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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 11 months
should i make another grain LC.
    #14728287 - 07/06/11 08:58 PM (13 years, 6 months ago)

Well I know the answere already but I was wondering if anyone has had a problem like this.  See i shot up some grain with MS and it show signs of growth the next day.  I let them go for a little bit longer, then shook and they were ready a week later.  The jars were colonized beautifully I inspected each one throughly and they were all free of nasties.  I took one of them and  made  a grain LC.  So now I have nice big jar of LC.  I let the jar recolonize.  For being really wet it colonized nicely.  So its fully colonized now and I was looking at it the other day and it appears that it has some of the green funk forming on the top of it.  So after seeing that Im thinking that means that my LC has the funk in it, so im going to make some more LC with another one of the remaining jars. Its crazy though because the jar I used had absolutley zero signs of anything bad in it.  Just wondering if anyone has had a hiccup like this before.  Thanks


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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: should i make another grain LC. [Re: JonnyLongboard]
    #14728364 - 07/06/11 09:09 PM (13 years, 6 months ago)

One week to full colonization from MS?  :confused2:
I don't think you were growing Cubensis mycelium.


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Offlineafrosheen
9Lives the cat
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Registered: 03/06/10
Posts: 1,878
Last seen: 3 years, 3 months
Re: should i make another grain LC. [Re: Carl Sagan]
    #14728854 - 07/06/11 10:31 PM (13 years, 6 months ago)

One day to show growth on grains from a MS inoculation?

Naw dude. It was unhealthy to start with. Most of the time with spores you'll wait 5-7 days to see ANYTHING on grains. You've had trich since day one. Re-examine your sterile techniques and try again.


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InvisibleTranscendingLife
I Don't Need a Life to Live
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Registered: 06/09/10
Posts: 21,627
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Re: should i make another grain LC. [Re: afrosheen]
    #14728906 - 07/06/11 10:40 PM (13 years, 6 months ago)

You said the grains were wet. There's your first problem.

Did you get the syringe form a reputable source?

Sounds like you took a few short cuts.

Soak for 24
BOIL (not simmer) for 6-10 minutes, stirring starting @ 180F.
Toss in strainer for 30-60 minutes
PC @ 15 PSI for 90-120 minutes
Let cool over night
Inoculate.

I use pint jars 1/2 filled for GLCs. I specifically make 1 jar for them. The rest I use to G2G or spawn to bulk.

I use 1cc of the GLC (after being tested) to inoculate an LC & use that for the clean master.

Well, sorry for the long answer.

In short:

Start your GLC over again & watch yours jars...I bet they'll go bad soon.

Pictures would help identify if there's contams in there or not.

Here's a healthy (tested & true) GLC 2 weeks after the extaction:


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Edited by TranscendingLife (07/06/11 10:47 PM)

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OfflineRogerRabbitM
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Re: should i make another grain LC. [Re: JonnyLongboard] * 1
    #14729050 - 07/06/11 11:06 PM (13 years, 6 months ago)

Quote:

JonnyLongboard said:
See i shot up some grain with MS and it show signs of growth the next day.




I didn't have to read past that to know you were growing mold.
RR


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OfflineJonnyLongboard
Habadasher

Registered: 04/19/10
Posts: 126
Loc: Yeti compound
Last seen: 11 years, 11 months
Re: should i make another grain LC. [Re: RogerRabbit]
    #14733425 - 07/07/11 08:55 PM (13 years, 6 months ago)

Quote:

TranscendingLife said:
You said the grains were wet. There's your first problem.

Did you get the syringe form a reputable source?

Sounds like you took a few short cuts.

Soak for 24
BOIL (not simmer) for 6-10 minutes, stirring starting @ 180F.
Toss in strainer for 30-60 minutes
PC @ 15 PSI for 90-120 minutes
Let cool over night
Inoculate.







I said after I did the grain LC I allowed the wet jar that I used to recolonize.  If youve done grain LC before you would know that after you take the water back out of the grain jar the grains are wet. Thats what im mean when I say wet. 
Yes the syringe is from a reputable source. T.H.E.
I took zero shortcuts my grain prep is spot on.
1. Very thorough rinse.
2. 24 hour soak
3. Rinse again
4. Boil for about 10 mins.
5. Pour steaming grain into strainer and allow it to steam off.  Tossing it every few minutes for 20 minutes until grain is dry on the outside.
6. Load into jars. Foil over top.
7. Pressure cook for 90 minutes.
8. Allow to cool completly
Now there ready to shoot up.

As far as you guys saying that I was growing mold. Your wrong. I might not be a master mycologist but I have went through alot of trial and error.  And Ive been learning every step of the way.  When I say that I saw growth 24 hours after shooting them up Im talking about an almost microscopic strand of mycelium. I checked these jars daily and watched the beautiful thick snow white rizomorphic mycelium form day after day.  I let them go a little while then I shook and they were fully colonized a week later.  Im know that it is P.C. mycelium. 100% sure. I did not grow mold. Its mycelium guranteed.  My question again is, has anyone done a grain LC to a perfectly healthy looking jar and after allowing the jar to recolonize you had some trich in it?  Thank you all


--------------------
"Lets get down to brass tacks, how much for the ape?"

Everything I say on this forum is completly theorectical. A complete fictionilization.

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