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afrogus
hombre



Registered: 01/23/11
Posts: 949
Loc: Cali
Last seen: 14 hours, 28 minutes
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Shit! Are my LC's contaminated......
#14735610 - 07/08/11 10:36 AM (13 years, 6 months ago) |
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Its been about 2 and 1/2 weeks and there seems to be some stuff in my lc. In one of them there are 2 red dots. In the others some black dots and I think some other dark wispy schmeg. Am I crazy or what?
-------------------- "Leave no turn unstoned":)
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lostinbq
Clap...or we will find u!!!!


Registered: 03/25/11
Posts: 525
Loc: ZombieLand
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Re: Shit! Are my LC's contaminated...... [Re: afrogus]
#14735622 - 07/08/11 10:40 AM (13 years, 6 months ago) |
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how were they made? with a MS syringe? wont know till you try it.
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myc0nob
Philosopher

Registered: 06/20/11
Posts: 118
Last seen: 2 months, 27 days
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Re: Shit! Are my LC's contaminated...... [Re: lostinbq]
#14735793 - 07/08/11 11:22 AM (13 years, 6 months ago) |
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I made some really nice cobweb jars with a LC that had lots of tiny black dots in them, but guess a test jar is the only way to know
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lostinbq
Clap...or we will find u!!!!


Registered: 03/25/11
Posts: 525
Loc: ZombieLand
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Re: Shit! Are my LC's contaminated...... [Re: myc0nob]
#14735942 - 07/08/11 11:53 AM (13 years, 6 months ago) |
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my first lc had tiny black dots but it was made from a MS. made 10 jars from it and another lc witch was proven viable. just have to test them.
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afrogus
hombre



Registered: 01/23/11
Posts: 949
Loc: Cali
Last seen: 14 hours, 28 minutes
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Re: Shit! Are my LC's contaminated...... [Re: lostinbq]
#14737111 - 07/08/11 04:22 PM (13 years, 6 months ago) |
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I made them with an MS syringe using corn syrup. But the corn syrup (Karo) was flavored with vanilla and had a little salt in it too.
-------------------- "Leave no turn unstoned":)
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Immortal Corrupter
Elevator Eater



Registered: 06/08/11
Posts: 424
Last seen: 6 years, 1 month
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Re: Shit! Are my LC's contaminated...... [Re: afrogus]
#14737275 - 07/08/11 04:55 PM (13 years, 6 months ago) |
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Karos fine man, I've used it with no problems. Are the black dots completely new or where they in the syringe beforehand? I've had a few lcs contam so just trying to compare
-------------------- where's my lap dance?
.
/OO\
/ (y) \
//\
(\\__/)
( O.o )
( > < )
What poor gods we do make
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Karo is ok, honey is better.
But LC from spores is a gamble every time. When it pays off it's great. When it doesn't, get agar. $1.69 well spent.
-------------------- You make it all possible. Doesn't it feel good?
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afrogus
hombre



Registered: 01/23/11
Posts: 949
Loc: Cali
Last seen: 14 hours, 28 minutes
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Re: Shit! Are my LC's contaminated...... [Re: Doc_T]
#14737437 - 07/08/11 05:38 PM (13 years, 6 months ago) |
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The black spots have me less worried then the 2 tiny red dots floating amongst my cloud in the PE jar. The black spots were in the syringe I imagine. The black wispy appearance is new, like in the last day or so, same with the red dots.
-------------------- "Leave no turn unstoned":)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Shit! Are my LC's contaminated...... [Re: afrogus]
#14737477 - 07/08/11 05:49 PM (13 years, 6 months ago) |
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Toss, start agar dishes. PF jars work great to get started.
-------------------- You make it all possible. Doesn't it feel good?
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Doctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc:
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Re: Shit! Are my LC's contaminated...... [Re: afrogus]
#14737518 - 07/08/11 05:59 PM (13 years, 6 months ago) |
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LC's are best assumed contaminated, UNTIL, test substrates have been inoculated and have had 5 days of growth with NO contamination.
Best to forget LC's all together and learn agar, (as suggested above) inoculating sterilized grain mediums with clean agar culture.
Heck, it's FAR superior to inoculate sterile grain mediums with known MS syringes from a reputable vendor, expanding healthy grain spawn jars with a process called grain to grain transfers over LC's.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Shit! Are my LC's contaminated...... [Re: Doctor_Inoc]
#14737528 - 07/08/11 06:02 PM (13 years, 6 months ago) |
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Agreed, with an amendment: 
Quote:
Doctor_Inoc said: LC's are best assumed contaminated, UNTIL, test substrates have been inoculated and have had 5 days of growth with NO contamination been taken all the way to fruiting.
-------------------- You make it all possible. Doesn't it feel good?
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Immortal Corrupter
Elevator Eater



Registered: 06/08/11
Posts: 424
Last seen: 6 years, 1 month
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Re: Shit! Are my LC's contaminated...... [Re: Doc_T]
#14737728 - 07/08/11 06:52 PM (13 years, 6 months ago) |
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Yeah, black wisps definately not good. I'd just start all over.
Agars fine if you can get it, but if your ass is unlucky, an LC will work fine. I use both honey and karo, but karo is definately nicer for spotting contams.
-------------------- where's my lap dance?
.
/OO\
/ (y) \
//\
(\\__/)
( O.o )
( > < )
What poor gods we do make
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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You can get agar, that's not the issue. People think it's expensive, or difficult to work with, or too advanced. It's none of those.
-------------------- You make it all possible. Doesn't it feel good?
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Immortal Corrupter
Elevator Eater



Registered: 06/08/11
Posts: 424
Last seen: 6 years, 1 month
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Re: Shit! Are my LC's contaminated...... [Re: Doc_T]
#14742414 - 07/09/11 07:23 PM (13 years, 6 months ago) |
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-------------------- where's my lap dance?
.
/OO\
/ (y) \
//\
(\\__/)
( O.o )
( > < )
What poor gods we do make
|
Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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What part of the world are you in?
-------------------- You make it all possible. Doesn't it feel good?
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Immortal Corrupter
Elevator Eater



Registered: 06/08/11
Posts: 424
Last seen: 6 years, 1 month
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Re: Shit! Are my LC's contaminated...... [Re: Doc_T]
#14742539 - 07/09/11 07:52 PM (13 years, 6 months ago) |
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US, its not like I haven't looked, I've even checked bulk and health food stores.
-------------------- where's my lap dance?
.
/OO\
/ (y) \
//\
(\\__/)
( O.o )
( > < )
What poor gods we do make
|
Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Did you ask? Go to a local non-chain health store, and ask for agar.
-------------------- You make it all possible. Doesn't it feel good?
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