re: Wise Toad]
#14740783 - 07/09/11 12:24 PM (13 years, 6 months ago) |
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I agree we do need we do need a shaking grain smiley
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 11 months
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Re: My grain progress and questions [Re: insutama]
#14740816 - 07/09/11 12:33 PM (13 years, 6 months ago) |
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Why did you only fill the jars 1/2way and why did you use pints? Are you doing GLC or something. I would inject a shit ton of sterile water in there and do GLC per Agar's tek.
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Wavy Cap Cyan Spore Prints for Trade/Sale
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insutama
No pain no gain


Registered: 11/24/08
Posts: 1,207
Loc: Canada
Last seen: 3 years, 7 months
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Re: My grain progress and questions [Re: OregonChronic]
#14741936 - 07/09/11 05:21 PM (13 years, 6 months ago) |
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i didnt have enough grain to fill up the jars 3/4s the way
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