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OfflineGigabyt3r
Stranger

Registered: 05/24/11
Posts: 58
Last seen: 13 years, 6 months
BRF 1 Month After Innoc ... Getting Stressed!
    #14714115 - 07/04/11 03:02 AM (13 years, 6 months ago)





These are my BRF jars after 1 month ... no visible growth of either myc or contam? Why the hell are they not doing anything? Temp is around 80 constant in a dark TiT.



Also my WBS jars were inoculated around 6 days ago, I noticed some growth this morning but it doesn't look like Mycelium, I just wondered what peoples thoughts were?

Also heres a picture of my LC, about the only success iv'e had so far (and thats probably contaminated knowing my luck)



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"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"

<3 Shiitake's

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Invisiblebbox244
Legally Insane
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Registered: 03/17/11
Posts: 205
Loc: USA Flag
Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: Gigabyt3r]
    #14714203 - 07/04/11 03:53 AM (13 years, 6 months ago)

With LC, you would see growth in 2-3 days.  Your LC is contaminated.  The only other possibility is that every substrate you made is contaminated with bacteria.  But, thats not probable.  It's likely that your LC was contaminated with bacteria, or perhaps a fungus.  Sorry to be the bearer of bad news, but I doubt you messed up on all your substrate.  In any case, you could put a drop on an agar plate and look at the growth.  Otherwise, if you have doubts about your sterile procedure, you can always try again, but if I were you I would take my spore syringe (assuming thats what you have), and try a MS inoculation to WBS, then when colonized, make a GLC.  I gave up on sugar water, and only do GLC's now.  Never looked back.  Good luck.


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.... Always curious, always looking for better ways, mind always wonders ....

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Edited by bbox244 (07/04/11 03:54 AM)

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OfflineGigabyt3r
Stranger

Registered: 05/24/11
Posts: 58
Last seen: 13 years, 6 months
Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: bbox244]
    #14714245 - 07/04/11 04:29 AM (13 years, 6 months ago)

Sorry I wasn't very clear about the last bit, I only shot up my LC 5 days 6 days ago and saw growth within 2. Thanks for the reply I'll look into MS inoculation as i'm not sure what it is.


--------------------
"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"

<3 Shiitake's

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OfflineNormalus
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Registered: 09/23/10
Posts: 23
Last seen: 13 years, 5 months
Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: Gigabyt3r]
    #14714270 - 07/04/11 04:52 AM (13 years, 6 months ago)

And what is GLC? i can`t find anything about it in this forum. MS is multispore.

http://www.shroomery.org/9423/Mycological-and-OMC-abbreviations


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Offlineveda_sticks
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Registered: 07/29/07
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Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: Normalus]
    #14714327 - 07/04/11 05:23 AM (13 years, 6 months ago)

If none of your brf jars grew anything then it could be down to a bad syringe. you did get it from a shroomery sponsor?

www.mushroomvideos.com

follow the pf tek there.

Also those jars look quite large, you really want as close to 1/2 pint wide  mouth as you can get.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinekdmmontana
Male

Registered: 11/09/10
Posts: 1,114
Last seen: 12 years, 6 months
Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: Gigabyt3r]
    #14714502 - 07/04/11 07:13 AM (13 years, 6 months ago)

Looks like your BRF Jar is suffocated. A good tip; take a couple of smaller jars and work with the reciped a bit, finding something thats balanced and right to the eye(and follow the TEK). A too wet substrate is not good as well as a too airy one. Yours look a bit slushy and pasty and that can cause oxygen issues.

Take a look at mine for a second:)


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OfflineGigabyt3r
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Registered: 05/24/11
Posts: 58
Last seen: 13 years, 6 months
Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: kdmmontana]
    #14714596 - 07/04/11 08:00 AM (13 years, 6 months ago)

Quote:

kdmmontana said:
Looks like your BRF Jar is suffocated. A good tip; take a couple of smaller jars and work with the reciped a bit, finding something thats balanced and right to the eye(and follow the TEK). A too wet substrate is not good as well as a too airy one. Yours look a bit slushy and pasty and that can cause oxygen issues.

Take a look at mine for a second:)







I did add a little extra water when following the PF tek as I thought it looked a little dry, wish I hadn't now! Is there any way I can rectify this? Dry it out a little without opening it up to contams?

It would be nice euse these jars rather than starting afresh


--------------------
"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"

<3 Shiitake's

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Offlinekdmmontana
Male

Registered: 11/09/10
Posts: 1,114
Last seen: 12 years, 6 months
Re: BRF 1 Month After Innoc ... Getting Stressed! [Re: Gigabyt3r]
    #14714701 - 07/04/11 08:47 AM (13 years, 6 months ago)

You have to re-do your jars, I am sorry. 1 month is far too long waiting for germination.

Adding extra water is NEVER needed (Ive had such thoughts as well) The most common reason for stalled or stumped growth is oxygen depravation caused by water. Your substrate is not thoroughly mixed and has too much water in it. The BRF can clump up and cause oxygen issues.

My best tip is to work hard on the substrate and go for something thats light and fluffy. Mind that the BRF has to be flour grade, meaning do not do the dumb mistake of grinding your BRF half way or use whole rice kernels. Ive heard this from some people but I would not go for it..

First, make sure you use a finer grade of Verm, (gravel size is perfect) as this will make it easier to mix the substrate all together. Coarser substrates will have mor air pockets and are more sensitive to water lodging. Go with the finer grade. (You can blend your Verm in a blender like I do:)

Follow the PF tek recipe and work until you find something that looks sort of like mine. In should have the same consistency as damp sand and break up much the same.

Here are 2 pictures of my jars:)





Spend some time in the kitchen mixing substrates until you get the hang of it. Dont stress. The kitchen work is a big part of the process:)

Hugs!

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