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OfflineOregonChronic
Iron, Lion, Cyans
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Registered: 09/12/10
Posts: 1,970
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Spore Printing in a Pint Jar
    #14727457 - 07/06/11 05:48 PM (13 years, 6 months ago)

I have a fresh pack of new pint jars i bought and i was wondering if i could just wipe a blade or scissors or whatever with iso and just cut the cap off and drop into a fresh un opened pint jar inside a glovebox. Are brand new pint jars sterile to take a spore print in? Or should i PC it before i do it? The cap of desire is dropping spores a lot right now.

Im just gonna print it in the jar, and then squirt like 120cc's sterile PC'd water in there and shake then aspirate it all back out with 12cc syringes one at a time.


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Offlinemabachko
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Registered: 02/11/11
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Re: Spore Printing in a Pint Jar [Re: OregonChronic]
    #14727499 - 07/06/11 05:58 PM (13 years, 6 months ago)

I use them right out of the box.

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OfflineOregonChronic
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Registered: 09/12/10
Posts: 1,970
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Last seen: 11 years, 11 months
Re: Spore Printing in a Pint Jar [Re: mabachko]
    #14730582 - 07/07/11 10:48 AM (13 years, 6 months ago)

I kinda messed up...i didn't scrape the spores off the glass before i added the water and basically i had to open the jar up(in a glovebox) and i took a butter knife,...iso'd it down, and scraped em up with just an iso'd butter knife blade and put the lid back on now im bout to aspirate it back into several syringes to inoc several rye jars to see if its contamd or not. I should get agar and some petri dishes. Im not really about spore printing. The chance for contam is just too high. Im really interested in GLC and cant wait to get some going. I hate waiting for MS to germ. But,...gotta start somewhere. Thats why im cloning some tissue to 2 GLC jars today. Get shit goin. Dont wanna waste a bunch of rye/jars/time on some MS inocs that take forever/might be contamd because its from spores that my sterile technique wasn't all that good during printing/making the syringes. IMO GLC is the way to go. Talk about multiplying cultures. :super::mushroomgrow:


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