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Amazon Shop: ½ Pint Jars, Paul Stamets

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Registered: 04/19/03
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Slowing Incubation and Maximum Incubation Times
    #1473460 - 04/19/03 04:51 PM (15 years, 8 days ago)

Someone I met woud like to know whether variations in temperature can be used to control the total incubation time for a p. cubensis culture. He said that he knew mycelium growth slowed with lower temperatures, but he didn't know whether there was a total incubation time past which the mycelium must either fruit or die.

An example scenario: Mr. X has an incubation jar around two weeks old. For whatever reason, Mr X doesn't want to have to birth this jar for another month, though mycelium has already spread to ~80% of the jar. Can Mr X slow the growth enough by lowering the temperature to make the incubation period a month longer without harming the mycelium? How long can a culture stay in a jar, after first signs of pinning? Can growth be halted completely through refridgeration, thus keeping the culture in a suspended state? If so, for how long?

Again, thanks.

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Re: Slowing Incubation and Maximum Incubation Times [Re: Cull]
    #1473556 - 04/19/03 05:22 PM (15 years, 8 days ago)

yes, you can put it in the fridge to slow growth without harming the mycelium.
as for whether or not there is a limit to the amount of time spent colonizing,
common sense would say yes, because the mycelium will eventually use up all
the nutes in the jars, but im no expert.

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Re: Slowing Incubation and Maximum Incubation Times [Re: flow]
    #1473599 - 04/19/03 05:42 PM (15 years, 8 days ago)

Paul Stamets claims to have viable strains of myc in his fridge that are 20 years old. I think he shoots for 33F. I've kept fully colonized quarts of grain for several months in a normal household fridge, and it was fine.

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Re: Slowing Incubation and Maximum Incubation Times [Re: RogerRabbit]
    #1477335 - 04/20/03 10:04 PM (15 years, 7 days ago)

i have used the fridge (33F) to maintain spawn masters until i needed them. that is beside the point i want to add which would be: I think you would do yourself a service to refridgerate a fully colonized substrate as opposed to using refridgeration to slow colonization of an incompletely colonized substrate. just wasn't clear on your intentions there.

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Re: Slowing Incubation and Maximum Incubation Times [Re: debianlinux]
    #4495347 - 08/04/05 03:43 PM (12 years, 8 months ago)

cover your jars with some saran wrap and a rubber band or some coffee filters or something. I have lost jars that were fine when they were done colonizing then taken over after sitting the fridge for a month.

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Amazon Shop: ½ Pint Jars, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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