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OfflinePizzydizzler
Stranger
Registered: 05/22/11
Posts: 17
Last seen: 13 years, 5 months
No Growth, so can I noc again?
    #14727685 - 07/06/11 06:44 PM (13 years, 6 months ago)

Another topic that I couldnt get any worthwhile results on through the search engine.

Anyway my friend has 12 jars (P Cubies), 6 are showing mycelial growth.  Of the 6 with growth 3 were g2g and are looking fantastic.  The other 3 are MS and are a bit slow but getting there.  Anyway the other 6 have been sitting around for about 2 weeks since they got nocced up with a MS syringe and they have shown no growth.

My question is can she take these unsuccesful jars and noc them up with a g2g transfer.  The Mazatapec that she has been using has been very succesful so she wants to take a fully colonized jar and use it to colonize all the jars that have shown no growth since being sprayed with spores 2 weeks ago. 

Would this cause problems if these jars with no growth had a different strain (mazatapec grain) introduced to them?  She can always start over but these jars are very good and shed rather just use them as is if she could.

Thanks for your help and I hope this thread helps other as well!


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"Never bring a sandwich to a buffet"

"Live every week like its shark week"

"Dress everyday like you are going to get murdered in those clothes"

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OfflinetracingBigTime
Mad Mycologist
Male User Gallery


Registered: 08/21/10
Posts: 143
Last seen: 2 years, 7 months
Re: No Growth, so can I noc again? [Re: Pizzydizzler]
    #14727723 - 07/06/11 06:52 PM (13 years, 6 months ago)

I have heard that it is best to throw away, but you can re-sterilize.  I have re-inoculated jars, but only if going w/same strain.  I have had no problems. 

GL


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<<<<<<<----TRACING---->>>>>>>>

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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: No Growth, so can I noc again? [Re: Pizzydizzler]
    #14727736 - 07/06/11 06:55 PM (13 years, 6 months ago)

How is the lid designed for GE?

Does the grain appear dry?

Why gamble a qt of GOOD spawn on questionable grain?


--------------------
“Sacred cows make the best hamburger”

Mark Twain



Independant Research Foundation

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OfflinePizzydizzler
Stranger
Registered: 05/22/11
Posts: 17
Last seen: 13 years, 5 months
Re: No Growth, so can I noc again? [Re: Carl Sagan]
    #14729220 - 07/06/11 11:43 PM (13 years, 6 months ago)

Very true that it is a gamble but these jars that arent working were nocced up by some very questionable syringes from spore works or whatever.  I think only two attempts out of like 15 with sporeworks has actually shown growth, so  am pretty damn sure that the grain jars are fine and that it is the syringes.  Its possible that the ones showing no growth didnt even have a single live spore in them or that if it did they did not last in the grain.

The grain does not appear to dry, although it does look dryer than the past few that have been succesful, although those were a bit too wet.  Searching for the perfect middle ground. 

As for the lid I do not know what GE means but I am guessing it means Grain Entry or something like that.  The lid is a lid but considering she has done something like 10 g2g transfers with nothing but fantastic results in not even half sterile environments I think the grain transfer could be done safely. 

Thanks for the advice and anymore help would be great.

She basically wants to know if it is possible to take a jar that has not colonized but lets say was technically inoculated with PF Classic, shows no growth, and then gets some Mazatapec grains thrown in it.

  Even if the PF Classic was not dead and just slow would the two strains clash or would the stronger one simply take over?

By the way she strongly suggests getting the Mazatapec strain from Lil' Shop of Spores.  She ordered spores101 and then later that day read all the bad reviews so she grabbed some from LSoS and they threw in the Maz. for free. She thought they were just giving her an overstocked shitty one but too her surprise it is one of the main reasons she is having such success on her first grow!

[Edited: Accidentally said sporeworks was the bad vendor but it was actually Spores101 with the shitty syringes, basically 4 out of 6 syringes sucked with maybe no spores at all.]

Edited by Pizzydizzler (07/13/11 03:05 AM)

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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: No Growth, so can I noc again? [Re: Pizzydizzler]
    #14729248 - 07/06/11 11:52 PM (13 years, 6 months ago)

Quote:

As for the lid I do not know what GE means but I am guessing it means Grain Entry or something like that.



GE=Gas Exchange

Quote:

she has done something like 10 g2g transfers with nothing but fantastic results in not even half sterile environments I think the grain transfer could be done safely.



"Even the blind squirrel finds a nut every now, and again"


--------------------
“Sacred cows make the best hamburger”

Mark Twain



Independant Research Foundation

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OfflinePizzydizzler
Stranger
Registered: 05/22/11
Posts: 17
Last seen: 13 years, 5 months
Re: No Growth, so can I noc again? [Re: Carl Sagan]
    #14729266 - 07/06/11 11:57 PM (13 years, 6 months ago)

Ahh    the gas exchange is better than all the succesful jars from before.  And yeah the sterile environment isnt perfect,  but she is very careful and uses lots of lysol and what not.  Ten for ten takes a pretty skilled squirell.

Im not looking for advice on how to g2g just seeing if this attempt is even possible
But yeah the question I am asking is if it is possible to add Maz to a jar that has been innoced with some other strain.  Ill worry about the chances involved with doing a g2g.


--------------------
"Never bring a sandwich to a buffet"

"Live every week like its shark week"

"Dress everyday like you are going to get murdered in those clothes"

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OfflineCrushed
Sober Enough


Registered: 02/18/10
Posts: 96
Loc: Southwest
Last seen: 13 years, 5 months
Re: No Growth, so can I noc again? [Re: Pizzydizzler]
    #14729433 - 07/07/11 12:41 AM (13 years, 6 months ago)

If a jar isn't growing, there is a reason. Identifying the reason with certainty can be almost impossible, but you know something is wrong. If the innoculant was bunk, reinnoculating would be fine. If there is anything wrong with the conditions inside the jar, or a contamination, reinnoculating would be a waste of time and money.

Given how cheap jars are to prepare, I personally would much rather put in ~1 hr or so and remake them, rather than risk wasting innoculant and a week or more of time.


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In theory, there is no difference between theory and practice.
In practice, there is.

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Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: No Growth, so can I noc again? [Re: Crushed]
    #14729471 - 07/07/11 12:54 AM (13 years, 6 months ago)

As said before, the conditions are the primary issue. For the very low price of Grain, I would just prepare new jars, and g2g them with one of the known good ones.

Crossing the strains wont hurt anything either. You just end up with prints that you cant label as one strain or another. The mushrooms however don't care what you call them and will grow anyway.

Also, Sporeworks has got an excellent reputation. I think you may be confusing them with a few other vendors out there that are not Shroomery sponsors. (Spores101 probably)

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OfflinePizzydizzler
Stranger
Registered: 05/22/11
Posts: 17
Last seen: 13 years, 5 months
Re: No Growth, so can I noc again? [Re: wildernessjunkie]
    #14729484 - 07/07/11 12:59 AM (13 years, 6 months ago)

yes i definately meant spores 101    Good Call, dont want to screw with works rep.


--------------------
"Never bring a sandwich to a buffet"

"Live every week like its shark week"

"Dress everyday like you are going to get murdered in those clothes"

Extras: Filter Print Post Top
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